Genomic identification and characterization of co-occurring Harveyi clade species following a vibriosis outbreak in Pacific white shrimp, Penaeus (litopenaeus) vannamei

Bachand, Paxton T.; Tallman, James J.; Powers, Nicole C.; Woods, Megan A.; Azadani, Danial Nasr; Zimba, Paul V.; Turner, John

doi:10.1016/j.aquaculture.2019.734628

Cited by 10 publications

(7 citation statements)

References 62 publications

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“…Temperatures around 25 C favour Pdd growth and enhance the expression of virulence-related functions, thus contributing to the onset of outbreaks (Matanza and Osorio, 2018). Recent reports have raised the alarm about the isolation of this pathogen as a causative agent of disease in shrimp farms in far-off areas around the globe (Aguilera-Rivera et al, 2019;Sumithra et al, 2019;Bachand et al, 2020;Singaravel et al, 2020;Wang et al, 2020). Pdd can also infect humans through wounds exposed to the marine environment or inflicted while handling fish, tools and other objects in contact with seawater, and causes a very aggressive type of necrotising fasciitis with an elevated risk of complication into a fulminant death (Clarridge and Zighelboim-Daum, 1985;Yamane et al, 2004;Alhemairi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Withdrawn: The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

2022

Environ Microbiol Rep

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The marine bacterium Photobacterium damselae subsp. damselae (Pdd) causes disease in marine animals and humans. Previous studies demonstrated that mutation of the two-component system RstAB strongly impacts virulence of this pathogen, but the RstAB regulon has not been thoroughly elucidated. We here compared the transcriptomes of Pdd RM-71 and ΔrstA and ΔrstB derivatives using RNA-seq. In accordance with previous studies, RstAB positively regulated cytotoxins Dly, PhlyP and PhlyC. This analysis also demonstrated a positive regulation of outer membrane proteins, resistance against antimicrobials and potential virulence factors by this system. Remarkably, RstAB positively regulated two hitherto uncharacterised gene clusters involved in the synthesis of a polysaccharide capsule. Presence of a capsular layer in wild-type cells was confirmed by transmission electron microscopy, whereas rstA and rstB mutants were non-capsulated. Mutants for capsule synthesis genes, wza and wzc exhibited acapsular phenotypes, were impaired in resistance against the bactericidal action of fish serum and mucus, and were strongly impaired in virulence for fish, indicating a major role of capsule in virulence.Collectively, this study demonstrates that RstAB is a major positive regulator of key virulence factors including a polysaccharide capsule essential for full virulence in a pathogenic Photobacterium.

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Section: Introductionmentioning

confidence: 99%

Withdrawn: The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

2022

Environ Microbiol Rep

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“…Shrimp farming is an important and rapidly growing food production sector. However, disease caused by bacterial infections remains a limitation to the industry (Bachand et al, 2019). Among the bacterial diseases, vibriosis caused by various vibrios is causing tremendous economic losses and is responsible for massive mortality of cultured shrimp (Novriadi, 2016).…”

Section: Introductionmentioning

confidence: 99%

Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade

Zhang

Yang

Defoirdt

2021

J of Applied Microbiology

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Aim: Indole is a signaling molecule secreted by over 85 species of bacteria, including several Vibrio species, and it has been reported to affect different bacterial phenotypes such as biofilm formation, motility, and virulence. In this study, we aimed at investigating the inter-strain variability of the effect of indole in 12 different strains belonging to the Harveyi clade of vibrios. Methods and Results:Indole reduced the virulence of all strains towards gnotobiotic brine shrimp larvae. The survival rate of brine shrimp larvae challenged with vibrios pretreated with indole was increased by 1.3-fold to 1.8-fold. Additionally, indole significantly decreased the biofilm formation in all of the strains, decreased the swimming motility in eight of the strains, and decreased swarming motility in five of the strains. When cultured in the presence of exogenous indole, the mRNA level of the pirA and pirB toxin genes were down-regulated to 65% and 46%, and to 62% and 55% in the AHPND-causing strains Vibrio parahaemolyticus M0904 and Vibrio campbellii S01, respectively. Conclusions: These data indicate that indole has a significant impact on the virulence of different strains belonging to the Harveyi clade of vibrios. Significance and Impact of the Study: Our results suggest that indole signaling is a valid target for the development of novel therapeutics in order to control infections caused by Harveyi clade vibrios in aquaculture. K E Y W O R D S acute hepatopancreatic necrosis disease (AHPND), biofilm, early mortality syndrome (EMS), motility, quorum sensing How to cite this article: Zhang, S., Yang, Q. & Defoirdt, T. (2022) Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade.

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“…Citrobacter on the other hand had recently been identified from a freshwater crayfish gut after feeding with insect meal where the species is positively correlated to immunity (Foysal, Momtaz, et al., 2019). Compared to other genera, Photobacterium plays role in vibriosis of P. monodon (Aguilera‐Rivera et al., 2019) and P. vannamei (Bachand et al., 2020) with multiple co‐occurring Vibrio species wherein mutated P. damselae identified as global transporter of pathogenic Vibrio species (Bachand et al., 2020). In the control group, significantly abundant Aeromonas , Serratia and Flavobacterium have strong association with several diseases of aquatic animals (Foysal, Momtaz, et al., 2019; Janda & Abbott, 2010; McIntosh & Austin, 1990; Rahman et al., 2010).…”

Section: Discussionmentioning

confidence: 99%

Amplicon sequencing reveals significantly increased Vibrio abundance and associated gene functions in vibriosis‐infected black tiger shrimp (Penaeus monodon)

Foysal

Momtaz

Kawser

et al. 2020

Journal of Fish Diseases

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Vibriosis caused by luminous Vibrio species is one of the biggest challenges to shrimp industry in Bangladesh. This study aimed to characterize whole microbial communities from Vibrio‐infected black tiger shrimp (Penaeus monodon) using 16S rRNA‐based amplicon sequencing. A total of 36 disease‐free and infected shrimp were collected from six different hatcheries in Bagerhat, Bangladesh. A final pool of 12 samples (n = 6) was created by homogenization of the hepatopancreas samples from three shrimps collected from each hatchery for the same group. The amplicon sequencing data revealed significant (p < .05) decrease of alpha diversity measurements and subsequent effects (p < .05) on the hepatopancreas microbiota in the infected group, compared to control shrimp. Proteobateria and Aeromonas were the most dominant bacteria at phylum and genus level in both groups and identified as core microbiota in the community. Two bacterial groups at phyla level and eight at genus level were found associated with the alteration of hepatopancreas microbial communities and associated gene functions in vibriosis‐infected shrimp, revealed by differential abundance and KEGG pathway analysis. The overwhelming abundance of Citroibacter, Shewanella and Candidatus lineages in vibriosis‐infected shrimp needs further investigations.

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Genomic identification and characterization of co-occurring Harveyi clade species following a vibriosis outbreak in Pacific white shrimp, Penaeus (litopenaeus) vannamei

Cited by 10 publications

References 62 publications

Withdrawn: The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

Withdrawn: The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade

Amplicon sequencing reveals significantly increased Vibrio abundance and associated gene functions in vibriosis‐infected black tiger shrimp (Penaeus monodon)

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