Genotoxic and clastogenic activity of saponins extracted from Nauclea bark as assessed by the micronucleus and the comet assays in Chinese Hamster Ovary cells

Liu, W.; Giorgio, Carole Di; Lamidi, M.; Élias, Riad; Ollivier, E.; Méo, M.P. De

doi:10.1016/j.jep.2011.05.005

Cited by 29 publications

(16 citation statements)

References 26 publications

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“…The evaluation of micronucleus frequencies in vivo is one of the primary genotoxicity tests recommended internationally by regulatory agencies for product safety assessment [ 21 ]. Based on the international working group that evaluates the micronucleus test, this should be used when no signs of toxicity at maximal possible concentration are seen [ 22 , 23 ]. Accordingly, from the acute toxicity test, the dose of 250 mg/kg was selected for the genotoxicity test [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass)

Padilla-Camberos

Martínez-Velázquez

Flores-Fernández

et al. 2013

The Scientific World Journal

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The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle); therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

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Section: Discussionmentioning

confidence: 99%

Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass)

Padilla-Camberos

Martínez-Velázquez

Flores-Fernández

et al. 2013

The Scientific World Journal

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“…[17] Moreover, it was shown that saponins mixture (saponins A, D, E, G, J, K) obtained from Nauclea species induced synergistic in vitro DNA-damage and chromosome mutations in mammalian cells. [18] Also, the DNA damaging effect of the fractions of Glinus lotoides containing hopane-type saponins was reported. [19] Similarly, in the present study, we found that a significant DNA damaging effect of the nebuloside A at concentrations of 5, 10, 20, 50, μg/mL on human blood DNA using alkaline COMET assay.…”

Section: Resultsmentioning

confidence: 99%

“…Medicinal plants are commonly rich in different phytochemicals which have multiple biological effects. [20 , 21] Liu et al [18] reported that the genotoxicity of the saponins present in Nauclea genus was probably due to their capacity to reduce cell defense against oxidative stress through the inhibition of glutathione-S-transferase activity. Similarly, nebuloside A may reduce the cell defense against oxidative stress and cause the DNA damage.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicological Assessment of Nebuloside-A a Triterpenoid Saponin Compound on Whole Blood DNA

Arslan

İli

2015

International Journal of Food Properties

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We investigated the genotoxic effect of nebuloside-A on whole blood DNA by using alkaline Single Cell Gel Electrophoresis Comet assay. Saponins are a widespread class of bioactive compounds produced by many plant species. Nebuloside A was isolated from baby's-breath (Gypsophila arrostii) by high performance liquid chromatography method. Mass spectra obtained by electrospray ionizationtime of flight-mass spectrometry in a negative ion mode to reduce the protonation. Complete nuclear magnetic resonance spectroscopy assignments of nebuloside A was achieved by using 2D-nuclear magnetic resonance spectroscopy techniques, such as double-quantum filtered correlation spectroscopy, heteronuclear single quanthum correlation, heteronuclear multiple-bond correlation, and total correlation spectroscopy. DNA damage was measured in total arbitrary units by visual scoring of comets in different concentrations of nebuloside A and H 2 O 2 treatments. We found that significant dose dependent relationship between nebuloside A and its genotoxic effect.

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“…The genotoxic and antigenotoxic effects of some saponins (the parent drug) have been described. For instance, Liu et al [32] reported the ability of a mix of saponins extracted from plants of the Nuclea genus, widely used in African traditional medicine, to elicit DNA damage when tested on Chinese Hamster Ovary cells. On the other hand, the antigenotoxic effect of saponins has also been described.…”

Section: Discussionmentioning

confidence: 99%

Effect of Hecogenin on DNA instability

et al. 2016

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Hecogenin is a sapogenin found in Agave species in high quantities and is responsible for the many therapeutic effects of these medicinal plants. In addition, this compound is also widely used in the pharmaceutical industry as a precursor for the synthesis of steroidal hormones and anti-inflammatory drugs. Despite Hecogenin being widely used, little is known about its toxicological properties. Therefore, the present study aimed to investigate the cytotoxic, genotoxic and mutagenic effects of Hecogenin on HepG2 cells. Cytotoxicity was analyzed using the MTT test. Then, genotoxic and mutagenic potentials were assessed by comet assay and cytokinesis-block micronucleus assay, respectively. Cytotoxic effect was observed only when cells were exposed to concentrations of Hecogenin equal or higher than 100 μM. Although a lower concentration of Hecogenin caused DNA damage, a reduction on nuclear mutagenic markers in HepG2 cells was observed. The results indicated that Hecogenin treatment generated DNA damage, but in fact it would be repaired, avoiding dissemination of the damage throughout the cell division. Further studies need to be performed to confirm the observed protective effect of Hecogenin against genomic instability.

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Genotoxic and clastogenic activity of saponins extracted from Nauclea bark as assessed by the micronucleus and the comet assays in Chinese Hamster Ovary cells

Cited by 29 publications

References 26 publications

Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass)

Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass)

Genotoxicological Assessment of Nebuloside-A a Triterpenoid Saponin Compound on Whole Blood DNA

Effect of Hecogenin on DNA instability

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