Genotoxic evaluation of sodium nitroprusside in Aspergillus nidulans

Abstract

The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH + //uvsH + ) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 mM, 80 mM and 160 mM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.

Genotoxic Potentials of Natural Products Detected by a Short-Term Test Using Diploid Strains of Aspergillus nidulans

“…Despite such trends present shortterm test has been used to disclose suspected genotoxicity rapidly and efficiently. Besides, the test is very sensitive as genotoxicity can be detected even after application of low doses of a given agent, such as UV [13], benzene fumes [14], sodium nitroprusside [40], benznidazole [41], cisplatin and cytosine arabinoside [42] and X-ray [43]. These characters altogether allied to test inexpensiveness, suggest involved eukaryote microorganism (A. nidulans) can be proposed as a cell model to guide early approaches towards rapid screening of genotoxic potential and biological activities of many chemicals.…”

Nitric oxide as a developmental and metabolic signal in filamentous fungi

“…In A. nidulans , for instance, an artificial elevation of NO led to reduced conidiation and increased formation of sexual fruiting bodies (Marcos et al, ). The reduction in conidiation may be due to the impairment of transcriptional activation of sporulation‐specific genes induced by high levels of exogenous NO (Chiuchetta & Castro‐Prado, ). Recently, it has also been shown that different NO levels exerted different impacts on the expression of nsdD for regulating sexual development and brlA for regulating conidiation (Marcos et al, ) (Figure ).…”

Effects of abscisic acid and nitric oxide on trap formation and trapping of nematodes by the fungus Drechslerella stenobrocha AS6.1

“…Although there is a little information about the role of NO in microorganisms (Ninnemann & Maier 1996;Chiuchetta & de Castro-Prado 2005;Wang & Higgins 2005), a recent report showed that NO played a key role in appressorium formation by Blumeria graminis f.sp. hordei (Prats et al 2008).…”

“…However, evidence for the presence and role of NO in microorganisms is limited. Studies with the fungi Neurospora crassa, Colletotrichum coccodes, and Aspergillus nidulans suggest that NO may have a regulatory effect in conidiation and germination (Ninnemann & Maier 1996;Chiuchetta & de Castro-Prado 2005;Wang & Higgins 2005). Confocal microscopy indicated that NO influences appressorium formation in the plant-pathogenic fungus Blumeria graminis f.sp.…”