Genomic DNA microextraction: A method to screen numerous samples

Ramirez-Solis, Ramiro; Rivera-Pérez, Jaime A.; Wallace, James D.; Wims, Marie; Zheng, Hui; Bradley, Allan

doi:10.1016/0003-2697(92)90347-a

Cited by 209 publications

(114 citation statements)

References 17 publications

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“…Solid horizontal boxes represent the 5Ј and 3Ј arms. A phosphoglycerate kinase-hypoxanthine phosphoribosyltransferase expression cassette was used as the positive selectable marker, and an MC1tk expression cassette was used as a negative selectable marker as shown (31). B, BamHI; Bg, BglII; E, EcoRI; H, HindIII; P, PstI; Sp, SphI.…”

Section: Resultsmentioning

confidence: 99%

“…The linearized targeting vector (25 g) was electroporated into the HPRT-negative AB2.1 line of embryonic stem (ES) cells (10 7 ) and selected in hypoxanthine͞aminopterin͞thymidine (HAT) and 1-(2-deoxy-2-f luoro-␤-arabinofuranosyl)-5-iodouracil (FIAU) as described (30). HAT R , FIAU R ES cell clones were screened by Southern blot analysis (31) for correct targeting at the MBD locus. Germline transmission from chimeric males generated from three independent clones of ES cell lines was determined by Southern blot analysis as described (30).…”

Section: Methodsmentioning

confidence: 99%

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Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

Habib

Shi

Cuevas

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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We have developed mice deficient in membrane-bound dipeptidase (MBD, EC 3.4.13.19), the enzyme believed to be responsible for the conversion of leukotriene D 4 (LTD 4 ) to leukotriene E 4 (LTE 4 ). The MBD mutation generated by us was demonstrated to be a null mutation by Northern blot analysis and the absence of ␤-lactamase activity in lung, kidney, small intestine, and heart. MBD gene deletion had no effect on viability or fertility. The mutant mice retain partial ability to convert LTD 4 to LTE 4 , ranging from 80-90% of the wild-type values in small intestine and liver to 16% in kidney and 40% in lung, heart, and pancreas. MBD is also believed to function consecutively after ␥-glutamyl transpeptidase to cleave cystinyl-bis-glycine (cys-bis-gly) generated from glutathione cleavage. Our data indicate that kidney homogenates from MBD-deficient mice retain ϳ40% of their ability to cleave cys-bis-gly, consistent with only modest elevations (3-5-fold) of cys-bis-gly in urine from MBDdeficient mice. These observations demonstrate that the conversion of LTD 4 to LTE 4 and the degradation of cys-bis-gly are catalyzed by at least two alternative pathways (one of which is MBD) that complement each other to varying extents in different tissues.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

Habib

Shi

Cuevas

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…Genomic DNA from individual ES clones was directly isolated onto replica 96-well plates according to the procedure of Ramirez-Solis et al (23), dissolved in 30/A of restriction enzyme buffer (recommended by the supplier of enzyme) containing 1 mM spermidine and 100/~g/ml BSA, and digested with 20 U of either BamHI or XbaI (incubation 16 h, 37~ Genomic DNA was isolated from mouse tails by a standard protocol (24) using proteinase K digestion, phenol extraction, and ethanol precipitation. Restricted DNA from ES cell clones or mouse tails was resolved by electrophoresis in 1% agarose gels containing TAE buffer (40 mM Tris-acetate, 20 mM sodium acetate, 20 mM EDTA, pH 7.6), and transferred onto nylon membranes (Hybond-N; Amersham International, Little Chalfont, UK) by capillary blotting in 20x SSC (lx SSC is 0.15 M NaC1, 0.015 M sodium citrate).…”

Section: Genotyping Of Es Cell Lines and Mutantmentioning

confidence: 99%

The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nramp1 gene.

Vidal

Tremblay

Govoni

et al. 1995

The Journal of Experimental Medicine

555

337

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SummaryIn mice, natural resistance or susceptibility to infection with intracellular parasites is determined by a locus or group of loci on chromosome 1, designated Bcg, Lsh, and Ity, which controls early microbial replication in reticuloendothelial organs. We have identified by positional cloning a candidate gene for Beg, Nrampl, which codes for a novel macrophage-specific membrane transport protein. We have created a mouse mutant bearing a null allele at Nrampl, and we have analyzed the effect of such a mutation on natural resistance to infection. Targeted disruption of Nrampl has pleiotropic effects on natural resistance to infection with intracellular parasites, as it eliminated resistance to Mycobacterium boris, Leishmania donovani, and lethal Salmonella typhimurium infection, establishing that Nrampl, Bcg, Lsh, and It), are the same locus. Comparing the profiles of parasite replication in control and Nrampl -/-mice indicated that the NramplAse 169 allele of Beg s inbred strains is a null allele, pointing to a critical role of this residue in the mechanism of action of the protein. Despite their inability to control parasite growth in the early nonimmune phase of the infection, Nrampl -/-mutants can overcome the infection in the late immune phase, suggesting that Nrampl plays a key role only in the early part of the macrophage-parasite interaction and may function by a cytocidal or cytostatic mechanism distinct from those expressed by activated macrophages.

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“…Individual colonies were picked under 10-40 × magnification, grown on feeders in 96-well plates, and replica-plated, using one plate for DNA and the other as a frozen stock, as described (Ramirez-Solis et al 1992). In the case of mafK targeting, 68 colonies were screened by Southern blotting using a 5Ј external probe (a 0.6-kb XbaI fragment 2.0-kb 3Ј to exon IM), which identified 5 colonies with a 10.0-kb mutant BglII fragment in addition to the 6.9-kb endogenous fragment.…”

Section: Gene Targeting In Es Cellsmentioning

confidence: 99%

Impaired megakaryopoiesis and behavioral defects inmafG-null mutant mice

Shavit¹,

Motohashi²,

Onodera³

et al. 1998

Genes Dev.

103

127

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The small Maf proteins (MafG, MafK, and MafF), which serve as heterodimeric partner molecules of CNC family proteins for binding in vitro to MARE sites, have been implicated in the regulation of both transcription and chromatin structure, but there is no current evidence that the proteins fulfill these functions in vivo. To elucidate possible contributions of the small Maf proteins to gene regulation, we have ablated the mafG and mafK genes in mice by replacing their entire coding sequences with the Escherichia coli lacZ gene. mafG homozygous mutant animals exhibit impaired platelet formation accompanied by megakaryocyte proliferation, as well as behavioral abnormalities, whereas mafK-null mutant mice are phenotypically normal. Characterization of the mafG and mafK embryonic expression patterns show that their developmental programs are distinct and intersecting, but not entirely overlapping. These results provide direct evidence that the small Maf transcription factors are vital participants in embryonic development and cellular differentiation.

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Genomic DNA microextraction: A method to screen numerous samples

Cited by 209 publications

References 17 publications

Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nramp1 gene.

Impaired megakaryopoiesis and behavioral defects inmafG-null mutant mice

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Genomic DNA microextraction: A method to screen numerous samples

Cited by 209 publications

References 17 publications

Leukotriene D 4 and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

Leukotriene D 4 and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nramp1 gene.

Impaired megakaryopoiesis and behavioral defects inmafG-null mutant mice

Contact Info

Product

Resources

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Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice

Leukotriene D ₄ and cystinyl-bis-glycine metabolism in membrane-bound dipeptidase-deficient mice