Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)

Yang, Moon-Sik; Wang, Kejian; Chen, Junhui; Qu, Huamin; Li, Shaojing

doi:10.1016/j.fsi.2007.04.011

Cited by 84 publications

(49 citation statements)

References 21 publications

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“…Cloning of hepcidin cDNA An AS-hepc2 (Acanthopagrus schlegelii B. hepcidin gene) cDNA 103 sequence of black porgy was reported previously (Yang et al, 2007) and that of red sea bream (PM-hepc) 104 was amplified in our laboratory and released on GenBank (GenBank accession no. AY557619).…”

Section: Porgy 102mentioning

confidence: 99%

Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP)

Wang

Yang

et al. 2009

Marine Environmental Research

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Hepcidin is known to be expressed in fish with bacterial challenge and iron overload. Here we first report the hepcidin expression induced in the developmental stages from embryo to fry of red sea bream (Pagarus major) and in juvenile black porgy (Acanthopagrus schlegelii B.) upon continuous waterborne exposure to BaP. The gene expression of CYP1A1 and IgL (immunoglobulin light chain) were both measured. Expression of the Pagarus major hepcidin gene (PM-hepc) was increased in post hatch fry at 24 h and 120 h exposure to BaP at concentrations of 0.1, 0.5 and 1.0 mu g/l, respectively. The gene expression pattern was comparable to that of CYP1A1 but different from that of IgL. In addition, a high number of AS-hepc2 transcripts (Acanthopagrus schlegelii B. hepcidin gene) were detected in the liver upon exposure to 1.0 mu g/l BaP. This study demonstrates that hepcidin gene expression is significantly induced in BaP-exposed red sea bream and black porgy. (C) 2008 Elsevier Ltd. All rights reserved.Fujian Science and Technology Department [20031005, 200710022]; National High Technology Research and Development Program of China (863 Program) [2007AA091406

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Section: Porgy 102mentioning

confidence: 99%

Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP)

Wang

Yang

et al. 2009

Marine Environmental Research

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“…The presence of hepcidin was detected in multiple tissues in few fish species such as in red seabream, Chrysophrys major (Chen et al 2005), zebrafish, Danio rerio (Shike et al 2002) and Atlantic salmon, Salmo salar (Douglas et al 2003). So far a number of isoforms of the hepcidins has been detected in fishes ranging from two (zebrafish, tetradon and seabreams) to ten (salmon) (Shike et al 2002;Yang et al 2007). The antimicrobial property of synthetic mature peptides of several fish hepcidins have also been studied against a broad range of Gram positive and negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of Antimicrobial Peptide, Hepcidin in Medium Carp, Puntius sarana

Das

Mohapatra

Sahoo

2014

Int J Pept Res Ther

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Antimicrobial peptides are considered as effector immune molecules and during last decades, many workers have demonstrated the significant role and mechanism of hepcidin (hamp), an antimicrobial peptide in regulating the absorption, transport, storage and mobilization of iron. In the present work, hepcidin cDNA was cloned, sequenced and characterized in an important medium carp species, Puntius sarana, which is enlisted as a vulnerable species. The tissue specificity of hepcidin was checked in major organs of this species, and expression profile of hepcidin transcript was analyzed during ontogeny and infection against a common bacterial pathogen, Aeromonas hydrophila. Further, the antimicrobial activity of hepcidin was investigated against various Gram positive and negative bacterial strains, using the synthetic mature peptide of P. sarana. The obtained hamp amplicon produced a predicted ORF of 279 bases having 92 amino acids and the nucleotide sequence showed 99 % identity with zebrafish hepcidin. During the ontogeny, the hamp gene appeared at 6 h postfertilization and its expression remained constant towards 21 days post-fertilization and was intensely observed in liver as compared to other organs. After artificial exposure to A. hydrophila, up-regulation of the gene was marked at 3 and 6 h post-challenge, however, the same was down regulated after 24 h onwards. The synthetic hepcidin peptide exhibited broad spectrum antibacterial activity against important fish pathogens at a concentration of 17.30 lM. This preliminary study revealed that hepcidin might be playing an important role in innate defence of this fish species.

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“…Because this taxonomic group is not thought to have undergone additional WGDs, the presence of multiple LEAP-2 subisoforms in this fish species might be due to the gene-specific duplication(s) during its adaptive evolution. Duplication and/or amplification of AMP genes in certain Eupercaria fish groups have been previously reported with a proposed explanation based on the positive Darwinian selection (i.e., an adaptive evolutionary process directed by pathogens when the host fish is exposed to new environments) (Padhi and Verghese 2007), as highlighted by diversified hepcidin (LEAP-1) isoforms and subisoforms in those fish species (Cho et al 2009;Yang et al 2007;Lee and Nam 2011). Currently, it is unclear whether or not mud loach possesses additional LEAP-2 isoform (i.e., LEAP-2C) and/or subisoforms.…”

Section: Resultsmentioning

confidence: 96%

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

Lee

Nam

2017

Fish Aquatic Sci

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Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5′-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion.

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Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)

Cited by 84 publications

References 21 publications

Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP)

Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP)

Cloning and Characterization of Antimicrobial Peptide, Hepcidin in Medium Carp, Puntius sarana

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

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