2007
DOI: 10.1016/j.vetimm.2006.11.005
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Genomic organization of the T-cell receptor γ gene and PCR detection of its clonal rearrangement in canine T-cell lymphoma/leukemia

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Cited by 38 publications
(71 citation statements)
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“…9,15 Briefly, genomic DNA was extracted from PBMCs using a commercial extraction kit. g For detection of clonal rearrangement of the TCRc gene, 2 different primers for V segments (Vca: 59-CGTGTACTACTGCG-CTGCCTGG-39 and Vcb: 59-GGCTGTATTACTGTG-CCTGCTGG-39) and 2 different primers for J segments (Jca: 59-TACCTTCTG(C/T)AAATATCTTGA-39 and Jcb: 59-TGTGCCAGGACCAAGCACTTTGTT-39) were used.…”
Section: Clonality Analysismentioning
confidence: 99%
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“…9,15 Briefly, genomic DNA was extracted from PBMCs using a commercial extraction kit. g For detection of clonal rearrangement of the TCRc gene, 2 different primers for V segments (Vca: 59-CGTGTACTACTGCG-CTGCCTGG-39 and Vcb: 59-GGCTGTATTACTGTG-CCTGCTGG-39) and 2 different primers for J segments (Jca: 59-TACCTTCTG(C/T)AAATATCTTGA-39 and Jcb: 59-TGTGCCAGGACCAAGCACTTTGTT-39) were used.…”
Section: Clonality Analysismentioning
confidence: 99%
“…Clonality analysis using polymerase chain reaction (PCR) amplification for dogs has been previously reported. 4 More recently, a similar assay was developed based on the canine genome database by targeting the T-cell receptor gamma chain (TCRc) gene 15 and the immunoglobulin heavy chain (IgH) gene. 9 Although the diagnostic value of PCR analysis has been demonstrated in several types of lymphoproliferative disorders, 7,[11][12][13][14] only limited studies have been done to identify neoplastic proliferation of lymphocytes in dogs with small-cell lymphocytosis.…”
Section: Introductionmentioning
confidence: 99%
“…As a consequence, the immunohistochemical characterization of skin infiltrates is generally not useful to distinguish between neoplastic and nonneoplastic Tcell infiltrates, and the histologic detection of earlystage ETCL remains a big challenge in dermatopathology. 4,15 At present, only molecular biology approaches based on the analysis of T-cell receptor (TCR) genes can help to distinguish reactive T-cell infiltrates from T-cell lymphomas. 4,9,15 In dogs and other mammals, TCR is a complex gene that consists of a and b, or c and d chains.…”
Section: Introductionmentioning
confidence: 99%
“…4,15 At present, only molecular biology approaches based on the analysis of T-cell receptor (TCR) genes can help to distinguish reactive T-cell infiltrates from T-cell lymphomas. 4,9,15 In dogs and other mammals, TCR is a complex gene that consists of a and b, or c and d chains. The 4 chains are encoded by 4 distinct loci designated TCRa, TCRb, TCRc, and TCRd.…”
Section: Introductionmentioning
confidence: 99%
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