Genomic and phylogenetic analysis of two novel bovine papillomaviruses, BPV-9 and BPV-10

Abstract

Eight bovine papillomavirus (BPV) types, BPV-1-8, have been classified, based on genome nucleotide sequence similarities, in the genera Deltapapillomavirus (BPV-1 and -2), Epsilonpapillomavirus (BPV-5 and -8), Xipapillomavirus (BPV-3, -4 and -6) and an unassigned genus (BPV-7). We report here the complete genome sequence of two new BPV types isolated from separate epithelial squamous papilloma lesions on cattle teats. The genomes are 7303 and 7399 bp in length, respectively, and both have genetic organization and consensus motifs typical of papillomaviruses. A neighbour-joining phylogenetic tree revealed that both viruses cluster with BPV-3, -4 and -6. Nucleotide sequence identities of the BPV L1 major capsid protein of these two new BPVs with BPV-3, their closest relative, are 74.2 and 71.2 %, respectively. These results suggest that both viruses are new BPV types in the genus Xipapillomavirus, and they are designated BPV-9 and BPV-10.Bovine papillomavirus (BPV) is an aetiological agent associated with several forms of cutaneous and mucosal papillomas (Campo, 2002). Six different types of BPVs have been distinguished on the basis of DNA sequence relatedness. Each BPV is associated with type-specific lesions (Jarrett et al., 1984): BPV-1 and BPV-2 are classified in the genus Deltapapillomavirus and infect the epithelium and dermis, giving rise to fibropapillomas; BPV-3, BPV-4 and BPV-6 are classified in the genus Xipapillomavirus and are strictly epitheliotropic, inducing true epithelial papillomas, and BPV-5 is classified in the genus Epsilonpapillomavirus and infects the epithelium and dermis, inducing both fibropapillomas and true epithelial papillomas of the skin (Bloch et al., 1994;Campo 2002;de Villiers et al., 2004). Recently, 16 putative new BPV types were partially cloned and sequenced from healthy skin swabs (Antonsson & Hansson, 2002;Ogawa et al., 2004). Two of the BPVs were characterized further by cloning and sequencing their complete genomes. Phylogenetic analysis showed that both viruses were new BPV types: one was designated BPV-7 and classified as a member of a new papillomavirus (PV) genus, and the other was designated BPV-8 and classified as a member of the genus Epsilonpapillomavirus Tomita et al., 2007). However, the tumorigenic potential of these two new BPV types and the other 14 putative new BPV types has not been elucidated. In a previous report, we identified two putative new BPV types, designated BPV-Type I and BPV-Type II, which may have caused an outbreak of bovine teat papillomatosis in Japan (Maeda et al., 2007). BPV-Type I and II were identified by PCR amplification of a 590 bp DNA fragment within the BPV genome L1 major capsid protein gene (GenBank accession no. AB253592 for type I; no. AB253595 for type II). However, the remaining BPV-Type I and II genome sequences have not been reported. In this study, the complete genome sequences of BPV-Type I and II were determined. These sequencing and phylogenetic analysis results showed that BPV-Type I and II are new BPV types (designated...

Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle

“…The detection of BPV-10 using PCR in the teat warts of Indian cattle is a novel finding as earlier studies detected only BPV-1 and -2 in the cutaneous warts of cattle (Pangty et al 2010;Kumar et al 2013a), buffalo Kumar et al 2013a) and yak (Bam et al 2012). The association of BPV-10 with the teat warts of cattle was also observed in cattle papillomatosis studies of other countries (Hatama et al 2008). BPV-1 was detected in the three cases of teat warts with rice grain-like morphological appearance, while BPV-10 was detected in both rice grain-like and small, elevated sessile growths.…”

“…These BPV-induced lesions are usually benign in nature and regress spontaneously but can progress to cancer under the impact of environmental co-factors. To date, 13 different types of BPVs have been identified worldwide and grouped into three genera: Xi papillomaviruses , Delta papillomaviruses (BPV-1, -2 and -13) and Epsilon papillomaviruses whose genomes seem to share similarities with both Xi and Delta papillomaviruses (De Villiers et al 2004;Ogawa et al 2007;Tomita et al 2007;Hatama et al 2008Hatama et al , 2011Zhu et al 2012;Lunardi et al 2013).…”

Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd

“…However, recent studies employing PCR with generic primers FAP59/FAP64 in combination with cloning and sequencing, have described 15 putative new BPV types (Forslund et al 1999, Antonsson & Hansson 2002, Ogawa et al 2004. After characterization of their complete genome sequences, four of these Japanese isolates were recently recognized as new viral types (BPV-7, -8, -9, and -10) , Hatama et al 2008). In addition, four putative new BPV types have been identified in cutaneous lesions from cattle herds in Parana state, Brazil (Claus et al 2008).…”

Sarcoide equino associado ao papilomavírus bovino BR-UEL-4

“…A família Papillomaviridae é composta por 18 gêneros, dos quais quatro são compostos por diferentes espécies de BPV, destacando-se os Deltapapillomavirus (BPV-1 e 2), Xipapillomavirus (BPV-3, 4, 6, 9 e 10), Epsilonpapillomavirus (BPV-5 e 8), e um gênero ainda não classificado (BPV-7) (HATAMA et al, 2008). A identificação e classificação das espécies virais baseiam-se na homologia de regiões genômicas, especialmente do gene L1, que codifica a proteína mais importante do capsídeo viral (BORZACCHIELLO & ROPERTO, 2008).…”

“…Recentemente, os BPV BR-UEL-2 a 5 foram identificados no Brasil, associados com papilomas na pele de bovinos (CLAUS et al, 2008). No entanto, não há relato da ocorrência desses papilomavírus em associação a lesões cutâneas em outras espécies animais (CLAUS et al, 2008;HATAMA et al, 2008). O objetivo deste trabalho foi relatar um caso de sarcoide associado ao BPV BR-UEL-4 em um equino, no Estado do Rio de Janeiro.…”