2012
DOI: 10.1590/s0102-05362012000300009
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Genotipagem de polimorfismos associados com sistemas de macho-esterilidade em acessos de cebola adaptados ao Brasil

Abstract: A produção em escala comercial de sementes híbridas de cebola (Allium cepa) tem sido conduzida com o emprego de dois sistemas de macho-esterilidade do tipo genética-citoplasmática (CMS-S e CMS-T) em associação ao citoplasma normal (macho-fértil). No entanto, a análise molecular desses diferentes tipos citoplasmáticos ainda não está disponível para um grande número de acessos de cebola adaptados para cultivo em regiões tropicais. Além de adaptação às condições edafoclimáticas do Brasil, muitos desses acessos ap… Show more

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Cited by 4 publications
(5 citation statements)
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“…Marker-assisted selection in onions has been used to differentiate 'N', 'S', and 'T' cytoplasm (Khar and Saini, 2016;Patil et al, 2016;Ragassi et al, 2012Ragassi et al, , 2010Santos et al, 2010), contributing to reduce the number of test crosses required to identify 'A' and 'B' lines. Markers for the Ms locus, developed by Huo et al (2015) and Kim et al (2015), enabled the full assisted identification of 'A' and 'B' lines, and have been applied in Indian germplasm (Khar and Saini, 2016) and in Brazilian germplasm (Ferreira et al, 2017) for the simultaneous identification of the cytoplasm type and recessive condition of the Ms allele.…”
Section: Discussionmentioning
confidence: 99%
“…Marker-assisted selection in onions has been used to differentiate 'N', 'S', and 'T' cytoplasm (Khar and Saini, 2016;Patil et al, 2016;Ragassi et al, 2012Ragassi et al, , 2010Santos et al, 2010), contributing to reduce the number of test crosses required to identify 'A' and 'B' lines. Markers for the Ms locus, developed by Huo et al (2015) and Kim et al (2015), enabled the full assisted identification of 'A' and 'B' lines, and have been applied in Indian germplasm (Khar and Saini, 2016) and in Brazilian germplasm (Ferreira et al, 2017) for the simultaneous identification of the cytoplasm type and recessive condition of the Ms allele.…”
Section: Discussionmentioning
confidence: 99%
“…These PCR markers have been used to predict the frequency of the cytoplasm and Ms locus in onion populations from various countries. The frequency of the T cytoplasm (48%) was more than the S cytoplasm (15%) in Korean onion [217], the 2% male-sterile line (both the S and T cytoplasms) [241], the S (56%) and T (25.8%) cytoplasms in Brazilian onion [242], and the S cytoplasm (2.15%) in Indian onion VL Piaz-3 [243], whereas the S cytoplasm in the range of 88% (Sel. 121-2), 33% (Pusa Red), and 17% (Pusa Madhavi) was reported by Khar and Saini [235].…”
Section: Male Sterility and Hybrid Developmentmentioning
confidence: 96%
“…121-2), 33% (Pusa Red), and 17% (Pusa Madhavi) was reported by Khar and Saini [235]. Until 2015, the primers reported by Sato [215] and Engelke et al [216] were mostly used for cytoplasm determination [242][243][244]. Recently, new primers for the cytoplasm [217,218] and Ms locus [219,234] have been utilized for the identification of the cytoplasm and Ms locus in onions from Brazil [245], India [238,246], and South Africa [247].…”
Section: Male Sterility and Hybrid Developmentmentioning
confidence: 99%
“…The use of hybrid seeds by onion producers has increassed because the yield and quality of bulbs are greater than those of open-pollinated cultivars (May et al, 2007;Ragassi et al, 2012). This perspective encourages breeding programs to identify superior lineages that can be used to develop new hybrids, aiming to meet demands in different growing environments (Faria et al, 2012;Quartieiro et al, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…Few studies have estimated the genetic divergence of onion genotypes to promote productive breeding (Buzar et al, 2007;Ragassi et al, 2012;Wamser et al, 2012). We evaluated the correlation between genetic dissimilarity of inbred onion lines and the SCAs determined by diallelic crosses.…”
Section: Introductionmentioning
confidence: 99%