Genotipificación de aislamientos clínicos del complejo Cryptococcus neoformans/Cryptococcus gattii obtenidos en el Hospital «Dr. Julio C. Perrando», de la ciudad de Resistencia (Chaco, Argentina)

Abstract

Cryptococcosis is a fungal infection caused by yeast species of Cryptococcus genus, particularly Cryptococcus neoformans/Cryptococcus gattii species complex. The knowledge of the cryptococcosis casuistic in northeastern Argentina is scarce and there is no information about the molecular types circulating in this area. The aim of this study was to genotyping C. neoformans/C. gattii complex clinical isolates obtained at Hospital "Dr. Julio C. Perrando", Resistencia city (Chaco, Argentina), in order to determine species, variety and molecular type. During two years and one month 26 clinical isolates were studied. Using conventional and molecular methods one isolate was identified as C. gattii VGI type, and 25 isolates as C. neoformans var. grubii; 23 of these belonged to VNI type and two belonged to VNII type. This data is a contribution to the knowledge of cryptococcosis epidemiology in Argentina and the first report about C. neoformans/ C. gattii complex molecular types from clinical isolates in northeastern Argentina.

Molecular typing, in vitro susceptibility and virulence of Cryptococcus neoformans/Cryptococcus gattii species complex clinical isolates from south‐eastern Brazil

“…Among the clinical isolates, we identified 84.7% C. neoformans molecular type VNI, 4.2% C. neoformans molecular type VNII and 11.1% C. deuterogattii molecular type VGII. In Latin America, molecular types VNI and VGII have a high prevalence 5,8,31 . In Brazil, C. neoformans molecular type VNI is prevalent in the south‐eastern and southern States.…”

Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors

“…The remaining four (3.82%) VNII isolates were recovered from CSF (L66, LN3, LN4, and LN12). This molecular type has been described from different countries among clinical and environmental isolates and was identified by molecular techniques with frequencies ranging from 0.2% to 7.7% [ 46 , 49 , 52 , 57 ].…”

The status of cryptococcosis in Latin America

“… Molecular data have been combined from studies reported in Argentina ( Meyer et al. 2003 , Refojo et al 2009 , Cattana et al 2013 , Mazza et al 2013 , Cattana et al 2014 , Cattana et al 2015 , Cicora et al 2015 , Arechavala et al 2017 ), Brazil ( Casali et al 2003 , Trilles et al 2003 , Igreja et al 2004 , Abegg et al 2006 , Ribeiro et al 2006 , Matsumoto et al 2007 , Lugarini et al 2008 , Ribeiro & Ngamskulrungroj 2008 , dos Santos et al 2008 , Trilles et al 2008 , Costa et al 2009 , Andrade-Silva et al 2010 , Pinto Jr et al 2010 , Souza et al 2010 , Ferreira-Paim et al 2011, d , da Silva et al 2012 , Freire et al 2012 , Silva et al 2012 , Cardoso et al 2013 , Tsujisaki et al 2013 , Anzai et al 2014 , Nascimento et al 2014 , Brito-Santos et al 2015 , Headley et al 2015 , Castro e Silva et al 2016 , Figueiredo et al 2016 , Lomes et al 2016 , Souto et al 2016 , Aguiar et al 2017, d , da Silva et al 2017) , Chile ( Calvo et al 2001 , Meyer et al. 2003 , Solar et al 2015) , Colombia ( Meyer et al 2003 , Escandón et al 2006 , Escandón et al 2010 , Firacative et al 2011 , Lizarazo et al 2012 , Lizarazo et al 2014a , Lizarazo et al 2014b , Noguera et al 2015 , Cogliati et al ...…”