1982
DOI: 10.1002/j.1460-2075.1982.tb01337.x
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Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L

Abstract: In an attempt to isolate the transposable genetic element Ds from Zea mays L., we cloned DNA fragments hybridizing to a cDNA clone derived from the sucrose synthase gene in a λ vector (λ::Zm Sh). The fragments cloned from wild‐type and from the Ds‐induced mutant sh‐m5933 (λ::Zm sh‐m5933) share a segment 6 kb long while a contiguous segment of 15 kb of λ::Zm sh‐m5933 (mutant‐derived DNA) does not hybridize to the DNA segment cloned from the wild‐type. Restriction maps are given, and the junction point between t… Show more

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Cited by 71 publications
(27 citation statements)
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“…Simple Ds produces an Our results strongly support the inference from work with maize that double Ds is involved in specific chromosome breakage (Courage-l ebbe et al, 1983;Doring et al, 1989). In maize, where there are many (30 to 50) copies of Ds sequences present in most genomes (Geiser et al, 1982;Fedoroff et al, 1983), it is difficult to prove that a particular Ds element confers a given phenotype. The allele sh-m5933 is associated with chromosome breakage .…”
Section: Discussionsupporting
confidence: 77%
See 1 more Smart Citation
“…Simple Ds produces an Our results strongly support the inference from work with maize that double Ds is involved in specific chromosome breakage (Courage-l ebbe et al, 1983;Doring et al, 1989). In maize, where there are many (30 to 50) copies of Ds sequences present in most genomes (Geiser et al, 1982;Fedoroff et al, 1983), it is difficult to prove that a particular Ds element confers a given phenotype. The allele sh-m5933 is associated with chromosome breakage .…”
Section: Discussionsupporting
confidence: 77%
“…This has proved difficult in maize because there are many copies of Ds sequences in most genomes (Geiser et al, 1982;Fedoroff et al, 1983). In maize, the structures that can be observed are limited to those that occur naturally, whereas in heterologous hosts, nove1 structures can be engineered and tested.…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, two different sets of PCR primers are required to amplify the upper and the lower strand of the reacted DNA, respectively (Table 1 and Figure 1). The maize genome contains hundreds of sequences with homology to Ac (Geiser et al, 1982;Theres et al, 1987). Many of these are Ds elements and have the same terminal sequences as Ac.…”
Section: Experimental Designmentioning
confidence: 99%
“…The sucrose synthase corresponding to Shl gene is a homotetramer composed of subunits of 92 kD which is mainly expressed in the endosperm (5,27,30). The genes have been cloned and sequenced (11,12,30 'Abbreviation: SS, sucrose synthase.…”
mentioning
confidence: 99%