Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM2.5) in human lung epithelial A549 cells

Billet, Sylvain; Abbas, Imane; Goff, Jérémie Le; Verdin, Anthony; André, Véronique; Lafargue, Paul-Eric; Hachimi, A.; Cazier, Fabrice; Sichel, François; Shirali, Pirouz; Garçon, Guillaume

doi:10.1016/j.canlet.2008.04.044

Cited by 97 publications

(64 citation statements)

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“…Fe) (Carter et al 1997 ;Hutchison et al 2005 ;Roig et al 2013 ';Schins et al 2004 ), organics (i.e. polycyclic aromatic hydrocarbons or PAHs) (Billet et al 2008 ) and endotoxins (Becker et al 2003 ;Longhin et al 2013 ;Oberdorster et al 2000 ; in the PM seem to be involved in this process (Donaldson et al 2003 ;Schwarze et al 2006 ;Sorensen et al 2003 ). Moreover, although secondary inorganic aerosols show less toxic activity when tested under controlled laboratory conditions, epidemiological studies demonstrate significant associations between sulphates and nitrates and various health outcomes.…”

Section: Introductionmentioning

confidence: 99%

“…The PM10 fraction has been associated with pro-inflammatory and cytotoxic effects (Gualtieri et al 2010 ;Hetland et al 2005 ), and the PM2.5 fraction has been associated primarily with a higher genotoxic potential (Billet et al 2008 ) also related to higher PAH and metal contents. Moreover, positive relationships were found between the formation of ROS and the induction of DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Alessandria¹,

Schilirò²,

Degan³

et al. 2014

Environ Sci Pollut Res

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Alessandria¹,

Schilirò²,

Degan³

et al. 2014

Environ Sci Pollut Res

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“…The colony-forming efficiency assay was also performed in parallel with the CTA to calculate the number of cells surviving the chemical treatment, as well as to detect possible differences in the toxic response to environmental mixtures (Figure 1). Previously reported data strongly support the use of in vitro alternative methods for the assessment of toxicity, genotoxicity or transforming potential of environmental mixtures extracted from particulate matter [19][20][21][22][23]. It has been reported that PM exhibits different toxicological characteristics according to the pollution source [20,23,24].…”

Section: Cell Transformation Assaymentioning

confidence: 77%

Alternative Testing Methods for Predicting Health Risk from Environmental Exposures

et al. 2014

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Alternative methods to animal testing are considered as promising tools to support the prediction of toxicological risks from environmental exposure. Among the alternative testing methods, the cell transformation assay (CTA) appears to be one of the most appropriate approaches to predict the carcinogenic properties of single chemicals, complex mixtures and environmental pollutants. The BALB/c 3T3 CTA shows a good degree of concordance with the in vivo rodent carcinogenesis tests. Whole-genome transcriptomic profiling is performed to identify genes that are transcriptionally regulated by different kinds of exposures. Its use in cell models representative of target organs may OPEN ACCESS Sustainability 2014, 6 5266 help in understanding the mode of action and predicting the risk for human health. Aiming at associating the environmental exposure to health-adverse outcomes, we used an integrated approach including the 3T3 CTA and transcriptomics on target cells, in order to evaluate the effects of airborne particulate matter (PM) on toxicological complex endpoints. Organic extracts obtained from PM 2.5 and PM 1 samples were evaluated in the 3T3 CTA in order to identify effects possibly associated with different aerodynamic diameters or airborne chemical components. The effects of the PM 2.5 extracts on human health were assessed by using whole-genome 44 K oligo-microarray slides. Statistical analysis by GeneSpring GX identified genes whose expression was modulated in response to the cell treatment. Then, modulated genes were associated with pathways, biological processes and diseases through an extensive biological analysis. Data derived from in vitro methods and omics techniques could be valuable for monitoring the exposure to toxicants, understanding the modes of action via exposure-associated gene expression patterns and to highlight the role of genes in key events related to adversity.

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“…Ces spectres comportent environ 20 % de transition A > G, tout comme l'échantillon particulaire collecté dans le cadre de ce projet [35]. Par ailleurs, la faible fréquence des transversions G > T (6 %) confirme le rôle mineur des adduits encombrants à l'ADN, ce que suggérait l'absence d'adduits quantifiables sur les autoradiographies obtenues après exposition de cellules à l'échantillon particulaire collecté ou aux particules désorbées, lors d'une étude antérieure [36]. D'autre part, le test FASAY offre l'opportunité unique de permettre la comparaison directe des spectres mutationnels obtenus in vitro à ceux des tumeurs humaines, dans l'optique d'apporter des arguments de plausibilité biologique à la recherche des facteurs étiologiques des cancers.…”

Section: Discussionunclassified

Spectre mutationnel de TP53en réponse à une exposition in vitroà un aérosol atmosphérique particulaire PM2,5

Billet¹,

Garçon²,

Paget³

et al. 2012

Pollution Atmosphérique

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Spectre mutationnel de TP53 en réponse à une exposition in vitro à un aérosol atmosphérique particulaire PM 2,5 Mutational pattern of TP53 tumor suppressor gene in human lung cells exposed to air pollution PM 2.5Sylvain BILLET (1,2,3) , Guillaume GARÇON (1,2) , Vincent PAGET (1,2,3) , Anthony VERDIN (1,2) , Véronique ANDRÉ (3) , Natacha HEUTTE (3) , Pirouz SHIRALI (1, 2,*) et François SICHEL RésuméLa pollution atmosphérique particulaire fine (PM 2,5 ) constitue un des facteurs d'exposition contribuant à l'induction de certaines pathologies cardiopulmonaires, et notamment les cancers broncho-pulmonaires (CBP). Toutefois, la relation causale n'est pas suffisamment documentée quant aux mécanismes d'action potentiellement impliqués. En raison de son rôle de suppresseur de tumeurs, TP53 est muté dans la majorité des CBP. L'objectif de cette étude a donc été de rechercher les mutations susceptibles d'altérer la fonctionnalité de ce facteur de transcription dans un modèle de cellules pulmonaires épithéliales humaines (A549) exposées à la fraction fine d'un aérosol atmosphérique particulaire d'origine urbano-industrielle.Un échantillon particulaire de granulométrie fine a été collecté à Dunkerque par impaction en cascade. Sa caractérisation physico-chimique a révélé la présence de nombreux composés inorganiques et organiques, dont la toxicité intrinsèque est connue. La recherche des mutations altérant la fonctionnalité de la protéine P53 a été effectuée 72 h après l'exposition des cellules A549 à la concentration létale à 50 % (CL 50 , 118,60 μg/mL = 31,63 μg/cm 2

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Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM2.5) in human lung epithelial A549 cells

Cited by 97 publications

References 47 publications

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Alternative Testing Methods for Predicting Health Risk from Environmental Exposures

Spectre mutationnel de TP53en réponse à une exposition in vitroà un aérosol atmosphérique particulaire PM2,5

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