Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Kim, Shukho; Song, Hebok; Jin, Jong Sook; Lee, Weon Ju; Kim, Jungmin

doi:10.3390/antibiotics11081041

Cited by 12 publications

(11 citation statements)

References 50 publications

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“…2 , Table 3 , Supplemental Fig. S 2 , and Table S3 ) and agreed with other worldwide reports, with a rate of approximately 88% antibiotic sensitivity among clinical isolates 21 , 22 , 33 – 36 . Furthermore, although we noted a significant correlation in our C. acnes collection between the A1 SLST type and the antibiotic resistance strains, no correlation was detected between any SLST type and phage-resistance strains (Supplemental Fig.…”

Section: Discussionsupporting

confidence: 90%

“…One protein that did not follow this distribution was QPB11808.01 (as in PAVL33). This CDS was annotated as tape measure protein 22 , present in PAVL20, PAVL33, PAVL34, PAVL45, and on the other four phages (Fig. 1b and Supplemental Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…1). Interestingly, to the best of our knowledge, the numerous anti-C. acnes phages described to date belong to this family, with slight genomic variance 21,22,[33][34][35][36]42 . These observations raise unanswered questions regarding the existence of C. acnes phages from other families; why do they remain rare even if they do exist?…”

Section: Discussionmentioning

confidence: 99%

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Topical phage therapy in a mouse model of Cutibacterium acnes-induced acne-like lesions

et al. 2023

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Acne vulgaris is a common neutrophil-driven inflammatory skin disorder in which Cutibacterium acnes (C. acnes) is known to play a key role. For decades, antibiotics have been widely employed to treat acne vulgaris, inevitably resulting in increased bacterial antibiotic resistance. Phage therapy is a promising strategy to combat the growing challenge of antibiotic-resistant bacteria, utilizing viruses that specifically lyse bacteria. Herein, we explore the feasibility of phage therapy against C. acnes. Eight novel phages, isolated in our laboratory, and commonly used antibiotics eradicate 100% of clinically isolated C. acnes strains. Topical phage therapy in a C. acnes-induced acne-like lesions mouse model affords significantly superior clinical and histological scores. Moreover, the decrease in inflammatory response was reflected by the reduced expression of chemokine CXCL2, neutrophil infiltration, and other inflammatory cytokines when compared with the infected-untreated group. Overall, these findings indicate the potential of phage therapy for acne vulgaris as an additional tool to conventional antibiotics.

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Section: Discussionsupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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Topical phage therapy in a mouse model of Cutibacterium acnes-induced acne-like lesions

et al. 2023

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“…Almost all studied cell-free supernatant-medicinal oil combinations showed marked synergistic activity against S. aureus, E. coli, and Klebsiella pneumoniae. In another study, Kim et al (2022) reported the synergistic antibacterial potential of lactic acid bacteria combined with Curcuma longa rhizome extract against Cutibacterium acnes.…”

Section: Synergistic Effect Of Lab Antimicrobials With Other Compoundsmentioning

confidence: 99%

Recent development in the preservation effect of lactic acid bacteria and essential oils on chicken and seafood products

Sharma

Fidan²,

Özoğul³

et al. 2022

Front. Microbiol.

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Chicken and seafood are highly perishable owing to the higher moisture and unsaturated fatty acids content which make them more prone to oxidation and microbial growth. In order to preserve the nutritional quality and extend the shelf-life of such products, consumers now prefer chemical-free alternatives, such as lactic acid bacteria (LAB) and essential oils (EOs), which exert a bio-preservative effect as antimicrobial and antioxidant compounds. This review will provide in-depth information about the properties and main mechanisms of oxidation and microbial spoilage in chicken and seafood. Furthermore, the basic chemistry and mode of action of LAB and EOs will be discussed to shed light on their successful application in chicken and seafood products. Metabolites of LAB and EOs, either alone or in combination, inhibit or retard lipid oxidation and microbial growth by virtue of their principal constituents and bioactive compounds including phenolic compounds and organic acids (lactic acid, propionic acid, and acetic acid) and others. Therefore, the application of LAB and EOs is widely recognized to extend the shelf-life of chicken and seafood products naturally without altering their functional and physicochemical properties. However, the incorporation of any of these agents requires the optimization steps necessary to avoid undesirable sensory changes. In addition, toxicity risks associated with EOs also demand the regularization of an optimum dose for their inclusion in the products.

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“…C. acnes is mainly found in the areas of the skin rich in sebaceous glands; it accounts for about 90% of the total skin microbiota making it the most abundant bacterium 1 , 2 . Its involvement in several disorders like eye infections, prostate cancer, acne vulgaris 3 sarcoidosis 4 and rarely bacterial endocarditis 5 has caused its status shifted from commensal bacterium to an opportunistic pathogen 6 . Acne vulgaris is the most abundant skin disorder mainly effecting adolescents.…”

Section: Introductionmentioning

confidence: 99%

Expression of a recombinant endolysin from bacteriophage CAP 10-3 with lytic activity against Cutibacterium acnes

Kim,

Hasnain,

Moon

2023

Sci Rep

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The bacteriophage CAP 10-3 forming plaques against Cutibacterium acnes which causes skin acne was previously isolated from human skin acne lesion. Incomplete whole genome sequence (WGS) of the bacteriophage CAP 10-3 was obtained and it had 29,643 bp long nucleotide with 53.86% GC content. The sequence was similar to C. acnes phage PAP 1-1 with a nucleotide sequence identity of 89.63% and the bacteriophage belonged to Pahexavirus. Bioinformatic analysis of the WGS predicted 147 ORFs and functions of 40 CDSs were identified. The predicted endolysin gene of bacteriophage CAP 10-3 was 858 bp long which was deduced as 285 amino acids (~ 31 kDa). The protein had the highest similarity with amino acid sequence of the endolysin from Propionibacterium phage PHL071N05 with 97.20% identity. The CAP 10-3 endolysin gene was amplified by PCR with primer pairs based on the gene sequence, cloned into an expression vector pET-15b and transformed into Escherichia coli BL21(DE3) strain. The predicted protein band (~ 33 kDa) for the recombinant endolysin was detected in an SDS-PAGE gel and western blot assay. The concentrated supernatant of cell lysate from E. coli BL21(DE3) (pET-15b_CAP10-3 end) and a partially purified recombinant CAP 10-3 endolysin showed antibacterial activity against C. acnes KCTC 3314 in a dose-dependent manner. In conclusion, the recombinant CAP 10-3 endolysin was successfully produced in E. coli strain and it can be considered as a therapeutic agent candidate for treatment of human skin acne.

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Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Cited by 12 publications

References 50 publications

Topical phage therapy in a mouse model of Cutibacterium acnes-induced acne-like lesions

Topical phage therapy in a mouse model of Cutibacterium acnes-induced acne-like lesions

Recent development in the preservation effect of lactic acid bacteria and essential oils on chicken and seafood products

Expression of a recombinant endolysin from bacteriophage CAP 10-3 with lytic activity against Cutibacterium acnes

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