Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

Nakazawa, Daisuke; Kishimoto, Tomonari; Sato, Tomoi; Saito, Tomoka; Amano, Junji; Kuwayama, Sachiko; Okuno, Hajime; Godo, Toshinari; Watanabe, Yusuke; Han, Dongsheng; Nakano, Masaru

doi:10.1007/s10681-011-0393-2

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…6 Morphological characteristics of the plants (a), spikes (b), and seeds (c) of DM45 and its common wheat parent 7182 identify the chromosomal configuration and composition after crossing common wheat and its relatives. GISH is an accurate and efficient technique for this purpose (Nakazawa et al 2011). In the present study, GISH analysis using P. huashanica as a probe showed that the progeny DM45 was a wheat-L. mollis disomic substitution line (Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cytogenetic and Morphological Identification of a Wheat–L. mollis 1Ns(1D) Substitution Line, DM45

et al. 2016

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Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many potentially valuable traits for wheat breeding, i.e., strong and short stems, long spikes with numerous spikelets, tolerance to drought and cold, and resistance to many fungal and bacterial diseases. In this study, we hybridized a wheat-L. mollis triple substitution line 05DM6 × Triticum aestivum L. cv. 7182 to obtain DM45, a single chromosome substitution line. Cytological studies demonstrated that DM45 had a chromosome karyotype of 2n = 42 = 21II. Genomic in situ hybridization analysis indicated that DM45 had a pair of Ns chromosomes from L. mollis. Analysis with DNA markers, i.e., two simple sequence repeats (Xgdm111 and Xgdm126) and two expressed sequence tag-sequence tagged sites (CD453004 and BE443796), showed that the wheat 1Ds chromosome were substituted with a pair of 1Ns chromosomes from L. mollis in DM45. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis suggested that DM45 possessed Ns genomespecific bands in the low and high molecular weight glutenin subunit regions, whereas it lacked one glutenin subunit translated from genes on chromosome 1D, thereby confirming that DM45 was a wheat-L. mollis 1Ns#1 (1D) disomic substitution line. Agronomic trait evaluations showed that DM45 was much improved in terms of the 1000-grain weight and the protein and glutenin contents of its seeds, as well as having more florets and spikelets compared with its relative, common wheat variety 7182. The substitution line DM45 could be used as a novel germplasm in wheat genetic and breeding programs.

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Section: Discussionmentioning

confidence: 99%

Molecular Cytogenetic and Morphological Identification of a Wheat–L. mollis 1Ns(1D) Substitution Line, DM45

et al. 2016

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“…The use of GISH combined with classical cytogenetics to study the intergeneric somatic hybrid x Brassicoraphanus (2n = 2x = 38), derived from the cross between Brassica campestris ssp pekinensis (2n = 2x = 20) and Raphanus sativus (2n = 2x = 18), clearly identified that in the hybrid, 20 chromosomes were from Brassica and 18 chromosomes were from Raphanus (Lim et al, 2012). GISH analysis of the new cultivars from the cross between Littonia modesta (2n = 2x = 22), Sandersonia aurantiaca (2n = 2x = 24) and 3 cultivars belonging to the genus Gloriosa, G. superba 'Lutea' (2n = 2x = 22), G. superba 'Marrom Gold' (2n = 4x = 44), and G. superba 'Verschild' (2n = 7x = 77) demonstrated that in the 9 hybrids from intergeneric crosses involving the 3 genera, the genomes of the genitors were distinctly identified and there was no cross-hybridization, suggesting that the degree of homology between these 3 genera is relatively low, and that these genera diverged in the early evolutionary process (Nakazawa et al, 2011). …”

Section: Intergeneric Hybridsmentioning

confidence: 99%

Review Genomic in situ hybridization in plants

Silva¹,

Souza²

2013

Genet. Mol. Res.

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ABSTRACT. Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool has been applied to the study of hybrid lineages, genetic improvement programs, and studies of the evolution of polyploids. Moreover, GISH can be applied to the analysis of the meiotic behavior in hybrids and polyploids, providing information concerning the relationship between species. This review presents the wide application of this technique in plants.

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“…Genome constitution visualization and chromosome discrimination from different genomes in allopolyploids are carried out by GISH practices [15]. …”

Section: Introductionmentioning

confidence: 99%

Application of Genomic In Situ Hybridization in Horticultural Science

Ramzan

Younis

Lim

2017

International Journal of Genomics

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Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.

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Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

Cited by 10 publications

References 22 publications

Molecular Cytogenetic and Morphological Identification of a Wheat–L. mollis 1Ns(1D) Substitution Line, DM45

Molecular Cytogenetic and Morphological Identification of a Wheat–L. mollis 1Ns(1D) Substitution Line, DM45

Review Genomic in situ hybridization in plants

Application of Genomic In Situ Hybridization in Horticultural Science

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