Genomic organization and transcription of the medaka and zebrafish cellular retinol-binding protein (rbp) genes

Parmar, Manoj B.; Shams, Rana Muhammad Athar Azeem; Wright, Jonathan

doi:10.1016/j.margen.2013.04.001

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…Their cDNA IDs, nucleotides and encoded amino acid numbers with theoretical p I s and MWs are presented in Table 3. The four exons interrupted by three introns were determined in all the cloned fabp genes, consistent with earlier studies reporting four exons and three introns for the fabp genes (Parmar et al, 2012; Parmar, Shams, & Wright, 2013; Venkatachalam et al ., 2017). Moreover, all identified S. aurata fabp genes followed the GT‐AG rule (Mount, 1982) (Data S1).…”

Section: Resultssupporting

confidence: 88%

Fatty acid–binding protein genes in gilthead seabream: molecular cloning and nutritional regulation under low water temperatures

Taşbozan

Erbaş

Bayır

et al. 2023

Journal of Fish Biology

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The molecular characteristics and tissue disruption of 10 fatty acid-binding protein (fabp) genes in gilthead seabream (Sparus aurata) were investigated, and their expression levels were found in the fish fed diets with different vegetable oil (VO) sources, which may explore the potential function of fabp genes in S. aurata. For this purpose, the open reading frames of fabp genes involved in the transport and ß-oxidation of fatty acids (FA) were molecularly cloned and characterized. S. aurata was then exposed to a two-staged feeding trial (the grow-out period following a wash-out period) at low water temperatures. In the grow-out period, the fish were fed diets containing 50% and 100% ratios of various VOs for 60 days, and in the wash-out period, the fish were fed a diet containing 100% fish oil (FO) for 30 days. It has been determined that (a) S. aurata and vertebrate fabp/FABP genes are orthologues;(b) spatio-temporal differences in tissue-specific patterns of fabp genes differ importantly; for instance, the difference between the highest and lowest values reaches 13 Â 10 5 -fold in the fabp10a; and (c) VO-based diets upregulated fabp transcript levels in the liver and muscle with some exceptions, such as liver fabp11a and muscle fabp7a. Gene expressions of only the hepatic fabp7b and fabp10a genes were diminished at the end of the wash-out period. In this study, the authors provide further evidence that dietary FAs affect fabp mRNA expressions in S. aurata. This might be useful in the nutritional control of fabp genes to maintain lipid homeostasis in marine fish fed VO-based diets at low water temperatures.

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Section: Resultssupporting

confidence: 88%

Fatty acid–binding protein genes in gilthead seabream: molecular cloning and nutritional regulation under low water temperatures

Taşbozan

Erbaş

Bayır

et al. 2023

Journal of Fish Biology

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“…D. labrax Fabp proteins consisted of 126–134 amino acids, and their MWs ranged from 14.01 kDa to 15.17 kDa, which are similar to previous studies that reported the iLBPs consisting of 125–146 amino acids and ~15 kDa MW (Bayır, 2021; Parmar et al, 2013a). All the identified fabp genes are highly conserved in their genomic organizations, consisting of four exons with comparable coding sizes and three introns of different sizes, which is in agreement with previous studies on teleost fish (Venkatachalam et al, 2012, 2017).…”

Section: Discussionsupporting

confidence: 87%

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Taşbozan

Erbaş

Bayır

et al. 2022

Aquaculture Research

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Vegetable oils (VOs) are preferred by the aquafeed industry as alternative oil sources to fish oil (FO) for sustainable aquaculture production because they decrease catching pressure for wild fish stocks, and compared with FO, VOs have higher annual production, easier accessibility and lower prices (Bayır et al., 2011). VOs are rich sources of 18C fatty acids (FAs), but their n-3 highly unsaturated

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“…Retinol-binding protein (RBP) is a carrier of vitamin A that is mainly synthesized and released in liver [60]. RBP binds retinol and retinal, and as such is thought to play an important role in vitamin A (retinol) uptake, transport and metabolism [61]. Up-regulation of RBP enhances uptake of vitamin A to enhance fish immunity and growth [62].…”

Section: Discussionmentioning

confidence: 99%

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

Wen

Zhang

et al. 2019

BMC Genomics

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Background T. fasciatus ( Takifugu fasciatus ) faces the same problem as most warm water fish: the water temperature falls far below the optimal growth temperature in winter, causing a massive death of T. fasciatus and large economic losses. Understanding of the cold-tolerance mechanisms of this species is still limited. Integrated application of multi-omics research can provide a wealth of information to help us improve our understanding of low-temperature tolerance in fish. Results To gain a comprehensive and unbiased molecular understanding of cold-tolerance in T. fasciatus , we characterized mRNA-seq and metabolomics of T. fasciatus livers using Illumina HiSeq 2500 and UHPLC-Q-TOF MS. We identified 2544 up-regulated and 2622 down-regulated genes in the liver of T. fasciatus . A total of 40 differential metabolites were identified, including 9 down-regulated and 31 up-regulated metabolites. In combination with previous studies on proteomics, we have established an mRNA-protein-metabolite interaction network. There are 17 DEMs (differentially-expressed metabolites) and 14 DEGs-DEPs (differentially co-expressed genes and proteins) in the interaction network that are mainly involved in fatty acids metabolism, membrane transport, signal transduction, and DNA damage and defense. We then validated a number of genes in the interaction network by qRT-PCR. Additionally, a number of SNPs (single nucleotide polymorphisms) were revealed through the transcriptome data. These results provide key information for further understanding of the molecular mechanisms of T. fasciatus under cold stress. Conclusion The data generated by integrated application of multi-omics can facilitate our understanding of the molecular mechanisms of fish response to low temperature stress. We have not only identified potential genes and SNPs involved in cold tolerance, but also show that some nutrient metabolites may be added to the diet to help the overwintering of T. fasciatus . Electronic supplementary material The online version of this article (10.1186/s12864-019-5915-7) contains supplementary material, which is available to authorized users.

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Genomic organization and transcription of the medaka and zebrafish cellular retinol-binding protein (rbp) genes

Cited by 9 publications

References 50 publications

Fatty acid–binding protein genes in gilthead seabream: molecular cloning and nutritional regulation under low water temperatures

Fatty acid–binding protein genes in gilthead seabream: molecular cloning and nutritional regulation under low water temperatures

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

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