Genomicin situhybridization analysis betweenRubus coreanusand its relatives inRubus(Sect.Idaeobatus)

Wang, Y.; Nan, Hong; Chen, Q.; He, Wei; Zhang, L.; Wang, X.-R.; Tang, Haoru

doi:10.1080/11263504.2014.941030

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…The GISH technique has been successfully used to illustrate the relationships between related species in woody plants with small chromosomes [20,21]. The tetraploid Chinese cherry genome size was estimated to be 294 Mb, with repetitive sequences accounting for about 31.95% of the genome [48].…”

Section: Gish Analysis Between Chinese Cherry and Cerasus Diploid Rel...mentioning

confidence: 99%

“…The fluorescence in situ hybridization (FISH) technique, employing repetitive DNA probes, has been widely used to identify auto-or allopolyploids in plants [17][18][19]. Genomic in situ hybridization (GISH), a variant of FISH, utilizes whole genomic probes for suppressive hybridizations to visualize individual genomes in hybrids and polyploids, illustrating the genetic relationship between relative species [20,21]. The hybridization signals not only represent common DNA sequences, but also demonstrate genomic homology between the two species [22].…”

Section: Introductionmentioning

confidence: 99%

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Autotetraploid Origin of Chinese Cherry Revealed by Chromosomal Karyotype and In Situ Hybridization of Seedling Progenies

Wang,

Li,

Feng

et al. 2023

Plants

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Polyploidy is considered a driving force in plant evolution and diversification. Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don], an economically important fruit crop native to China, has evolved at the tetraploid level, with a few pentaploid and hexaploid populations. However, its auto- or allo-polyploid origin remains unclear. To address this issue, we analyzed the ploidy levels and rDNA chromosomal distribution in self- and open-pollinated seedling progenies of tetraploid and hexaploid Chinese cherry. Genomic in situ hybridization (GISH) analysis was conducted to reveal the genomic relationships between Chinese cherry and diploid relatives from the genus Cerasus. Both self- and open-pollinated progenies of tetraploid Chinese cherry exhibited tetraploids, pentaploids, and hexaploids, with tetraploids being the most predominant. In the seedling progenies of hexaploid Chinese cherry, the majority of hexaploids and a few pentaploids were observed. A small number of aneuploids were also observed in the seedling progenies. Chromosome 1, characterized by distinct length characteristics, could be considered the representative chromosome of Chinese cherry. The basic Chinese cherry genome carried two 5S rDNA signals with similar intensity, and polyploids had the expected multiples of this copy number. The 5S rDNA sites were located at the per-centromeric regions of the short arm on chromosomes 4 and 5. Three 45S rDNA sites were detected on chr. 3, 4 and 7 in the haploid complement of Chinese cherry. Tetraploids exhibited 12 signals, while pentaploids and hexaploids showed fewer numbers than expected multiples. Based on the GISH signals, Chinese cherry demonstrated relatively close relationships with C. campanulata and C. conradinae, while being distantly related to another fruiting cherry, C. avium. In combination with the above results, our findings suggested that Chinese cherry likely originated from autotetraploidy.

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Section: Gish Analysis Between Chinese Cherry and Cerasus Diploid Rel...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Autotetraploid Origin of Chinese Cherry Revealed by Chromosomal Karyotype and In Situ Hybridization of Seedling Progenies

Wang,

Li,

Feng

et al. 2023

Plants

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Genomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH

et al. 2021

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Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Silva

Souza

2020

Plant Biol J

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Passiflora edulis f. flavicarpa is the most economically important species in the genus Passiflora. However, the origin of this yellow form of passion fruit remains unclear, being suggested as a hybrid (P. edulis f. edulis × P. ligularis) or wild mutant. Here, the origin and genomic relationships of P. edulis f. flavicarpa with some related species in the genus Passiflora (subgenera Decaloba and Passiflora) were investigated using genomic in situ hybridization (GISH). Genomic DNA of 18 species was used as probe, which was hybridized onto chromosomes of P. edulis f. flavicarpa. Of all genomic DNA probes tested, none allowed us to identify a specific chromosome set in P. edulis f. flavicarpa. Conversely, probes from the subgenus Passiflora, P. edulis f. edulis, P. alata, P. cincinnata, P. coccinea, P. nitida and P. vitifolia, produced intense and uniform hybridizations on all chromosomes of P. edulis f. flavicarpa. Moreover, probes from P. ligularis, P. foetida and P. sublanceolata produced more intense hybridizations in the terminal region of four chromosomes, corresponding to the DNAr 45S locus, and also dispersed, less intense, hybridization across all chromosomes. Probes from the subgenus Decaloba, P. biflora, P. capsularis, P. cervii, P. coriacea, P. micropetala, P. morifolia, P. rubra and P. suberosa, produced hybridizations restricted to the DNAr 45S sites. The hybrid origin of P. edulis f. flavicarpa could not be supported based on the GISH results, and it is suggested that this species is conspecific with P. edulis f. edulis, because the probe with DNA of this form hybridized strongly throughout the target genome. The other putative parent species, P. ligularis, showed only a distant relationship with the target genome. The results also suggest that species of the subgenus Passiflora share many repetitive sequences and that the relationship between subgenera Decaloba and Passiflora is very distant.

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Genomicin situhybridization analysis betweenRubus coreanusand its relatives inRubus(Sect.Idaeobatus)

Cited by 4 publications

References 33 publications

Autotetraploid Origin of Chinese Cherry Revealed by Chromosomal Karyotype and In Situ Hybridization of Seedling Progenies

Autotetraploid Origin of Chinese Cherry Revealed by Chromosomal Karyotype and In Situ Hybridization of Seedling Progenies

Genomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

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