Genomic rearrangement between wheat and Thinopyrum elongatum revealed by mapped functional molecular markers

Hu, Lijun; Liu, Cheng; Zeng, Zixian; Guang-rong, LI; Song, Xiao-Jin; Yang, Zujun

doi:10.1007/s13258-011-0153-7

Cited by 38 publications

(35 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies showed that the PLUG markers were useful for producing alien chromosome-specific markers , Hu et al, 2012. A total of 12 PLUG markers from wheat homologous group 2 were tested on D11-5 compared to its parents MY11 and TDH-2 ( table 1 ).…”

Section: Molecular Marker Analysismentioning

confidence: 99%

A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrumbreviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V^b (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V^b were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V^b of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.

show abstract

Section: Molecular Marker Analysismentioning

confidence: 99%

A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…uniaristata , Th. intermedium [Li et al, 2013], and Lophopyrum elongatum [Hu et al, 2012]. However, markers specific for Ae.…”

Section: Discussionmentioning

confidence: 98%

“…The PLUG-PCR protocol followed Hu et al [2012]. In order to obtain high levels of polymorphisms, the PCR products were digested with the 4-base cutter enzymes Hae III and Taq I according to Ishikawa et al [2007] and were separated on 2% agarose gels.…”

Section: Dna Isolation and Est-based Pcrsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line

Liu

Li²,

Gong³

et al. 2015

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA)₈ as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing. EST-based molecular marker data suggested that the wheat-Ae. mutica addition line carries the 7T chromosome. Resistance tests indicated that both the partial amphiploid and the 7T addition line were highly resistant to powdery mildew, whereas the wheat control line Chinese Spring was highly susceptible, indicating the presence of a potentially new powdery mildew resistance gene on the Ae. mutica 7T chromosome. The karyotype, FISH patterns, and molecular markers can now be used to identify Ae. mutica chromatin in a wheat background, and the 7T addition could be used as a new powdery mildew resistance source for wheat breeding.

show abstract

“…In the earlier studies, the PLUG markers were used in wheat for identification chromatin of distant genetic species Thinopyrum elongatum [15], Th. intermedium [16], Leymus mollis [17], Th.…”

Section: Variety Testingmentioning

confidence: 99%

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

Sokolov¹,

Polkhovskiy²,

Kroupin³

et al. 2017

S-h. biol.

View full text Add to dashboard Cite

A b s t r a c t Molecular markers make it possible to monitor the directional introgression of alien genetic material to the genome of wheat. Among the diverse molecular markers, the PLUG (PCR-based landmark unique gene) markers have the greatest advantage for analyzing distant hybrids due to the ability to amplify polymorphism fragments on closely related species and to detect simultaneously three genomes within one homoeological group in one PCR. A collection of 41 entries of different breeding stages developed in Russian State Agrarian University as a result of crossing of bread wheat (Triticum aestivum L.) and wheat T. timopheevii Zhuk. was studied. All hybrids were resistant to leaf rust and powedery mildew natural infection. Using this collection of distant hybrids, we demonstrated for the first time the possibilities of PLUG markers for identifying alien introgressions in the genome of bread wheat from T. timopheevii. In order to increase the degree of polymorphism between the entries, we additionally processed the products of amplification of PLUG markers with highfrequency restriction endonucleases TaqI and HaeIII. The entries were found to carry both single and multiple introgressions. The latter entries with introgressions in more than one homeological group are of particular interest. The heterogeneity of not only the late generations of entries, but also the entries from the nursery of the preliminary cultivar estimation, which may be associated with secondary chasmogamy, is shown. As a result of the studies, 14 PLUG markers for the long and short chromosome arm of each homeological group have been selected, which can be recommended for the detection of the genetic material of T. timopheevii in distant hybrids of T. timopheevii ½ T. aestivum. The advantages of PLUG markers as a tool for monitoring the transfer of alien hereditary material into the genome of bread wheat are discussed.Keywords: common wheat, Triticum aestivum, Triticum timopheevii, wide hybridization, molecular markers, PLUG, PCR The predominance of bread wheat (Triticum aestivum L., A u BD) over other wheat species is determined by ecological plasticity, resistance to low and high temperatures, excess and shortage of moisture, diseases and pests. Currently, this is the main food crop for about one third of the world's population [1,2]. However, in connection with impoverishment of genetic resources of bread wheat, evolution of pathogens, loss of efficiency of the present disease and pest resistance genes, a problem of search for new resistance genes for transfer into the T. aestivum genome at distant hybridization becomes important.As a source of such genes, relatives of bread wheat are used, in particular,

show abstract

Genomic rearrangement between wheat and Thinopyrum elongatum revealed by mapped functional molecular markers

Cited by 38 publications

References 64 publications

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-<b><i>Aegilops mutica</i></b> Partial Amphiploid and Addition Line

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

Contact Info

Product

Resources

About