Genomics of
            <i>Agrobacterium</i>
            –Plant Interaction: An Approach to Refine the Plant Transformation Technology

Anand, Ajith; Vaghchhipawala, Zarir; Mysore, Kirankumar S.

doi:10.1002/9780470958988.ch2

Cited by 4 publications

(2 citation statements)

References 87 publications

(146 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The refinements consist of improving treatment conditions to promote Agrobacterium-mediated transformation, e.g., vortex-mediated transformation of cold-treated seedlings (Rosellini & Veronesi, 2007;Weeks, Ye & Rommens, 2008), using A. tumefaciens strains that exhibit extremely high transformation efficiency (De Vetten et al 2003). Most of these refinements take advantage of recent advances in identifying plant proteins and other factors within the transformation process and characterizing the molecular mechanism for successful T-DNA incorporation Anand, Vaghchhipawala & Mysore (2010); Barampuram & Zhang (2011).…”

Section: Marker-free Strategies or Systemsmentioning

confidence: 99%

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

Dormatey

Sun

Ali

et al. 2021

PeerJ

View full text Add to dashboard Cite

Antibiotic and herbicide resistance genes are the most common marker genes for plant transformation to improve crop yield and food quality. However, there is public concern about the use of resistance marker genes in food crops due to the risk of potential gene flow from transgenic plants to compatible weedy relatives, leading to the possible development of “superweeds” and antibiotic resistance. Several selectable marker genes such as aph, nptII, aaC3, aadA, pat, bar, epsp and gat, which have been synthesized to generate transgenic plants by genetic transformation, have shown some limitations. These marker genes, which confer antibiotic or herbicide resistance and are introduced into crops along with economically valuable genes, have three main problems: selective agents have negative effects on plant cell proliferation and differentiation, uncertainty about the environmental effects of many selectable marker genes, and difficulty in performing recurrent transformations with the same selectable marker to pyramid desired genes. Recently, a simple, novel, and affordable method was presented for plant cells to convert non-metabolizable phosphite (Phi) to an important phosphate (Pi) for developing cells by gene expression encoding a phosphite oxidoreductase (PTXD) enzyme. The ptxD gene, in combination with a selection medium containing Phi as the sole phosphorus (P) source, can serve as an effective and efficient system for selecting transformed cells. The selection system adds nutrients to transgenic plants without potential risks to the environment. The ptxD/Phi system has been shown to be a promising transgenic selection system with several advantages in cost and safety compared to other antibiotic-based selection systems. In this review, we have summarized the development of selection markers for genetic transformation and the potential use of the ptxD/Phi scheme as an alternative selection marker system to minimize the future use of antibiotic and herbicide marker genes.

show abstract

Section: Marker-free Strategies or Systemsmentioning

confidence: 99%

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

Dormatey

Sun

Ali

et al. 2021

PeerJ

View full text Add to dashboard Cite

show abstract

“…Such refinements include optimizing treatment conditions to promote Agrobacterium-mediated transformation, e.g., via vortex-mediated transformation of cold-treated seedlings Weeks et al, 2008), using A. tumefaciens strains exhibiting extremely high transformation efficiency (de Vetten et al, 2003), or improving plant tissue culture. Some of these refinements exploit the progress made in recent years in identifying plant proteins and other factors involved in the transformation process and characterizing the precise molecular mechanism leading to successful integration of the T-DNA (Anand et al, 2010;Barampuram and Zhang, 2011).…”

Section: F Marker-less Transformationmentioning

confidence: 99%

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Breyer

Kopertekh

Reheul

2014

Critical Reviews in Plant Sciences

View full text Add to dashboard Cite

Effect of Agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar

TeRonde

Meyer

et al. 2015

Plant Cell Rep

View full text Add to dashboard Cite

Improving Agrobacterium -mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. Agrobacterium-mediated maize transformation is a well-established method for gene testing and for introducing useful traits in a commercial biotech product pipeline. To develop a highly efficient maize transformation system, we investigated the effect of two Agrobacterium tumefaciens strains and three different binary plasmid origins of replication (ORI) on transformation frequency, vector backbone insertion, single copy event frequency (percentage of events which are single copy for all transgenes), quality event frequency (percentage of single copy events with no vector backbone insertions among all events generated; QE) and usable event quality frequency (transformation frequency times QE frequency; UE) in an elite maize cultivar PHR03. Agrobacterium strain AGL0 gave a higher transformation frequency, but a reduced QE frequency than LBA4404 due to a higher number of vector backbone insertions. Higher binary plasmid copy number positively correlated with transformation frequency and usable event recovery. The above findings can be exploited to develop high-throughput transformation protocols, improve the quality of transgenic events in maize and other plants.

show abstract

Genomics of Agrobacterium –Plant Interaction: An Approach to Refine the Plant Transformation Technology

Cited by 4 publications

References 87 publications

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

Alternatives to Antibiotic Resistance Marker Genes forIn VitroSelection of Genetically Modified Plants – Scientific Developments, Current Use, Operational Access and Biosafety Considerations

Effect of Agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar

Contact Info

Product

Resources

About