Genomics of Helper Component Proteinase Reveals Effective Strategy for Papaya Ringspot Virus Resistance

Mangrauthia, Satendra K.; Singh, Priyanka; Praveen, Shelly

doi:10.1007/s12033-009-9205-5

Cited by 18 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One gram of leaf tissues from PRSV-infected plants was ground in 0.1 M potassium phosphate buffer, pH 7.0. Plants were dusted with Carborundum and PRSV-infected leaf extract was inoculated on the youngest fully-expanded leaves of each plant as described by Mangrauthia and coworkers [30].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of the Host Proteasome Facilitates Papaya Ringspot Virus Accumulation and Proteosomal Catalytic Activity Is Modulated by Viral Factor HcPro

et al. 2012

Self Cite

View full text Add to dashboard Cite

The ubiquitin/26S proteasome system plays an essential role not only in maintaining protein turnover, but also in regulating many other plant responses, including plant–pathogen interactions. Previous studies highlighted different roles of the 20S proteasome in plant defense during virus infection, either indirectly through viral suppressor-mediated degradation of Argonaute proteins, affecting the RNA interference pathway, or directly through modulation of the proteolytic and RNase activity of the 20S proteasome, a component of the 20S proteasome, by viral proteins, affecting the levels of viral proteins and RNAs. Here we show that MG132, a cell permeable proteasomal inhibitor, caused an increase in papaya ringspot virus (PRSV) accumulation in its natural host papaya (Carica papaya). We also show that the PRSV HcPro interacts with the papaya homologue of the Arabidopsis PAA (α1 subunit of the 20S proteasome), but not with the papaya homologue of Arabidopsis PAE (α5 subunit of the 20S proteasome), associated with the RNase activity, although the two 20S proteasome subunits interacted with each other. Mutated forms of PRSV HcPro showed that the conserved KITC54 motif in the N-terminal domain of HcPro was necessary for its binding to PAA. Co-agroinfiltration assays demonstrated that HcPro expression mimicked the action of MG132, and facilitated the accumulation of bothtotal ubiquitinated proteins and viral/non-viral exogenous RNA in Nicotiana benthamiana leaves. These effects were not observed by using an HcPro mutant (KITS54), which impaired the HcPro – PAA interaction. Thus, the PRSV HcPro interacts with a proteasomal subunit, inhibiting the action of the 20S proteasome, suggesting that HcPro might be crucial for modulating its catalytic activities in support of virus accumulation.

show abstract

Section: Methodsmentioning

confidence: 99%

“…PRSV infection was detected and quantified serologically using the plate trapped-antigen enzyme-linked immunosorbant assays (ELISA) method [30] in Maxisorb microtiter plates (Nunc, Roskilde, Denmark), using 0.1 g fresh leaf tissue. All incubations were performed at room temperature, unless stated otherwise.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the Host Proteasome Facilitates Papaya Ringspot Virus Accumulation and Proteosomal Catalytic Activity Is Modulated by Viral Factor HcPro

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Management Of Plant Pathogenic Virusesmentioning

confidence: 99%

Biotechnology and Plant Disease Control-Role of RNA Interference

Wani¹,

Sanghera²,

Singh³

2010

AJPS

View full text Add to dashboard Cite

show abstract

“…In planta expression of PRSV-HcPro affects developmental biology of plants, suggesting the interference of suppressor protein in micro RNA-directed regulatory pathways of plants. Besides facilitating the establishment of PRSV, it showed strong positive synergism with other heterologous viruses as well (Mangrauthia et al, 2010). Therefore, resistance in transgenic papaya can be overcome by PRSV with distant homology to the transgene, or by PRSV strains with HCPro that can sufficiently suppress the silencing mechanism of transgenic papaya.…”

Section: Transgenic Papaya In Taiwanmentioning

confidence: 99%

Genetic Engineering for Viral Disease Management in Plants

Wani

Sanghera

2010

Not Sci Biol

View full text Add to dashboard Cite

Viral diseases are a major threat to world agriculture and breeding resistant varieties against these viruses is one of the major challenge faced by plant virologists and biotechnologists. The development of the concept of pathogen derived resistance gave rise to strategies ranging from coat protein based interference of virus propagation to RNA mediated virus gene silencing. Much progress has been achieved in protecting plants against these RNA and DNA viruses. In this review, the most recent transgene based approaches for viral disease management in plants will be discussed.

show abstract

Genomics of Helper Component Proteinase Reveals Effective Strategy for Papaya Ringspot Virus Resistance

Cited by 18 publications

References 27 publications

Inhibition of the Host Proteasome Facilitates Papaya Ringspot Virus Accumulation and Proteosomal Catalytic Activity Is Modulated by Viral Factor HcPro

Inhibition of the Host Proteasome Facilitates Papaya Ringspot Virus Accumulation and Proteosomal Catalytic Activity Is Modulated by Viral Factor HcPro

Biotechnology and Plant Disease Control-Role of RNA Interference

Genetic Engineering for Viral Disease Management in Plants

Contact Info

Product

Resources

About