Genomic Structure of a Novel LIM Domain Gene (ZNF185) in Xq28 and Comparisons with the Orthologous Murine Transcript

Heiss, Nina S.; Gloeckner, Gernot; Bächner, Dietmar; Kioschis, Petra; Klauck, Sabine M.; Hinzmann, Bernd; Rosenthal, André; Herman, Gail E.; Poustka, Annemarie

doi:10.1006/geno.1997.4810

Cited by 26 publications

(29 citation statements)

References 48 publications

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“…The presence of ZNF185 mRNA isoforms was further demonstrated by semiquantitative RT-PCR analysis on a panel of 18 normal human tissues (Supplementary Figure 1a). We observed the highest expression of various ZNF185 mRNA isoforms in normal prostate as previously shown by Northern blot (Heiss et al, 1997). In agreement with the partial methylation status of ZNF185 (Vanaja et al, 2003) and the presence of additional mRNA isoforms, we have been able to detect the expression of different ZNF185 protein isorfoms in the several PCa cell lines using ZNF185-specific antibody (Supplementary Figure 1b).…”

Section: Discussionsupporting

confidence: 88%

“…Importantly, our cDNA extended the 5 0 -end of the ZNF185 protein coding sequence corresponding to the actin-binding region or ATD. The ATD is necessary for both targeting of ZNF185 to actincytoskeleton and its growth regulatory activity, whereas the previously reported ZNF185 452 without the ATD possibly represents a protein isoform not associated with actin-cytoskeleton (Heiss et al, 1997). The presence of ZNF185 mRNA isoforms was further demonstrated by semiquantitative RT-PCR analysis on a panel of 18 normal human tissues (Supplementary Figure 1a).…”

Section: Discussionmentioning

confidence: 77%

“…The entire 3 0 -untranslated region (3 0 -UTR) is derived from exon 23, the last and the largest exon of this gene. Importantly, this cDNA contains additional exons in the 5 0 -end, which encodes an extended 144 a.a. residues in the NH 2 -terminus of the protein compared to the previously reported ZNF185 (Heiss et al, 1997).…”

Section: Resultsmentioning

confidence: 90%

“…ZNF185 was originally isolated in an effort to identify transcripts from the DXS52 region on chromosome Xq28 using a positional cloning approach (Heiss et al, 1997). This region is also reported to harbor a gene involved in PCa susceptibility (Xu et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…ZNF185 mRNA expression was detected in several normal human tissues by Northern blot analysis, and was most abundant in the prostate. A ZNF185 cDNA encoding 452 amino-acid (a.a.) residues with a single C-terminallocated LIM domain was assembled based on the sequence of an expressed sequence tagged (EST) clone and a genomic DNA sequence (Heiss et al, 1997). The LIM domain is a cysteine-and histidine-rich double zinc-finger motif named after the three-homeodomain proteins: Lin-l 1, Isl-1 and Mec-3 (Way and Chalfie, 1988;Freyd et al, 1990;Karlsson et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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ZNF185, an actin–cytoskeleton-associated growth inhibitory LIM protein in prostate cancer

2006

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We have recently identified ZNF185 as a gene that is downregulated in prostate cancer (PCa), in part via epigenetic alteration, and maybe associated with disease progression. In this study, we cloned the ZNF185 cDNA from normal human prostate tissues and investigated its biological function. We show that ZNF185 is a novel actin-cytoskeleton-associated Lin-l 1, Isl-1 and Mec-3 (LIM) domain-containing protein that localizes to F-actin structures, and is enriched at focal adhesions. We find that the NH 2 -terminal region, which we designate the actintargeting domain, facilitates ZNF185 binding to actin in vitro and is both necessary and sufficient to mediate actin-cytoskeleton targeting of ZNF185, whereas the LIM domain, which is localized in the COOH-terminus is dispensable for this phenomenon. Interestingly, ectopic expression of full-length ZNF185, but not a mutant lacking the actin-targeting domain, could suppress proliferation and anchorage-independent growth of PCa cells. Together, our data suggest that ZNF185 may function as a tumor-suppressor protein by associating with the actincytoskeleton.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ZNF185, an actin–cytoskeleton-associated growth inhibitory LIM protein in prostate cancer

2006

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ZNF185‐derived peptide induces fertility suppression in mice

Fan

Zhao

Pan

et al. 2018

Journal of Peptide Science

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Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in male reproduction. However, it is unclear whether ZNF185 may be a target candidate for contraceptive vaccines. In this study, antigenic peptides derived from ZNF185 were prepared, and their immune contraceptive effects were investigated using mice. Results from enzyme‐linked immunosorbent assay (ELISAs) showed that peptide immunization induced an antibody titre increase that reached a peak in week 12. Peptide‐3 and peptide‐4 were then chosen for subsequent experiments. The results of the fertility assays showed that peptide immunization inhibited the mating and fertility rates of the mice, whereas there were no obvious changes in the number of pups per litter. Subsequently, epididymal sperm was analysed. The results demonstrated that the sperm count and sperm motility were significantly decreased in the peptide group, while the amount of abnormal sperm was significantly increased in the peptide‐3 group. The male reproductive organs were also evaluated. There were no obvious differences in testis or epididymal weights, in the diameters of the seminiferous tubules, or in the thicknesses of the seminiferous epithelium between the peptide group and the phosphate buffer saline (PBS) group. In addition, histological analysis indicated that there were no obvious pathologic changes in testis and epididymal histology in the peptide group; however, the number of spermatozoa present in the epididymal lumen of the peptide group was significantly decreased when compared with the PBS group. Our study demonstrates for the first time that peptides derived from ZNF185 may induce fertility suppression in mice without damaging reproductive organs. These peptides have the potential to be used as a male contraceptive vaccine.

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Analysis of a Human cDNA Containing a Tissue-Specific Alternatively Spliced LIM Domain

Putilina

Jaworski

Gentleman

et al. 1998

Biochemical and Biophysical Research Communications

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Genomic Structure of a Novel LIM Domain Gene (ZNF185) in Xq28 and Comparisons with the Orthologous Murine Transcript

Cited by 26 publications

References 48 publications

ZNF185, an actin–cytoskeleton-associated growth inhibitory LIM protein in prostate cancer

ZNF185, an actin–cytoskeleton-associated growth inhibitory LIM protein in prostate cancer

ZNF185‐derived peptide induces fertility suppression in mice

Analysis of a Human cDNA Containing a Tissue-Specific Alternatively Spliced LIM Domain

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