Genomic characterization of ESBL/AmpC-producing and high-risk clonal lineages of Escherichia coli and Klebsiella pneumoniae in imported dogs with shelter and stray background

Johansson, Venla; Nykäsenoja, Suvi; Myllyniemi, Anna-Liisa; Rossow, Heidi; Heikinheimo, Annamari

doi:10.1016/j.jgar.2022.05.021

Cited by 9 publications

(5 citation statements)

References 48 publications

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“…Another relevant result was the lack of susceptibility to fluoroquinolones, namely to enrofloxacin, but also to ciprofloxacin. High occurrence of fluoroquinolones resistance among ESBL-positive isolates has already been reported in humans by Kantele et al [ 60 ] and in dogs Johansson et al [ 61 ], who suggested that this phenomena can be due to conjugative spread of bla genes by plasmid transfer.…”

Section: Discussionmentioning

confidence: 85%

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

Matos

Cunha

Baptista³

et al. 2023

Antibiotics

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Antimicrobial resistance is a public health threat with an increasing expression in low- and middle-income countries such as Cape Verde. In this country, there is an overpopulation of dogs, which may facilitate the spread of resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. To clarify the role of dogs as reservoirs for the dissemination of this bacterial group, 100 rectal swab samples were collected from confined (n = 50) and non-confined (n = 50) dogs in Santiago and Boa Vista Islands, Cape Verde. These were analyzed using conventional bacteriological techniques for the detection of ESBL-producing Enterobacteriaceae and characterization of their pathogenic and resistance profiles. Twenty-nine samples displayed ESBL-positive bacteria, from which 48 ESBL-producing isolates were obtained and mostly identified as Escherichia coli. Multiple antimicrobial resistance indexes ranged from 0.18 to 0.70 and half of the isolates were classified as multidrug-resistant. Isolates were capable of producing relevant virulence factors, including biofilm, showing virulence indexes between 0.29 and 0.71. As such, dogs in Cape Verde may act as reservoirs of resistant bacteria, including pathogenic and zoonotic species, representing a public health concern. Although further investigation is needed, this study proposes the periodical analysis of dogs’ fecal samples to monitor resistance dissemination in the country, in a One-Health perspective.

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Section: Discussionmentioning

confidence: 85%

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

Matos

Cunha

Baptista³

et al. 2023

Antibiotics

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“…Country-specific findings indicated that Nigeria had a PPE of 33.4% and 11.9% for E. coli and K. pneumoniae , respectively. Other countries, including Sudan [ 29 ], India [ 9 ], Algeria [ 30 ], Ghana [ 31 ], Turkey [ 32 ], Japan [ 33 ], Canada [ 34 ], Finland [ 35 ], Taiwan [ 36 ], South Africa [ 37 ], Tanzania [ 38 ], Tunisia [ 39 ], India [ 40 ], and the Netherland [ 41 ] were not included in the meta-analysis due to the low number of studies.…”

Section: Discussionmentioning

confidence: 99%

“One Health” perspective on prevalence of co-existing extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae: a comprehensive systematic review and meta-analysis

Ramatla,

Mafokwane,

Lekota

et al. 2023

Ann Clin Microbiol Antimicrob

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Background The Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) bacterial isolates that produce extended-spectrum β-lactamases (ESBLs) contribute to global life-threatening infections. This study conducted a systematic review and meta-analysis on the global prevalence of ESBLs in co-existing E. coli and K. pneumoniae isolated from humans, animals and the environment. Methods The systematic review protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO) [ID no: CRD42023394360]. This study was carried out following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. One hundred and twenty-six eligible studies published on co-existing antibiotic resistance in E. coli and K. pneumoniae between 1990 and 2022 were included. Results The pooled prevalence of ESBL-producing E. coli and K. pneumoniae was 33.0% and 32.7% for humans, 33.5% and 19.4% for animals, 56.9% and 24.2% for environment, 26.8% and 6.7% for animals/environment, respectively. Furthermore, the three types of resistance genes that encode ESBLs, namely blaSHVblaCTX−M,blaOXA, and blaTEM, were all detected in humans, animals and the environment. Conclusions The concept of “One-Health” surveillance is critical to tracking the source of antimicrobial resistance and preventing its spread. The emerging state and national surveillance systems should include bacteria containing ESBLs. A well-planned, -implemented, and -researched alternative treatment for antimicrobial drug resistance needs to be formulated.

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“…In 1998, the first ESBL strain was detected in bacteria of animal origin, an E. coli carrier of the SHV-12 gene [ 91 ]. Currently, there are several authors who report the presence of these bacteria in different animal species, emphasizing transmission to humans [ 26 , 28 , 29 , 87 , 92 , 93 , 94 , 95 , 96 , 97 ]. A study carried out from rectal swab samples in pairs of healthy mares and newborn foals reported the presence of ESBL in Escherichia coli strains in 25% and 29%, respectively, noting that during hospitalization this number increased significantly [ 98 ], the same as previously reported [ 99 ], suggesting an association with high use of antimicrobials, even in untreated animals [ 26 ], and nosocomial acquisition of ESBL in a hospital setting [ 100 , 101 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares

Thomson,

García,

Río

et al. 2023

Pathogens

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Antibiotic-resistant bacteria are a growing concern for human and animal health. The objective of this study was to determine the antimicrobial resistance and extended-spectrum beta-lactamase genes in Enterobacterales, Pseudomonas spp. and Acinetobacter spp. isolates from the uterus of healthy mares. For this purpose, 21 mares were swabbed for samples, which were later seeded on blood agar and MacConkey agar. The isolates were identified using MALDI-TOF and the antimicrobial susceptibility test was performed using the Kirby–Bauer technique. To characterize the resistance genes, a polymerase chain reaction (PCR) scheme was performed. Of the isolates identified as Gram-negative, 68.8% were Enterobacterales, represented by E. coli, Enterobacter cloacae, Citrobacter spp., and Klebsiella pneumoniae; 28.1% belonged to the genus Acinetobacter spp.; and 3.1% to Pseudomonas aeruginosa. A 9.3% of the isolates were multidrug-resistant (MDR), presenting resistance to antibiotics from three different classes, while 18.8% presented resistance to two or more classes of different antibiotics. The diversity of three genes that code for ESBL (blaTEM, blaCTX-M and blaSHV) was detected in 12.5% of the strains. The most frequent was blaSHV, while blaTEM and blaCTX-M were present in Citrobacter spp. and Klebsiella pneumoniae. These results are an alarm call for veterinarians and their environment and suggest taking measures to prevent the spread of these microorganisms.

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Genomic characterization of ESBL/AmpC-producing and high-risk clonal lineages of Escherichia coli and Klebsiella pneumoniae in imported dogs with shelter and stray background

Cited by 9 publications

References 48 publications

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern

“One Health” perspective on prevalence of co-existing extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae: a comprehensive systematic review and meta-analysis

Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares

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