Genomic insight into domestication of rubber tree

Chao, Jinquan; Wu, Shan; Shi, Minjing; Xu, Xiaojun; Gao, Qiang; Du, Huilong; Gao, Bin; Guo, Dong; Yang, Shuguang; Zhang, Shixin; Li, Yan; Fan, Xiuli; Hai, Chunyan; Kou, Liquan; Zhang, Jiao; Wang, Zhiwei; Liu, Yan; Xue, Wenbo; Xu, Jiang; Deng, Xianwen; Huang, Xinyi; Gao, Xinsheng; Zhang, Xiaofei; Hu, Yanshi; Zeng, Xia; Li, Weiguo; Zhang, Liangsheng; Peng, Shi-Qing; Wu, Jang‐Yen; Hao, Bingtao; Wang, Xuchu; Yu, Hong; Li, Jiayang; Liang, Chengzhi; Tian, Weimin

doi:10.1038/s41467-023-40304-y

Cited by 24 publications

(10 citation statements)

References 69 publications

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“…Exogenous PSK promoted the differentiation of laticifers in the epicormic shoots of rubber tree( Chao et al., 2023 ). While there are limited reports on the relationship between seed germination and rubber synthesis, our study revealed differential expression of genes related to rubber biosynthesis in specific tissues and at specific seed germination stages.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analysis reveals the molecular mechanisms of rubber biosynthesis and laticifer differentiation during rubber seed germination

Hu,

Yang,

Zhou

et al. 2024

Front. Plant Sci.

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The molecular mechanisms underlying the initiation of natural rubber synthesis and laticifer differentiation have not been fully elucidated. In this study, we conducted a time-series transcriptome analysis of five rubber tree tissues at four stages of seed germination. A total of 161,199 DEGs were identified between the two groups, including most 16,673 DEGs (A3 vs B3 and A3 vs C3) and lest 1,210 DEGs (C2 vs D2). We found that the maturation of the seed is accompanied by the formation of laticifer cells in cotyledon. Meanwhile, the analysis of hormones related genes expression may provide effective clues for us to promote the differentiation of laticifer cells in seeds by hormones in the future. In this study, hormone-related gene enrichment analyses revealed that IAA, GA, and CTK were activated in laticifer containing tissues. Similarly, GO and GEGG analysis showed that hormone pathways, especially the auxin pathway, are enriched. Gene expression clustering was analyzed using the short time-series expression miner (STEM), and the analysis revealed four distinct trends in the gene expression profiles. Moreover, we enriched transcription factor (TF) enrichment in cotyledon and embryonic axis tissues, and the MYB type exhibited the most significant difference. Furthermore, our findings revealed that genes related to rubber synthesis exhibited tissue-specific expression patterns during seed germination. Notably, key genes associated with rubber biosynthesis, specifically small rubber particle protein (SRPP) and cis-prenyltransferase (CPT), exhibited significant changes in expression in cotyledon and embryonic axis tissues, suggesting synchronous rubber synthesis with seed germination. Our staining results reveled that laticifer cells were exits in the cotyledon before seed imbibition stage. In conclusion, these results lay the foundation for exploring the molecular mechanisms underlying laticifer differentiation and rubber synthesis during seed germination, deepening our understanding of the initiation stages of rubber biosynthesis and laticifer differentiation.

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Section: Discussionmentioning

confidence: 99%

“…As the primary site of latex synthesis, the number of laticifers plays a critical role in rubber production. Recent research has shown that HbMYC26 and HbPSK5 (phytosulfokines) are positive regulatory factors of laticifer numbers ( Chao et al., 2023 ), suggesting that HbMYC26 and HbPSK5 may be involved in laticifer differentiation.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the molecular mechanisms of rubber biosynthesis and laticifer differentiation during rubber seed germination

Hu,

Yang,

Zhou

et al. 2024

Front. Plant Sci.

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“…In Hevea brasiliensis, the MYC transcription factors, as a core component of the JA signal, bind to the G-box motif of the HbPSK5 promoter and activate the HbPSK5 promoter. Overexpression of HbMYC26 and HbPSK5 in Taraxacum kok-saghyz could promote the formation of latex vessels and increase rubber content [50]. In Taraxacum brevicorniculatum, TbbZIP.1 binds to the ABRE elements of the TbSRPP promoter and regulates the expression of the TbSRPP in an ABA-dependent manner, increasing the latex content [51].…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of the HbREF1 Promoter from Hevea brasiliensis and Its Response to Phytohormones

Chen,

Guo,

Zhu

et al. 2024

Forests

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The rubber elongation factor (REF) is the most abundant protein in the latex of Hevea brasiliensis, which is closely related to natural rubber biosynthesis. In order to gain a deeper understanding of the transcriptional regulation mechanism of HbREF1, a 1758 bp genomic DNA fragment of the HbREF1 promoter was isolated. Promoter sequence analysis revealed several transcription factor binding sites in the HbREF1 promoter, such as bZIP, bHLH, EIL, AP2/ERF, MYB, and Trihelix. To assess the promoter activity, a series of HbREF1 promoter deletion derivatives were created and fused with firefly luciferase (LUC). The LUC image demonstrated that all of the HbREF1 promoters exhibited transcriptional activity. Furthermore, the assay revealed the presence of multiple regulatory elements within the promoter region that negatively regulate the transcriptional activity. Subsequent analysis of the transcriptional activity following treatment with phytohormones identified an ABA-responsive element located between −583 bp and −200 bp, an ET-responsive element between −718 bp and −583 bp, a JA-responsive element between −1758 bp and −1300 bp, and a SA-responsive element between −1300 bp and −718 bp. These results were largely consistent with the predictions of cis-acting elements. This study has established significant groundwork for future investigations into the regulatory mechanism of HbREF1.

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“…Comparative analysis of the intragenomic homeologous synteny provided evidence for the presence of paleotetraploidy in the species. Chao et al [ 73 ] demonstrated the relationship of increase in rubber yields during the domestication process with the increase in the number of laticifer rings and its high correlation with HbPSK5 encoding the small-peptide hormone phytosulfokine—a key domestication gene of rubber. Thus, through genomic studies, our understanding of the expression of different traits of agronomic interest in rubber trees has increased.…”

Section: Industrial Cropsmentioning

confidence: 99%