Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences

Hagras, Adel Abdel Aziz; Kishii, Masahiro; Tanaka, Hiroyuki; Sato, Kazuhiro; Tsujimoto, Hisashi

doi:10.1266/ggs.80.147

Cited by 29 publications

(26 citation statements)

References 26 publications

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“…Hybrids between H. vulgare and H. chilense are possible, but are always sterile, and chromosome doubling to produce amphiploids has been unsuccessful (Thomas and Pickering 1985). Distinct diVerences at the chromosome level between H. chilense and H. vulgare were found by FISH using repetitive sequence probes (Hagras et al 2005b).…”

Section: Discussionmentioning

confidence: 99%

“…Codes, types and numbers of markers used are given in Table 1. Primer sequences and PCR conditions for all markers were taken from the references cited in Table 1 Hagras et al (2005b), were screened for polymorphism. Primer sequences and PCR conditions for these markers were according to these authors.…”

Section: Ssr Sts and Est-ssr Analysesmentioning

confidence: 99%

“…Barley expressed sequence tags (ESTs) were also assessed for polymorphism between wheat and ten alien Triticeae species, including H. chilense (Hagras et al 2005a). Polymorphic EST markers between common wheat and H. chilense were located to respective chromosomes of H. chilense using wheat-H. chilense addition lines (Hagras et al 2005b). However, the arm locations of these markers on the homoeologous chromosomes of H. chilense and H. vulgare are not known.…”

Section: Introductionmentioning

confidence: 99%

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A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST-SSR molecular markers

Said

Cabrera

2009

Euphytica

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The construction of a physical map of chromosome 4H ch from Hordeum chilense containing molecular markers capable of detecting segments of this chromosome in a wheat background would be very useful for marker-assisted introgression of 4H ch chromatin into both durum and common wheat. With this aim, the applicability of 106 barley chromosome 4H primers (62 SSRs and 44 STSs) to amplify markers showing polymorphism between H. chilense and both common or bread and durum wheat was investigated. Twenty-Wve SSR (40.3%) and six STS (13.6%) barley primer pairs consistently ampliWed H. chilense products. Eight SSR (12.9%) and four STS (9.1%) barley primers were polymorphic between H. chilense and both common and durum wheat, 10 of them (6 SSRs and 4 STSs) were located on chromosome 4H ch using both the addition line of chromosome 4H ch in Chinese Spring wheat and a tritordeum line (an amphiploid between H. chilense and T. turgidum) nullisomic for chromosome 4H ch . Additionally, 18 EST-SSR barley markers previously located on chromosome 4H ch were screened for polymorphism; 15 were polymorphic between H. chilense and both durum and common wheat. For physical mapping we used a ditelosomic tritordeum line for the short arm of chromosome 4H ch and a tritordeum line homozygous for a 70% terminal deletion of the long arm of 4H ch . A total of 25 markers (6 SSRs, 4 STSs and 15 ESTSSRs) were mapped to chromosome 4H ch . Eight markers were allocated on the 4H ch S, eight were mapped in the 30% proximal region of 4H ch L and nine were on the 70% distal region of 4H ch L, respectively. Arm location on barley chromosome 4H was also carried out using both 4HS and 4HL ditelosomic addition lines in wheat. All markers mapped may have a role in marker-assisted introgression of chromatin segments of chromosome 4H ch in both durum and common wheat backgrounds.

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Section: Discussionmentioning

confidence: 99%

Section: Ssr Sts and Est-ssr Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST-SSR molecular markers

Said

Cabrera

2009

Euphytica

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“…The digoxigenin-and biotin-labeled probes were detected with anti-digoxigeninFab fragment (green) and Cy3 Labeled Streptavidin (red), respectively. FISH and GISH was conducted as described by Hagras et al (2005). Table 1 shows the summary of the screening of rearranged B chromosomes by GISH/FISH analysis.…”

Section: Methodsmentioning

confidence: 99%

Dissection of rye B chromosomes, and nondisjunction properties of the dissected segments in a common wheat background

et al. 2008

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The rye B chromosome is a supernumerary chromosome that increases in number in its host by directed postmeiotic drive. Two types of rye B chromosomes that had been introduced into common wheat were dissected into separate segments by the gametocidal system to produce a number of rearranged B chromosomes, such as telosomes, terminal deletions and translocations with wheat chromosomes. A total of 13 dissected B chromosomes were isolated in common wheat, and were investigated for their nondisjunction properties. Rearranged B chromosomes, separated from their B-specific repetitive sequences on the distal part of the long arm, did not undergo nondisjunction, and neither did a translocated wheat chromosome carrying a long-arm distal segment containing the Bspecific repetitive sequences. However, such rearranged B chromosomes, missing their B-specific sequences could undergo nondisjunction when they coexisted with the standard B chromosome or a wheat chromosome carrying the B-specific sequences. Deficiencies of the short arm did not completely abolish the nondisjunction properties of the B chromosome, but did reduce the frequency of nondisjunction. These results confirmed previous suggestions that the directed nondisjunction of the rye B chromosome is controlled by two elements, pericentromeric sticking sites and a trans-acting element carried at the distal region of the long arm of the B chromosome. Additionally, it is now shown that the distal region of the long arm of the B chromosome which provides this function is that which carries the B-specific repetitive sequences.

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“…del1H ch -2) terminal deletion lines. In addition, we tested 29 EST-SSR primer pairs (coded BAWU) that had previously been assigned to chromosome 1H ch (Hagras et al 2005), but not to their respective chromosome arms.…”

Section: Est-ssr Molecular Analysismentioning

confidence: 99%

Sub-arm location of prolamin and EST-SSR loci on chromosome 1Hch from Hordeum chilense

et al. 2010

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Hordeum chilense Roem. et Schult. is a diploid wild South American barley that contains genes of interest for cereal breeding, many of them located on chromosome 1H ch . In the current study, two H. chilense-wheat addition lines with deletions in the 1H ch chromosome were used for sub-arm localization of five prolamin (glutenin and gliadin) loci and 33 EST-SSR marker loci on chromosome 1H ch . The two sets of markers were distributed across five sub-arm chromosome regions. Three glutenin loci (Glu-H ch 2, Glu-H ch 3, Glu-H ch 4) together with the gliadin locus Gli-H ch 1 were located on the distal 20% of the 1H ch S arm, whereas the glutenin locus Glu-H ch 1 was on the proximal 88% region of 1H ch L. Among 33 EST-SSR marker loci, 7 (21.2%) were on the 1H ch S arm and, of them, 3 (9.1%) were on the distal 20% end and 4 (12.1%) on the proximal 80% region. The 26 loci (78.8%) on 1H ch L were distributed across three different regions: 18 (78.8%) in the proximal 88%, 3 (9.1%) in the distal 12% and 5 (15.2%) in a region less than 12% from the distal end. The deletions in the 1H ch chromosome added to the common wheat background were thus shown to be useful for determining the sub-arm location of EST-SSR and prolamin loci. This could facilitate the identification of molecular markers linked to genes of agronomic interest and the isolation of such genes for use in common wheat improvement.

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Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences

Cited by 29 publications

References 26 publications

A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST-SSR molecular markers

A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST-SSR molecular markers

Dissection of rye B chromosomes, and nondisjunction properties of the dissected segments in a common wheat background

Sub-arm location of prolamin and EST-SSR loci on chromosome 1Hch from Hordeum chilense

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