Genomic Sequence of Klebsiella pneumoniae IIEMP-3, a Vitamin B
            <sub>12</sub>
            -Producing Strain from Indonesian Tempeh

Yulandi, Adi; Sugiokto, Febri Gunawan; Febrilina,; Suwanto, Antonius

doi:10.1128/genomea.01724-15

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…Vitamin B12 in tempeh was naturally produced by bacteria, for example, K. pneumoniae and C. freundii (Areekul et al., 1990; Keuth & Bisping, 1993; Liem et al., 1977; Okada, 1989). The vitamin B12‐producing K. pneumoniae strains in tempeh were not pathogenic given that they did not produce enterotoxin and had different genetic profiles compared to those pathogenic to humans (Keuth & Bisping, 1993; Yulandi et al., 2016). In Indonesia, harmless Klebsiella spp.…”

Section: Resultsmentioning

confidence: 99%

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Ahnan-Winarno

Cordeiro

Winarno

et al. 2021

Comp Rev Food Sci Food Safe

147

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Tempeh is a fermented food made of mainly soybeans and is a nutritious, affordable, and sustainable functional source of protein. Globally, tempeh is a widely accepted fermented product. Although there is a growing body of literature on tempeh, most research has focused on unfermented soybeans, thus the impact of tempeh fermentation on biological properties of soybeans has been largely left scattered. The objective of this review is to summarize the literature of tempeh fermentation over the past 60 years. A search of articles on tempeh published from 1960 to 2020 was performed using the Cochrane Library, Web of Science, EBSCOhost FSTA database, and Google Scholar. References from identified articles were reviewed for additional sources. In total, 321 papers were selected for this review, of which 64 papers were related to the health benefits of tempeh. This review concluded that sufficient evidence exists in the literature supporting tempeh fermentation as a low‐cost, health‐promoting, and sustainable food processing technology to produce protein‐rich foods using various beans, legumes, and grains. This comprehensive review suggests further studies are needed on tempeh fermentation and its impact on human health; research and standardization of nonsoy tempeh; assessment of food safety‐improving modification in tempeh production system; and initiatives supporting the sourcing of local ingredients in tempeh production.

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Section: Resultsmentioning

confidence: 99%

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Ahnan-Winarno

Cordeiro

Winarno

et al. 2021

Comp Rev Food Sci Food Safe

147

View full text Add to dashboard Cite

show abstract

“…Some K. pneumoniae isolates from EMP and WJB tempeh fully sequenced and subjected to genomic comparison to pathogenic strain in the previous study [29]. K. pneumoniae is known to produce vitamin B 12 during tempeh fermentation [30]. Data analysis from the amplicon sequencing of metagenomic samples of EMP and WJB tempeh in the previous study [4,5] failed to reveal this important genus.…”

Section: Discussionmentioning

confidence: 99%

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

Yulandi

Suwanto²,

Waturangi

et al. 2020

Preprint

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Objective: Amplicon sequencing targeting 16S ribosomal RNA (rRNA) has been widely used to profile the microbial community from fermented food samples. However, polymerase chain reaction (PCR) steps on amplicon sequencing analysis and intragenomic heterogeneity within 16S rRNA are believed to contribute to bias in estimating microbial community composition. As potential paraprobiotics sources, a comprehensive profiling study of tempeh microbial ecology could contribute to tempeh product development. This study employed a shotgun metagenomic approach, where metagenome fragments from tempeh samples were sequenced directly for taxonomic and functional profiling analysis.Results: Taxonomic profiling showed that Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla from the shotgun metagenomic analysis in all tempeh samples. In terms of composition, this shotgun metagenomic study revealed that Proteobacteria was the most abundant phylum. Functional profiling showed that iron complex outer-membrane recepter protein (KEGG ID: K02014) was the most transcribed gene based on this metagenomic analysis. The metagenome-assembled genomes (MAGs) results from the binning pipeline could reveal almost complete whole genome sequence of Lactobacillus fermentum, Enterococcus cecorum, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii.

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“…Some K. pneumoniae isolates from EMP and WJB tempeh fully sequenced and subjected to genomic comparison to pathogenic strain in the previous study [25]. K. pneumoniae is known to produce vitamin B 12 during tempeh fermentation [26]. Data analysis from amplicon sequencing of metagenomic samples of EMP and WJB tempeh in the previous study [6,7] failed to reveal this important genus.…”

Section: Discussionmentioning

confidence: 99%

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

Yulandi

Suwanto

Waturangi

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Objective: Amplicon sequencing targeting 16S ribosomal RNA (rRNA) has been widely used for the profile analysis of the microbial community from fermented food samples. Previous results of 16S rRNA analysis metagenome showed that Firmicutes was the dominant phylum in tempeh. However, polymerase chain reaction (PCR) steps on amplicon sequencing analysis and intragenomic heterogeneity within 16S rRNA are believed to contribute to bias in the estimation of microbial community composition. An alternative approach known as shotgun metagenomic might be able to avoid this limitation. In this study, we employed total metagenomic DNA fragments that were sequenced directly for taxonomic dan functional profiling analysis.Results: Taxonomic profiling showed that Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla from the direct shotgun metagenomic analysis in all tempeh samples. In terms of composition, this shotgun metagenomic study revealed that Proteobacteria was the most abundant phylum. Functional profiling showed that iron complex outer-membrane recepter protein (KEGG ID: K02014) was the most transcribed genes based in this metagenomic analysis. The binning pipeline could reveal almost complete whole genome sequence of Lactobacillus fermentum, Enterococcus cecorum, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii.

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Genomic Sequence of Klebsiella pneumoniae IIEMP-3, a Vitamin B ₁₂ -Producing Strain from Indonesian Tempeh

Cited by 7 publications

References 11 publications

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

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Genomic Sequence of Klebsiella pneumoniae IIEMP-3, a Vitamin B 12 -Producing Strain from Indonesian Tempeh

Cited by 7 publications

References 11 publications

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

Shotgun Metagenomic Analysis Reveals New Insights into Bacterial Community Profiles in Tempeh

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Product

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Genomic Sequence of Klebsiella pneumoniae IIEMP-3, a Vitamin B ₁₂ -Producing Strain from Indonesian Tempeh