Genomic fingerprinting of 80 strains from the WHO multicenter international typing study of Listeria monocytogenes via pulsed-field gel electrophoresis (PFGE)

Brosch, Roland; Brett, Maggie; Catimel, B.; Luchansky, John B.; Ojeniyi, B.; Rocourt, J.

doi:10.1016/s0168-1605(96)01147-6

Cited by 104 publications

(50 citation statements)

References 9 publications

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“…Similarly, the same enzymes could not differentiate isolates from the outbreak in Los Angeles in 1985 and from the outbreak in Vaud, Switzerland, from 1983 to 1987 (5). Our results are thus consistent with previous results, which show the highly clonal nature of the serotype 4b clonal groups (5,29). Sensitive subtyping of these isolates represents a significant challenge and may require novel, possibly genomicsbased, approaches.…”

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confidence: 92%

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

et al. 2001

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To develop improved automated subtyping approaches for Listeria monocytogenes, we characterized the discriminatory power of different restriction enzymes for ribotyping. When 15 different restriction enzymes were used for automated ribotyping of 16 selected L. monocytogenes isolates, the restriction enzymes EcoRI, PvuII, and XhoI showed high discriminatory ability (Simpson's index of discrimination > 0.900) and produced complete and reproducible restriction cut patterns. These three enzymes were thus evaluated for their ability to differentiate among isolates representing the two major serotype 4b epidemic clones, those having ribotype reference pattern DUP-1038 (51 isolates) and those having pattern DUP-1042 (20 isolates). Among these isolates, PvuII provided the highest discrimination for a single enzyme (nine different subtypes; index of discrimination ‫؍‬ 0.518). A combination of PvuII and XhoI showed the highest discriminatory ability (index of discrimination ‫؍‬ 0.590) for these isolates. A group of 44 DUP-1038 isolates and a group of 12 DUP-1042 isolates were identical to each other even when the combined data for all three enzymes were used. We conclude that automated ribotyping using different enzymes allows improved discrimination of L. monocytogenes isolates, including epidemic serotype 4b strains. We furthermore confirm that most of the isolates representing the genotypes linked to the two major epidemic L. monocytogenes clonal groups form two genetically homogeneous groups.

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confidence: 92%

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

et al. 2001

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“…Early multilocus enzyme electrophoresis (MEE)-based subtyping (3) and restriction enzyme analysis (57) showed that strains from many different outbreaks were closely related even though those outbreaks were geographically and temporally distinct. These findings were also supported by data generated using ribotyping (12), virulence gene polymorphism analysis (59), and pulsed-field gel electrophoresis (PFGE) (4,5,28,31). Kathariou (30,31) subsequently compared those studies and defined four epidemic clones of L. monocytogenes (epidemic clone I [ECI], ECIa, ECII, and ECIII).…”

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confidence: 83%

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

2007

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“…Genomic macrorestriction based on rare-cutting endonucleases followed by pulsed-field gel electrophoresis (PFGE) remains as the gold standard test for routine molecular subtyping of most clinically important foodborne pathogens, including L. monocytogenes (Brosch et al, 1996;Graves & Swaminathan, 2001;Gerner-Smidt et al, 2006;Fugett et al, 2007;Ortiz et al, 2010). Analysis of data using bioinformatics software clusters isolates according to banding pattern similarity in a genetic distance tree known as a dendrogram (Nightingale, 2010) (Fig.…”

Section: Ecology Of Listeria Monocytogenes and Molecular Subtyping Mementioning

confidence: 99%

Review. Dairy farm management and production practices associated with the presence of Listeria monocytogenes in raw milk and beef

Santorum

Garcia

López-Alonso

et al. 2012

Span. j. agric. res.

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Human listeriosis is a severe foodborne disease caused by Listeria monocytogenes. It is a zoonosis that represents a significant concern for the food industry due to the high mortality rate it causes and the fact that the organism is capable of growing at refrigeration temperatures. Dairy products and ready-to-eat meats are among the foods most often involved in listeriosis outbreaks. Listeria is a common contaminant in the dairy environment, both on the farm and in the processing plant. The main sources of L. monocytogenes in dairy farms are manure and improperly fermented silage. If silage crops are grown on contaminated land, a new cycle of silage contamination and faecal shedding by ruminants that consume such silage may ensue. High loads of L. monocytogenes produced in farm environments may thus represent a primary source for the introduction of this pathogen into the human food supply chain; dairy cows would represent a reservoir for the bacterium, and raw milk and beef would represent the main vehicles for its transmission from dairy farms to humans. Even if contamination originates in post-processing environments, contaminated raw foods may still represent a vehicle for introducing L. monocytogenes into food processing plants. Molecular typing methods have confirmed that common strains of L. monocytogenes are present in dairy farm-associated isolates and isolates from both human epidemic and sporadic cases. Pre-harvest (on-farm) control of listeriosis should be based mainly on the control of manure, silage, herd health and milking practices.Additional key words: animal reservoir; dairy primary production; environmental sources; food vehicles; listeriosis. ResumenRevisión. Tipos de gestión y producción de las granjas de ganado vacuno lechero relacionados con la presencia de Listeria monocytogenes en la leche y la carne La listeriosis humana es una grave enfermedad transmitida por alimentos y causada por Listeria monocytogenes. Se trata de una zoonosis que supone una gran preocupación para la industria alimentaria debido a su alta tasa de mortalidad y al hecho de que el microorganismo es capaz de crecer a temperaturas de refrigeración. Listeria es un contaminante habitual en las granjas y plantas de productos lácteos. En las granjas las fuentes principales de L. monocytogenes son el estiércol y los ensilados mal fermentados. Si la cosecha utilizada para producir ensilados procede de campos contaminados, puede comenzar un nuevo ciclo de contaminación de los ensilados y liberación del patógeno en las heces de los animales. El ganado vacuno lechero representa uno de los principales reservorios de este microorganismo y la leche y la carne representarían los principales vehículos para su transmisión desde la granja de producción de leche al ser humano. Incluso cuando la contaminación del alimento procede de etapas posteriores al procesado, los alimentos crudos contaminados también representan uno de los vehículos de entrada de L. monocytogenes en las plan-

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Genomic fingerprinting of 80 strains from the WHO multicenter international typing study of Listeria monocytogenes via pulsed-field gel electrophoresis (PFGE)

Cited by 104 publications

References 9 publications

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains ofListeria monocytogenes

Review. Dairy farm management and production practices associated with the presence of Listeria monocytogenes in raw milk and beef

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