Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays

Leite-Silva, Cleide; Gusmão, Cássia Lima Silva; Takahashi, Catarina Satie

doi:10.1590/s1415-47572007000100019

Cited by 23 publications

(4 citation statements)

References 30 publications

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“…The existence of SA’s various antioxidant scavengers, such as fucoidans, may explain its protective activity against Cr-induced genotoxicity [ 67 ]. The antigenotoxicity of fucoidan was previously identified against doxorubicin-induced DNA damage and proved in terms of chromosome aberrations and comet in human lymphocytes [ 68 ]. Accordingly, other study reported that fucoidan protects mice from 4-NQO-induced genetic damage in terms of LPO, 8-OHdG, micronuclei, comet and apoptosis [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

2022

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Oral exposure to chromium hexavalent [Cr(VI)] has disastrous impacts and affects many people worldwide. Cr(VI) triggers neurotoxicity via its high oxidation potential by generating high amount of ROS. Meanwhile, alginates are known by their chelating activity and ability to bind heavy metals and toxins, in addition to their antioxidant, anti-inflammatory, and anti-apoptotic activities. So, this study aimed to explore the neuroprotective potential of sodium alginate (SA) against cellular injury, DNA damage, macromolecule alterations, and apoptosis induced by oral ingestion of Cr. Forty Wistar male rats were divided into 4 groups; group I: standard control ingested with the vehicle solution, group II: Cr-intoxicated group received 10 mg/kg b.w. of potassium dichromate orally by gavage and kept without treatment, group III: SA group in which rats were orally exposed to 200 mg/kg b.w. of SA only, and group IV: SA-treated group that received 200 mg/kg b.w. of SA along with Cr for 28 consecutive days. Neurotransmitters such as Acetyl choline esterase (AchE), Monoamine oxidase A (MAOA) concentrations, Dopamine (DA) and 5-Hydroxytryptamine (5-HT) levels were assessed in brain homogenate tissues. Neurobiochemical markers; NAD+ and S100B protein were investigated in the brain tissues and serum, respectively. Levels of HSP70, caspase-3, protein profiling were evaluated. DNA damage was determined using the Comet assay. Results revealed a significant reduction in the AchE and MAOA concentrations, DA, 5-HT, and NAD+ levels, with an increase in the S100B protein levels. Cr(VI) altered protein pattern and caused DNA damage. High levels of HSP70 and caspase-3 proteins were observed. Fortunately, oral administration of SA prevented the accumulation of Cr in brain homogenates and significantly improved all investigated parameters. SA attenuated the ROS production and relieved the oxidative stress by its active constituents. SA can protect against cellular and DNA damage and limit apoptosis. SA could be a promising neuroprotective agent against Cr(VI)-inducing toxicity.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

2022

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“…Treatment of human lymphocyte cultures with ADR induced chromosome aberrations and DNA damage. 29 Khan et al 30 reported that ADR and idarubicin caused chromosomal aberration and micronucleus in cultured human lymphocytes. Dimanche-Boitrel et al 31 mentioned that most of the anticancer therapies induce tumor cell death through the induction of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Aqueous Fenugreek Seed Extract Ameliorates Adriamycin-Induced Cytotoxicity and Testicular Alterations in Albino Rats

2012

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The present work studied the effect of fenugreek seed extracts on cytotoxicity and testicular damage induced by adriamycin (ADR) in albino rats. Administrating animals with ADR caused significant increase in the percentage of chromosomal aberrations, decreased the mitotic index, and induced DNA damage in bone marrow. Testes of ADR-treated rats showed many histopathological alterations and the number of sperm head abnormalities increased. Moreover, the concentration of malondialdehyde (MDA) increased and the activity of catalase (CAT) and superoxide dismutase (SOD) decreased in the testis. Treating animals with ADR and aqueous seed extracts of fenugreek led to an improvement in the cytogenetic effect and testicular alterations induced by ADR. Lipid peroxidation was reduced and the activities of CAT and SOD were increased. In conclusion, the results indicated that fenugreek seeds ameliorated the cytotoxicity and testicular alterations induced by ADR in albino rats and this may be mediated by its potent antioxidant effects.

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“…The degree of DNA damage can be represented using parameters like tail moment, olive moment, and percentage of DNA in tail. The brown seaweed Fucus vesiculosus was evaluated for genotoxic activity by Leite-Silva et al [ 34 ] and the results suggest that the seaweed did not exhibit genotoxic effect in cultured human lymphocytes. Similarly the non-genotoxic property of G. acerosa and S. wightii was evaluated and the results show that the extracts did not induce DNA damage, which was verified by the decrease in the parameters like tail moment, olive moment, and percentage of DNA in tail.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Mutagenic Effect ofG. acerosaandS. wightiiinS. typhimurium(TA 98, TA 100, and TA 1538 strains) and Evaluation of Their Cytotoxic and Genotoxic Effect in Human Mononuclear Cells: A Non-Clinical Study

Syad

Kasi

2014

BioMed Research International

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The marine red algae (Gelidiella acerosa and Sargassum wightii) possessing excellent antioxidant and anticholinesterase activity were subjected to toxicity evaluation for a deeper understanding of other bioprotective properties of seaweeds. Cytotoxic evaluation was done by trypan blue exclusion, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays using human PBMC (peripheral blood mononuclear cells) and RBC (red blood cells) lysis assay using human erythrocytes. Mutagenicity of the seaweeds was analyzed by Ames salmonella mutagenicity test with the histidine dependent mutant strains TA 98, TA100 and TA 1538. Genotoxic activity was verified in PBMC by comet assay. The results suggest that benzene extract of G. acerosa (BEGA) and dichloromethane extract of S. wightii (DMESW) did not show cytotoxic effect both in PBMC and erythrocytes. Evaluation of mutagenic activity suggests that the seaweeds did not cause any mutagenic effects both in the absence and the presence of S9 microsomal fraction in all the three Salmonella mutant strains. Results of genotoxic study showed that PBMC treated with seaweed extracts (1 mg/mL) exhibit less or no damage to cells, thus proving the non-genotoxic effect of the extract. Since these in vitro non-clinical studies clearly demonstrate the non-toxic nature of the seaweeds, they could be exploited for further characterization, which would result in development of novel and safe therapeutic entities.

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Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays

Cited by 23 publications

References 30 publications

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

Aqueous Fenugreek Seed Extract Ameliorates Adriamycin-Induced Cytotoxicity and Testicular Alterations in Albino Rats

Assessment of Mutagenic Effect ofG. acerosaandS. wightiiinS. typhimurium(TA 98, TA 100, and TA 1538 strains) and Evaluation of Their Cytotoxic and Genotoxic Effect in Human Mononuclear Cells: A Non-Clinical Study

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