Genomic Exploration of the Hemiascomycetous Yeasts: 11. <i>Kluyveromyces lactis</i>

Bolotin‐Fukuhara, Monique; Toffano‐Nioche, Claire; Artiguenave, François; Duchateau‐Nguyen, Guillemette; Lemaire, Marc; Marmeisse, Roland; Montrocher, R.; Robert, Caroline; Termier, Michel; Wincker, Patrick; Wésolowski-Louvel, Micheline

doi:10.1016/s0014-5793(00)02282-1

Cited by 65 publications

(35 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among 682 couples of genes [29], we observed 56.5% conserved synteny between the two yeasts. This value is close to S. kluyveri (53.5%) [30], K. lactis (47.3%) [31], and K. marxianus var. marxianus (49.8%) [32].…”

Section: 7supporting

confidence: 56%

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

et al. 2000

Self Cite

View full text Add to dashboard Cite

A genomic exploration of Kluyveromyces thermotolerans was performed by random sequence tag (RST) analysis. We sequenced 2653 RSTs corresponding to inserts sequenced from both ends. We performed a systematic comparison with a complete set of proteins from Saccharomyces cerevisiae, other completely sequenced genomes and SwissProt. We identified six mitochondrial genes and 1358^1496 nuclear genes by comparison with S. cerevisiae. In addition, 25 genes were identified by comparison with other organisms. This corresponds to about 24% of the estimated gene content of this organism. A lower level of conservation is observed with orthologues to genes of S. cerevisiae previously classified as orphans. Gene order was found to be conserved between S. cerevisiae and K. thermotolerans in 56.5% of studied cases. ß

show abstract

Section: 7supporting

confidence: 56%

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

et al. 2000

Self Cite

View full text Add to dashboard Cite

show abstract

“…This task will be facilitated by the recent availability of a large dataset of sequences corresponding to 47-56% of the K. lactis genome (Bolotin-Fukuhara et al, 2000) and to 16% of the K. marxianus genome (Llorente et al, 2000), which can be used to design new sequencing strategies for the remaining species of the group.…”

Section: Discussionmentioning

confidence: 99%

An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon

Belloch¹,

Fernández-Espinar²,

Querol³

et al. 2002

Yeast

View full text Add to dashboard Cite

In the present work, we analyse the sequences of the 5.8S rRNA gene and the two internal transcribed spacers 1 and 2 (5.8S-ITS region), obtained from 39 strains belonging to the species Kluyveromyces aestuarii, K. dobzhanskii, K. lactis and K. marxianus, K. nonfermentans and K. wickerhamii, to solve the phylogenetic relationships among these species and also to determine the possible genetic basis for the delimitation of the two currently accepted K. lactis varieties: lactis, including lactose-positive strains isolated from dairy products, and drosophilarum, comprising lactose-negative strains isolated from insects and plant exudates. The determination of the phylogenetic relationships within the species K. lactis, together with the examination of the electrophoretic karyotypes and phenotypic characterization of strains representatives of K. lactis var. lactis and var. drosophilarum, allowed differentiation of two groups of strains. The first, and ancestral, group comprises lactose-negative strains isolated from natural habitats in North America. The second, and derived, group includes both lactose-negative strains isolated from natural habitats in Europe and wine fermentation in South Africa, and lactose-positive strains associated with dairy products. These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations.

show abstract

“…We also found the homologue of dihydroorotate dehydrogenase (PYRD) from Arabidopsis thaliana, another gene involved in pyrimidine catabolism, probably in the ¢rst step of pyrimidine degradation (Piskur, personal communication). Homologues of these genes were also identi¢ed in K. thermotolerans [28], Y. lipolytica [30], K. lactis [31] and P. angusta [32], but not in the other Saccharomyces species. The presence/absence of this pathway is consistent with the phylogenetic separation of the species.…”

Section: Comparison With Other Genomesmentioning

confidence: 96%

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

et al. 2000

Self Cite

View full text Add to dashboard Cite

The genome of Saccharomyces kluyveri was explored through 2528 random sequence tags with an average length of 981 bp. The complete nuclear ribosomal DNA unit was found to be 8656 bp in length. Sequences homologous to retroelements of the gypsy and copia types were identified as well as numerous solo long terminal repeats. We identified at least 1406 genes homologous to Saccharomyces cerevisiae open reading frames, with on average 58.1% and 72.4% amino acid identity and similarity, respectively. In addition, by comparison with completely sequenced genomes and the SwissProt database, we found 27 novel S. kluyveri genes. Most of these genes belong to pathways or have functions absent from S. cerevisiae, such as the catabolic pathway of purines or pyrimidines, melibiose fermentation, sorbitol utilization, or degradation of pollutants. The sequences are deposited in EMBL under the accession numbers AL404849^AL407376. ß

show abstract

Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis

Cited by 65 publications

References 31 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

Contact Info

Product

Resources

About