Genomic in situ hybridization (GISH) and RFLP analysis for the identification of alien chromosomes in the backcross progeny of potato (+) tomato fusion hybrids

Abstract

Backcross progenies, BC1 and BC2, derived from crossing a hexaploid potato (+) tomato fusion hybrid to tetraploid potato, were monitored for the presence of alien tomato chromosomes. For discriminating the tomato chromosomes from those of potato, both mitotic and meiotic chromosome preparations were labelled differentially through total genomic DNA in situ hybridization (GISH). The presence or absence of individual tomato chromosomes in the BC progeny was identified through RFLP analyses using chromosome-specific DNA probes. The results indicated that the BC 1 plant possessed only nine chromosomes of tomato instead of the expected haploid set of 12. Of the nine persistent tomato chromosomes, there were three pairs that formed bivalents and three singles that formed univalents at meiosis. One of the single chromosomes was strikingly longer as a result of some structural mutation and could easily be identified in the hexaploid parent, the BC1 and in some of the BC2 plants. From the analyses of BC2 progeny there were indications of the presence in the BC1 of tomato chromosomes 1, 3 and 6 in duplicate, and chromosomes 8, 9 and 10 in the haploid condition. The number of tomato chromosomes transmitted to BC2 plants varied from one to six. The results of GISH revealed that a few BC2 plants possessed more tomato chromosomes than those deduced from RFLP analysis. This indicated the presence of some tomato chromosomes in duplicate and others in the haploid condition. The potential value of these genotypes for establishing potato lines with additions and substitutions of tomato chromosomes is discussed.

Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids

“…GISH has been useful to discriminate potato chromosomes from those belonging to species from other Solanum sections like tomato (Jacobsen et al 1995;Garriga-Calderé et al 1997, 1998 or S. nigrum (Horsman et al 2001). Within section Petota, GISH has been successfully used for hybrids between S. tuberosum Group Tuberosum and non-tuber bearing potato relatives carrying the E genome (Matsubayashi 1991), like S. brevidens (Dong et al 2001;Gavrilenko et al 2002;Tek et al 2004;Dong et al 2005) or S. etuberosum (Dong et al 1999;Gavrilenko et al 2003).…”

“…When GISH was combined with chromosome-specific molecular markers the transmission and segregation of individual chromosomes could be traced in potato (?) tomato fusion hybrids (Jacobsen et al 1995;Garriga-Calderé et al 1997, 1998 and wide potato hybrids (Ono et al 2016). This approach has also been used to elucidate the genomic constitution of wild allopolyploid species (Pendinen et al 2008(Pendinen et al , 2012.…”

Production of alien chromosome additions and their utility in plant genetics

“…1a and b. Reports on establishing the number of alien chromosomes in intergeneric backcross families are numerous (Raina and Rani, 2001), like tomato to potato (Jacobsen et al, 1995), maize to oat (Riera-Lizarazu et al, 1996), Beta corolliflora in beet (Gao et al, 2001), Solanum brevidens to potato (Dong et al, 2001), and S-genome chromosomes in wheat (Belyayev et al, 2001). The technique is also considered effective in studying individual chromosomes in plants (Schubert et al, 2001).…”

A study of the process of synchronisation and micronucleation in Beta vulgaris and the monitoring of an isolation procedure for micro-nuclei and micro-protoplasts by confocal laser scanning microscopy and flow cytometry

“…disease or stress resistance genes or genes controlling quality traits such as apomixis. Inter-specific or intergeneric transfer of specific chromosomes across sexual barriers and the production of addition and substitution lines could speed up introgressive hybridization (Sybenga 1992;Jacobsen et al 1995). In view of recent developments in the field of mutagenesis and recombination (Ispas G, Famelaer I and Angenon G, patent application W2006032504; Li et al 2006), MMCT would be a useful technology for the rapid introgression of foreign traits, as it avoids recurrent backcrosses or solves impossible hybridizations.…”