Genomic Instability:Signaling Pathways Orchestrating the Responsesto Ionizing Radiation and Cisplatin

Sakamoto-Hojo, Elza Tiemi; Mello, Stephano S.; Bassi, C.; Merchi, Igor M.; Carminati, P. O.; Fachin, Ana Lúcia; Donadi, Eduardo Antônio; Passos, Geraldo Aleixo Silva

doi:10.1007/7050_010

Cited by 4 publications

(3 citation statements)

References 164 publications

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“…However, only in the last decade has the concept of a coordinated DNA damage response integrating the processes underlying damage recognition, cell cycle checkpoints and DNA repair begun to be elucidated (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profiles in Human Lymphocytes Irradiated In Vitro with Low Doses of Gamma Rays

Fachin¹,

Mello²,

Sandrin-Garcia³

et al. 2007

Radiation Research

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The molecular mechanisms underlying responses to low radiation doses are still unknown, especially in normal lymphocytes, despite the evidence suggesting specific changes that may characterize cellular responses. Our purpose was to analyze gene expression profiles by DNA microarrays in human lymphocytes after in vitro irradiation (10, 25 and 50 cGy) with gamma rays. A cytogenetic analysis was also carried out for different radiation doses. G 0 lymphocytes were irradiated and induced to proliferate for 48 h; then RNA samples were collected for gene expression analysis. ANOVA was applied to data obtained in four experiments with four healthy donors, followed by SAM analysis and hierarchical clustering. For 10, 25 and 50 cGy, the numbers of significantly (FDR or=10 cGy (total aberrations) and >or=50 cGy (dicentrics/ rings). Therefore, low to moderate radiation doses induced qualitative and/or quantitative differences and similarities in transcript profiles, reflecting the type and extent of DNA lesions. The main biological processes associated with modulated genes were metabolism, stress response/DNA repair, cell growth/differentiation, and transcription regulation. The results indicate a potential risk to humans regarding the development of genetic instability and acquired diseases.

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Section: Introductionmentioning

confidence: 99%

Gene Expression Profiles in Human Lymphocytes Irradiated In Vitro with Low Doses of Gamma Rays

Fachin¹,

Mello²,

Sandrin-Garcia³

et al. 2007

Radiation Research

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show abstract

“…As RI também atuam nos checkpoints em G1/S, intra-S e G2/M, poucas horas após a exposição (Kuerbitz et al, 1992;Han et al, 1995;Bartek et al, 2004;Sakamoto-Hojo et al, 2005). A ativação desses checkpoints atrasa transitoriamente a progressão no ciclo de células danificadas pela radiação.…”

Section: Mecanismos De Indução De Danos No Dna Pelas Radiações Ionizaunclassified

Efeitos do Silenciamento de E2F1 e HEB, Fatores de Transcrição Preditos In Silico, em Células de Glioblastoma Irradiadas com Raios Gama.

Godoy¹

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“…Além dos citados produtos gênicos, foram ainda analisadas as proteínas As respostas celulares às radiações ionizantes são complexas e reguladas por diversas vias moleculares (Amundson et al, 2001;Khanna et al, 2001;Sakamoto-Hojo et al, 2006). Estudos realizados em células imortalizadas de camundongos revelaram que as respostas celulares à radiação dependem também da taxa de dose, sendo que as respostas variaram dependeram das taxas de dose, que variaram de 0,1 a 1,0 cGy/h e 5 cGy/h (Sugihara et al, 2004), mas existem poucos dados que realmente demonstram os efeitos da TD ao nível de respostas transcricionais.…”

Section: Expressão Protéicaunclassified

Modulação da expressão de genes de reparo do DNA em células humanas irradiadas com raios gama sob diferentes taxas de dose

Merchi¹

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Genomic Instability:Signaling Pathways Orchestrating the Responsesto Ionizing Radiation and Cisplatin

Cited by 4 publications

References 164 publications

Gene Expression Profiles in Human Lymphocytes Irradiated In Vitro with Low Doses of Gamma Rays

Gene Expression Profiles in Human Lymphocytes Irradiated In Vitro with Low Doses of Gamma Rays

Efeitos do Silenciamento de E2F1 e HEB, Fatores de Transcrição Preditos In Silico, em Células de Glioblastoma Irradiadas com Raios Gama.

Modulação da expressão de genes de reparo do DNA em células humanas irradiadas com raios gama sob diferentes taxas de dose

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