Genomic structure and expression of parathyroid hormone-related protein gene (PTHrP) in a teleost, Fugu rubripes

Power, Deborah M.; Ingleton, P. M.; Flanagan, Julia; Canário, Adelino V.M.; Danks, Janine A.; Elgar, Greg; Clark, Melody S.

doi:10.1016/s0378-1119(00)00167-0

Cited by 52 publications

(38 citation statements)

References 33 publications

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“…The principal hypercalcaemic factor identified in tetrapods, parathyroid hormone (PTH), has not been identified in fish. However, both puffer fish and the sea bream (Flanagan et al 2000) have recently been shown to express the gene for PTH-related protein (PTHrP), and a peptide corresponding to the amino acid residues 1-38 of the N-terminal region of PTHrP was able to cause an elevation in whole body calcium uptake in sea bream larvae, by increasing uptake and decreasing efflux of calcium (Guerreiro et al 2001). Although the involvement of PTHrP in the hypercalcaemic effect of E 2 in the present study was beyond the experimental objectives, there is supporting evidence from mammalian studies that E 2 increases renal expression of PTHrP mRNA without modifying PTH/PTHrP receptor level of expression (Cros et al 1998), thus preventing the renal leak of calcium in osteoporotic women (Nordin et al 1991, Cros et al 1998.…”

Section: Discussionmentioning

confidence: 99%

Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Guerreiro

Fuentes

Canário³

et al. 2002

Journal of Endocrinology

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In all teleost fishes vitellogenesis is triggered and maintained by oestradiol-17 (E 2 ) and is accompanied by an increase of blood plasma calcium and phosphate. The action of this hormone on calcium metabolism was investigated by treating fast-growing immature juvenile sea bream (Sparus aurata) with coconut butter implants alone (control) or implants containing 10 µg/g E 2 . Treatment with E 2 induced the production of circulating vitellogenin, a 2·5-fold increase in plasma ionic Ca 2+ and a 10-fold increase in plasma total calcium, largely bound to protein.In contrast to freshwater species, which obtain most of their calcium from the environment directly through the gills, the intestinal component of calcium uptake of the salt water-living sea bream represented up to 60-70% of the total uptake. The whole body calcium uptake, expressed as the sum of calcium obtained via intestinal and extra-intestinal (likely branchial) routes increased significantly in response to E 2 . Combined influx and unchanged efflux rates resulted in a significant 31% increase in net calcium uptake. There was no evidence for an effect of E 2 on the calcium and phosphate content of the scales or the tartrate-resistant acid phosphatase activity (an index for bone/scale osteoclast activity). While most freshwater fish appear to rely on internal stores of calcium, i.e. bone and/or scales to increase calcium availability, the marine sea bream accommodates calcium-transporting mechanisms to obtain calcium from the environment and preserve internal stores. These observations suggest that a fundamental difference may exist in the E 2 -dependent calcium regulation between freshwater and marine teleosts.

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Section: Discussionmentioning

confidence: 99%

Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Guerreiro

Fuentes

Canário³

et al. 2002

Journal of Endocrinology

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“…Indeed, only very recently the genes for parathyroid hormone (PTH), which is the dom inant hypercalcemic factor for terrestrial vertebrates, were found in fish (Danks et al, 1993). However, earlier, fish were shown to express genes for parathyroid hor mone related protein (PTHrP; Power et al, 2000;Flana gan et al, 2000;Canario et al, 2006). PTHrP behaves in fish as a hypercalcemic hormone and appears key in fish calcium physiology (Ingleton et al, 2002;Trivett et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Abbink

Kulczykowska

Kalamarz

et al. 2008

General and Comparative Endocrinology

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Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5& and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Inter actions/relations between melatonin and calcium balance and the hypercalcemic endocrines PTHrP and calcitriol were assessed. Restrict ing calcium availability in both water and diet had no effect on plasma melatonin, but when calcium was low in the water or absent from food, increased and decreased plasma melatonin was observed, respectively. Fish on a vitamin D-deficient diet (D-fish) showed decreased plasma calcitriol levels and remained normocalcemic. Decreased brain melatonin was found at all sampling times (10-22 weeks) in the D -fish compared to the controls. A positive correlation between plasma Ca2+ and plasma melatonin was found (R2 = 0.19; N = 41; P < 0.01) and brain melatonin was negatively correlated with plasma PTHrP (R2 = 0.78; N = 4; P < 0.05). The posi tive correlation between plasma levels of melatonin and Ca2+ provides evidence that melatonin synthesis is influenced by plasma Ca2+. The decreased melatonin production in the D -fish points to direct or indirect involvement of calcitriol in melatonin synthesis by the pineal organ in teleosts. The hypercalcemic factors PTHrP and calcitriol appeared to be negatively correlated with melatonin and this substantiates an involvement of melatonin in modulating the endocrine response to cope with hypocalcemia. It further points to the importance of Ca2+ in melatonin physiology.

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“…Sections were cut at 4 mm and placed on positively charged slides. Polyclonal antisera were raised against synthetic peptides of elephant shark Pth1(1-14), Pth2 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), and Pthrp (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) by Auspep (West Melbourne, Australia). Sections were stained using the Leica Bond-Max Autostainer (Leica Biosystems, Mount Waverley, Victoria, Australia).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…These include fugu, (5) and gilthead sea bream (Sparus aurata). (6) A number of immunohistochemical studies with antisera to human PTHrP(1-34) had detected the ORIGINAL ARTICLE…”

Section: Introductionmentioning

confidence: 99%

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

Liu¹,

Ibrahim²,

Tay³

et al. 2010

Journal of Bone and Mineral Research

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The development of bone was a major step in the evolution of vertebrates. A bony skeleton provided structural support and a calcium reservoir essential for the movement from an aquatic to a terrestrial environment. Cartilaginous fishes are the oldest living group of jawed vertebrates. In this study we have identified three members of the parathyroid hormone (Pth) gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii). The three genes include two Pth genes, designated as Pth1 and Pth2, and a Pthrp gene. Phylogenetic analysis suggested that elephant shark Pth2 is an ancient gene whose orthologue is lost in bony vertebrates. The Pth1 and Pth2 genes have the same structure as the Pth gene in bony vertebrates, whereas the structure of the Pthrp gene is more complex in tetrapods compared with elephant shark. The three elephant shark genes showed distinct patterns of expression, with Pth2 being expressed only in the brain and spleen. This contrasts with localization of the corresponding proteins, which showed considerable overlap in their distribution. There were conserved sites of localization for Pthrp between elephant shark and mammals, including tissues such as kidney, skin, skeletal and cardiac muscle, pancreas, and cartilage. The elephant shark Pth1(1-34) and Pthrp(1-34) peptides were able to stimulate cAMP accumulation in mammalian UMR106.01 cells. However, Pth2(1-34) peptide did not show such PTH-like biologic activity. The presence of Pth and Pthrp genes in the elephant shark indicates that these genes played fundamental roles before their recruitment to bone development in bony jawed vertebrates. ß

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Genomic structure and expression of parathyroid hormone-related protein gene (PTHrP) in a teleost, Fugu rubripes

Cited by 52 publications

References 33 publications

Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

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