Genomic Sequencing of Bacillus cereus Sensu Lato Strains Isolated from Meat and Poultry Products in South Africa Enables Inter- and Intranational Surveillance and Source Tracking

Carroll, Laura M.; Pierneef, Rian; Mathole, Aletta; Atanda, Abimbola; Matle, Itumeleng

doi:10.1128/spectrum.00700-22

Cited by 9 publications

(8 citation statements)

References 112 publications

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“…Several extensively analyzed strains have been derived from diverse sources, such as fermented foods [ 62 ], meat and poultry products [ 63 ], and plants [ 64 ]. Investigations utilizing whole-genome sequencing techniques have unveiled strains of B. paranthracis exhibiting potential probiotic characteristics [ 64 ] and demonstrating biodegradation capabilities [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer

Baev,

Iliev,

Stefanov

et al. 2024

CIMB

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Phospholipases find versatile applications across industries, including detergent production, food modification, pharmaceuticals (especially in drug delivery systems), and cell signaling research. In this study, we present a strain of Bacillus paranthracis for the first time, demonstrating significant potential in the production of phosphatidylcholine-specific phospholipase C (PC-PLC). The investigation thoroughly examines the B. paranthracis PUMB_17 strain, focusing on the activity of PC-PLC and its purification process. Notably, the PUMB_17 strain displays extracellular PC-PLC production with high specific activity during the late exponential growth phase. To unravel the genetic makeup of PUMB_17, we employed nanopore-based whole-genome sequencing and subsequently conducted a detailed genome annotation. The genome comprises a solitary circular chromosome spanning 5,250,970 bp, featuring a guanine–cytosine ratio of 35.49. Additionally, two plasmids of sizes 64,250 bp and 5845 bp were identified. The annotation analysis reveals the presence of 5328 genes, encompassing 5186 protein-coding sequences, and 142 RNA genes, including 39 rRNAs, 103 tRNAs, and 5 ncRNAs. The aim of this study was to make a comprehensive genomic exploration that promises to enhance our understanding of the previously understudied and recently documented capabilities of Bacillus paranthracis and to shed light on a potential use of the strain in the industrial production of PC-PLC.

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Section: Resultsmentioning

confidence: 99%

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer

Baev,

Iliev,

Stefanov

et al. 2024

CIMB

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“…and collaborators isolated B. cereus group strains during routine surveillance of meat products ( 12 ). WGS was conducted on some strains ( 13 ). I.M.…”

Section: The Studymentioning

confidence: 99%

“…and L.M.C. assigned B. cereus group strains to species using multiple WGS-based methods ( 13 ); one method relied on the Genome Taxonomy Database (GTDB), a popular contemporary microbial species classification framework ( 14 ). GTDB releases R95 and R202 classified 2 strains as B. anthracis ( Table 1 ); however, neither strain belonged to the historical, clonal B. anthracis lineage ( 6 ), and neither possessed anthrax toxin- or capsule-encoding genes ( Table 1 ; Figure 1 ).…”

Section: The Studymentioning

confidence: 99%

Laboratory Misidentifications Resulting from Taxonomic Changes to Bacillus cereus Group Species, 2018–2022

Carroll¹,

Matle²,

Kovač³

et al. 2022

Emerg. Infect. Dis.

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“…A previous study indicated that some whole genome sequencing (WGS)-based species classification methods pertaining to the B. cereus group can be easily misinterpreted [ 2 ]. Current species definitions of the B. cereus group result in overlapping genomospecies clusters, with 66.2% of genomes belonging to multiple genomospecies at a conventional 95 average nucleotide identity (ANI) threshold, while a ≈92.5 ANI threshold reveals distinct genomospecies clusters with minimal overlap [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Bacillus paranthracis , first identified in 2017, was historically referred to as B. cereus or group III B. cereus and encompasses B. cereus group strains capable of causing emetic and/or diarrhoeal foodborne illness [ 3 ]. Here, we conducted a retrospective study on a water-borne outbreak caused by B. paranthracis and the contamination status of paranthracis in the surrounding water systems.…”

Section: Introductionmentioning

confidence: 99%

A rare waterborne outbreak of Bacillus paranthracis in Shandong province, China, 2020: epidemiologic survey, genomic insights, and virulence characteristics

Wang,

Xie,

Liu

et al. 2024

Emerging Microbes & Infections

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Bacillus paranthracis , a Gram-positive conditional pathogen of Bacillus cereus group species, is capable of causing foodborne and waterborne illnesses, leading to intestinal diseases in humans characterized by diarrhoea and vomiting. However, documented cases of B. paranthracis infection outbreaks are rare in the world, and the genomic background of outbreak strains is seldom characterized. This study retrospectively analyzed strains obtained from an outbreak in schools, as well as from water systems in peri-urban areas, China, in 2020. In total, 28 B. cereus group isolates were retrieved, comprising 6 from stool samples and 22 from water samples. Epidemiological and phylogenetic investigations indicated that the B. paranthracis isolate from drinking water as the causative agent of the outbreak. The genomic comparison revealed a high degree of consistency among 8 outbreak-related strains in terms of antimicrobial resistance gene profiles, virulence gene profiles, genomic content, and multilocus sequence typing (MLST). The strains related to the outbreak show highly similar genomic ring diagrams and close phylogenetic relationships. Additionally, this study shed light on the pathogenic potential and complexity of B. cereus group through its diversity in virulence genes and mice infection model. The findings highlight the usefulness of B. paranthracis genomes in understanding genetic diversity within specific environments and in tracing the source of pathogens during outbreak situations, thereby enabling targeted infection control interventions.

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Genomic Sequencing of Bacillus cereus Sensu Lato Strains Isolated from Meat and Poultry Products in South Africa Enables Inter- and Intranational Surveillance and Source Tracking

Abstract: Nationwide foodborne pathogen surveillance programs that use high-resolution genomic methods have been shown to provide vast public health and economic benefits. However, Bacillus cereus sensu lato is often overlooked during large-scale routine WGS efforts.

Cited by 9 publications

References 112 publications

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer

Laboratory Misidentifications Resulting from Taxonomic Changes to Bacillus cereus Group Species, 2018–2022

A rare waterborne outbreak of Bacillus paranthracis in Shandong province, China, 2020: epidemiologic survey, genomic insights, and virulence characteristics

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