Genomic structure and chromosome location of RPL27A/<i>Rpl27a,</i> the genes encoding human and mouse ribosomal protein L27A

Abstract

The intron-containing genes encoding human and mouse ribosomal protein (r-protein) L27A were cloned and sequenced. The human r-protein L27A gene (RPL27A) shared an identical exon/intron structure with the mouse r-protein 27A gene (Rpl27a). The translational start codon ATG was separated from the main reading frame by the first intron sequence in both genes. An approximately 200-bp sequence upstream of the translational start site of both genes displayed remarkable similarity, and contained the putative promoters lacking canonical TATA, but harbored Sp1 binding sites and a short stretch of pyrimidine cluster, similar to other r-protein genes. Transcriptional regulatory elements, Box-A and GABP, found in the promoters of some other r-protein genes were also conserved in both genes. These structural features were included in the typical CpG island identified in the 5′-end sequences, suggesting that RPL27A/Rpl27a cloned here are authentic and transcriptionally active. Fluorescence in situ hybridization (FISH) analysis localized the mouse intron-containing Rpl27a to chromosome 7E2–F1 syntenic to human chromosome 11p15, where human RPL27A was located.

Rpl27a mutation in the sooty foot ataxia mouse phenocopies high p53 mouse models

“…Fine mapping in this interval identified a region that included 12 odorant receptor genes which we did not consider as candidates, and 13 additional genes with at least 120 exons that were subjected to sequence analysis in C57BL/6J and mice heterozygous for the SFA mutation ( SFA/ + mice) (Figure 1A). The scan for mutations identified an A > G point mutation at position − 15 in the 3′ splice acceptor of intron 4 (IVS4‐15A > G) within the Rpl27a gene, which is a large subunit ribosomal protein of 148 amino acids encoded by five exons 29 (Figure 1B). This mutation was the only one found in 82 kb of sequenced genomic DNA.…”

Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle

“…) and in structuring of ribosomes (Kusuda et al . ), respectively. We showed that the c.*188G>A SNP in 3′ untranslated region of AKIRIN2 and the g.3109537C>T SNP in 5′ flanking region of RPL27A were associated with BMS number also in Oita prefecture (Sasaki et al .…”

Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene

“…Similarly, the N site in the ¢rst exon of X. laevis L1 and L14 r-protein genes was shown to play a marginal role in transient expression of a reporter gene [30]. A region (TCGCGAGA) at position 320 is identical in sequence and position to the Box-Alike element described in the human and mouse L27 r-protein genes [31] and similar to the Box-A of the mouse L7a r-protein which has been shown to bind nuclear factors [32]. Finally, two inverted GABP binding sites were found around the transcription start site, which are identical in sequence and position to those found in the mouse S16 r-protein and cytochrome c oxidase CO4 and CO5b genes.…”