Genomic and phenotypic characterisation of antimicrobial resistance in carbapenem-resistant Acinetobacter baumannii hyperendemic clones CC1, CC15, CC79 and CC25

Accessory Genomic Epidemiology of Cocirculating Acinetobacter baumannii Clones

“…As with many other bacterial pathogens, WGS has been instrumental in properly understanding the transmission and phylogeography of many lineages of A. baumannii in several countries ( 13 – 21 ). WGS has also been employed to fully appreciate the processes generating genomic variation within the species ( 23 , 31 , 32 ).…”

“…WGS has been used to study the spread of clones of A. baumannii at a national level and even at the continental level ( 11 , 12 ). Over the last years, WGS has been used to analyze clone diversity within and between hospital settings in several parts of the world ( 13 – 21 ). For instance, there have been studies using WGS to study clones of this species within hospitals in some countries, such as Brazil, Lebanon, Malaysia, United Kingdom, and Vietnam ( 13 , 18 , 19 , 21 ).…”

“…When using WGS for epidemiological and phylogeographical investigations, the most common approach is to use core regions or core single nucleotide polymorphism (SNP) phylogenies and SNP differences between isolates ( 13 – 21 ). Phylogenies based on either core SNPs or core regions have been effective in most cases.…”

Caracterização fenotípica e molecular da resistência antimicrobiana e relação genética em isolados clínicos de clones de alto risco de Acinetobacter baumannii

“…For this study, a total of 79 carbapenem-resistant A. baumannii, one isolate per patient, from 26 different hospitals in Brazil, were selected based on different ApaI-pulsed field gel electrophoresis (PFGE) profiles and modified three-loci sequence typing (m3LST) . Antimicrobial resistance and clonality of such isolates were previously characterized (Camargo et al, 2020).…”

“…Os resultados são apresentados na Tabela 6. Com os picos gerados pelo MALDI-TOF, os dados foram analisados por PCA (Principal Components Analysis), o qual não agrupou as cepas com CC(Barcellos, 2021;Barcellos et al, 2020). Relação dos ST identificados por MLST (Esquema de Pasteur) e a distribuição da frequência dos isolados entre os ST encontrados entre os isolados de A. baumannii (n=63).…”