As parasitoses intestinais ainda constituem um sério problema de saúde pública no Brasil, apresentandomaior prevalência em populações de nível socioeconômico mais baixo e com condições precárias de saneamentobásico. O objetivo desse trabalho foi avaliar a ocorrência de parasitoses intestinais na populaçãodo município de Goioerê, PR, por meio de exames parasitológicos de fezes, e desenvolver métodos decontrole das enteroparasitoses. Foram analisadas 195 amostras fecais, das quais 38% estavam parasitadas,89% das amostras positivas apresentaram monoparasitismo e 11% biparasitismo. Os parasitas mais prevalentesforam Ascaris lumbricoides (39,2%) seguido por Entamoeba coli (31,6%) e Giardia lamblia (13,5%).Após levantamento de dados, medidas preventivas a partir de palestras educativas foram realizadas a fi mde minimizar as contaminações enteroparasitárias e seus efeitos nocivos. As palestras educativas foramrealizadas em dois momentos, um para crianças e outro para jovens e adultos. Essa atividade abordoutemas como: modo de contaminação e prevenção de parasitoses, efeitos dos parasitas no organismohumano e alerta sobre o risco da automedicação voltada ao tratamento das enteroparasitoses.

The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH + //uvsH + ) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 mM, 80 mM and 160 mM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.

Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10 -3 and 5.0 x 10 -3 µM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.

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Doxorubicin and etoposide are intercalating agents that inhibit the action of the enzyme topoisomerase II. Both drugs present therapeutic activity in numerous human neoplasms In the present work the recombinagenic potential of these drugs was evaluated by ascomycete Aspergillus nidulans. Their effects on the asexual cycle of A. nidulans was also appraised. Two heterozygous diploid strains of A. nidulans, a wild (uvsH+// uvsH+) and a defective to the DNA repair (uvsH//uvsH) were used. The drugs' recombinagenic potential was evaluated by their capacity to induce homozygosis of recessive genes from heterozygous cells. Both drugs have a recombinagenic effect on diploid cells of A. nidulans. Doxorubicin and etoposide are potentially capable to induce secondary malignancies, mediated by the mitotic crossing-over in eukaryotic cells.