2021
DOI: 10.1016/j.bios.2020.112864
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Genomic DNA i-motifs as fast sensors responsive to near-physiological pH microchanges

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Cited by 7 publications
(16 citation statements)
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“…Consistently with previous reports on exogenous iMs, including genomic sequences and genome-derived nonratiometric sensors, FAM-C5T-TAMRA and FAM-C5T propyl -TAMRA tended to localize in the nucleus (Figure a). Removal of a single label or replacement of the iM with a pH-insensitive T20 spacer diminished the fluorescence signal in the nucleoplasm (Figures a and S16).…”
Section: Resultsmentioning
confidence: 99%
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“…Consistently with previous reports on exogenous iMs, including genomic sequences and genome-derived nonratiometric sensors, FAM-C5T-TAMRA and FAM-C5T propyl -TAMRA tended to localize in the nucleus (Figure a). Removal of a single label or replacement of the iM with a pH-insensitive T20 spacer diminished the fluorescence signal in the nucleoplasm (Figures a and S16).…”
Section: Resultsmentioning
confidence: 99%
“…The following set of oligodeoxyribonucleotides (ODNs) was used in this study: iM-forming dual-labeled ODNs FAM-C 5 XC 5 XC 5 XC 5 -TAMRA, where X is a native T residue, a 1′,2′-dideoxyribose residue, or an alkyl linker (ratiometric sensors); control iM-forming ODN FAM-C 23 -BHQ1, which has been previously named ATX after the C 23 -containing gene ATXN2L and calibrated for pH sensing in A549 cells (nonratiometric sensor); control iM-forming single-labeled ODNs FAM-C 5 TC 5 TC 5 TC 5 and C 5 TC 5 TC 5 TC 5 -TAMRA; control non-iM dual-labeled ODNs FAM-T 20 -TAMRA and FAM-CT 20 C-TAMRA. …”
Section: Methodsmentioning
confidence: 99%
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“…In particular, the i-motif, which is a quadruplex structure formed by the chimerism of cytosine-cytosine base pairs in an antiparallel manner, has been explored. 22 Since the i-motif structure is stabilized by the formation of hydrogen bonds between protonated cytosine and non-protonated cytosine, its spatial structure is greatly affected by the pH value. 23 The i-motif structure is mostly formed and stabilized under acidic conditions but not under neutral or alkaline conditions.…”
Section: Introductionmentioning
confidence: 99%
“…In the promoters of a number of genes, including genes of the family SHANK, not so long ago, C-rich sequences were discovered that form stable C-quadruplex structures-intercalated motifs (iM) [1][2][3]. Since the formation of the iM nucleus, which is parallel duplexes with hemiprotonated cytosine pairs, requires protonation of the N3 atom of cytosine residues, most structures of this type exist exclusively in a weakly acidic medium [4].…”
Section: Introductionmentioning
confidence: 99%