Genomic Exploration of the Hemiascomycetous Yeasts: 17. <i>Yarrowia lipolytica</i>

Casarégola, Serge; Neuvéglise, Cécile; Lépingle, Andrée; Bon, Elisabeth; Feynerol, C; Artiguenave, François; Wincker, Patrick; Gaillardin, Claude

doi:10.1016/s0014-5793(00)02288-2

Cited by 100 publications

(68 citation statements)

References 32 publications

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“…Our results offer one of the best examples of the conservation of synteny and gene orientation between different and yeast species. The lack of homology of three Y. lipolytica genes (out of eight) with the gene sequences described in the databases are in good agreement with the only 1187 genes identified after (Casaregola et al 2000). In all cases the highest identities were obtained between the two most closely related yeasts (S. cerevisiae and K. lactis), the data being consistent with their phylogenetic position (Figure 4; Barns et al, 1991, Bolotin-Fukuhara et al, 2000, Llorente et al, 2000.…”

Section: Orfs and Orf Productssupporting

confidence: 79%

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

Sánchez

Domínguez

2001

Yeast

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We have determined the sequence of a 10275 bp DNA segment of Yarrowia lipolytica located on chromosome VI. The sequence contains six complete open reading frames (ORFs) longer than 100 amino acids and two more partial ORFs at both ends. Two of the ORFs encode for the well-characterized genes YlURA5 (orotate phosphoribosyltransferase) and YlSEC65 (encoding a subunit of the signal recognition particle). These two genes show an identical organization-located on opposite strands and in opposite orientations-in four yeast species: Saccharomyces cerevisiae, Kluyveromyces lactis, Candida albicans and Y. lipolytica. One ORF and the two partial ORFs code for putative proteins showing significant homology with proteins from other organisms. YlVI-108w (partial) and YlVI-103w show 39% and 54% identity, respectively, with YDR430c and YHR088w from S. cerevisiae. YlVI-102c (partial) shows significant homology with a matrix protein, lustrin A from Haliotis rufescens, and with the PGRS subfamily (Gly-rich proteins) of Mycobacterium tuberculosis. The three remaining ORFs show weak or nonsignificant homology with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AI006754.

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Section: Orfs and Orf Productssupporting

confidence: 79%

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

Sánchez

Domínguez

2001

Yeast

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“…One great interest in this species is that it has become a model for many steps of non-polar substrate metabolism, such as the action of lipases, cytochrome P450, acyl-CoA oxidases and the biology of peroxisomes. The data existing at all these levels and the genome knowledge to come (Casaregola et al 2000) will help to develop new applications for this unconventional yeast.…”

Section: Resultsmentioning

confidence: 99%

Catabolism of hydroxyacids and biotechnological production of lactones by Yarrowia lipolytica

Waché¹,

Aguedo²,

Nicaud

et al. 2003

Appl Microbiol Biotechnol

116

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The g-and d-lactones of less than 12 carbons constitute a group of compounds of great interest to the flavour industry. It is possible to produce some of these lactones through biotechnology. For instance, g-decalactone can be obtained by biotransformation of methyl ricinoleate. Among the organisms used for this bioproduction, Yarrowia lipolytica is a yeast of choice. It is well adapted to growth on hydrophobic substrates, thanks to its efficient and numerous lipases, cytochrome P450, acylCoA oxidases and its ability to produce biosurfactants. Furthermore, genetic tools have been developed for its study. This review deals with the production of lactones by Y. lipolytica with special emphasis on the biotransformation of methyl ricinoleate to g-decalactone. When appropriate, information from the lipid metabolism of other yeast species is presented.

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“…We also found the homologue of dihydroorotate dehydrogenase (PYRD) from Arabidopsis thaliana, another gene involved in pyrimidine catabolism, probably in the ¢rst step of pyrimidine degradation (Piskur, personal communication). Homologues of these genes were also identi¢ed in K. thermotolerans [28], Y. lipolytica [30], K. lactis [31] and P. angusta [32], but not in the other Saccharomyces species. The presence/absence of this pathway is consistent with the phylogenetic separation of the species.…”

Section: Comparison With Other Genomesmentioning

confidence: 96%

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

et al. 2000

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The genome of Saccharomyces kluyveri was explored through 2528 random sequence tags with an average length of 981 bp. The complete nuclear ribosomal DNA unit was found to be 8656 bp in length. Sequences homologous to retroelements of the gypsy and copia types were identified as well as numerous solo long terminal repeats. We identified at least 1406 genes homologous to Saccharomyces cerevisiae open reading frames, with on average 58.1% and 72.4% amino acid identity and similarity, respectively. In addition, by comparison with completely sequenced genomes and the SwissProt database, we found 27 novel S. kluyveri genes. Most of these genes belong to pathways or have functions absent from S. cerevisiae, such as the catabolic pathway of purines or pyrimidines, melibiose fermentation, sorbitol utilization, or degradation of pollutants. The sequences are deposited in EMBL under the accession numbers AL404849^AL407376. ß

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Genomic Exploration of the Hemiascomycetous Yeasts: 17. Yarrowia lipolytica

Cited by 100 publications

References 32 publications

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

Gene order in a 10 275 bp fragment of Yarrowia lipolytica, including adjacent YlURA5 and YlSEC65 genes conserved in four yeast species

Catabolism of hydroxyacids and biotechnological production of lactones by Yarrowia lipolytica

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

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