Genotoxicity and antileishmanial activity evaluation of Physalis angulata concentrated ethanolic extract

Nogueira, Renata Campos; Rocha, Vinícius Pinto Costa; Nonato, Fabiana Regina; Tomassini, Therezinha Coelho Barbosa; Ribeiro, Ícaro José Santos; Santos, Ricardo Ribeiro dos; Soares, Milena Botelho Pereira

doi:10.1016/j.etap.2013.10.013

Cited by 28 publications

(20 citation statements)

References 23 publications

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“…Despite the immunomodulatory potential or these compounds, the purification of physalins is an expensive, time-consuming, and low-yielding process [17]. Because of these limitations, the use of a standardized EEPA becomes an attractive and viable alternative.…”

Section: In Vitro and In Vivo Immunomodulatory Activity Of Physalis Amentioning

confidence: 99%

“…Because of these limitations, the use of a standardized EEPA becomes an attractive and viable alternative. The standardized ethanolic extract has proven to be non-mutagenic and is effective against different Leishmania species [17]. In addition, in a model of acute Chagas disease, treatment with EEPA decreased blood parasitemia and increased survival of infected mice [18].…”

Section: In Vitro and In Vivo Immunomodulatory Activity Of Physalis Amentioning

confidence: 99%

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In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

et al. 2020

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The need for new immunomodulatory drugs is due to the side effects associated with the prolonged use of the currently used immunomodulatory drugs. In this context, the present work aimed to investigate the immunomodulatory effect of an ethanolic concentrated extract from Physalis angulata. The cytotoxicity of samples was determined using peritoneal macrophages though the Alamar Blue assay. The immunomodulatory activity of the ethanolic extract from P. angulata on activated macrophages was determined by measurement of nitrite and cytokine production. The immunosuppressive effects of the ethanolic extract from P. angulata was evaluated on lymphocyte proliferation and cytokine production. The effects of the extract on cell cycle progression and cell death on lymphocytes were evaluated by flow cytometry. Lastly, the ethanolic extract from P. angulata was tested in vivo in toxicological tests and in models of peritonitis and delayed-type hypersensitivity response. The ethanolic extract from P. angulata decreased nitrite, interleukin-6, interleukin-12, and TNF-α production by activated macrophages without affecting the cell viability. In addition, the ethanolic extract from P. angulata inhibited lymphoproliferation and the secretion of interleukin-2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion by activated splenocytes. Flow cytometry analysis in lymphocyte cultures showed that treatment with the ethanolic extract from P. angulata induces cell cycle arrest in the G1 phase followed by cell death by apoptosis. Moreover, mice treated with the extract from P. angulata at 100 or 200 mg/kg did not show signs of toxicity or alterations in serum components. Finally, the ethanolic extract from P. angulata significantly reduced neutrophil migration and reduced paw edema in bovine serum albumin-induced the delayed-type hypersensitivity response model. Our results demonstrate the potential of the ethanolic extract of P. angulata as an alternative for the treatment of immune-inflammatory diseases.

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Section: In Vitro and In Vivo Immunomodulatory Activity Of Physalis Amentioning

confidence: 99%

Section: In Vitro and In Vivo Immunomodulatory Activity Of Physalis Amentioning

confidence: 99%

In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

et al. 2020

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“…[4][5][6][7][8][9] Besides, pharmacological researches on extracts and isolated compounds from the plant showed anti-inflammatory, anti-allergic, anti-nociceptive, anti-parasitic, anti-bacterial, antioxidant, diuretic, immunomodulatory, and cytotoxic activities. [9,[10][11][12][13][14] . In the present study, we described the isolation and chemical structural elucidation of three withanolides from the dichloromethane extract of the whole plant of P. angulata (figure 1).…”

Section: Introductionmentioning

confidence: 99%

Withanolides from the whole plant of Physalis angulata and their anti‐inflammatory activities

Yến

Cuong

Dat

et al. 2019

Vietnam Journal of Chemistry

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From the dichloromethane extract of the whole plant of P. angulata growing in Vietnam, three withanolides including physagulin L (1), physagulin M (2), and withaminimin (3) were isolated by using various chromatography methods. Their chemical structures were determined by detailed analysis of 1D and 2D-NMR data and comparison with reported data. All isolated compounds were tested for their inhibitory activities against NO production in LPS-stimulated RAW 264.7 macrophages. The results showed that compound 3 had a weak anti-inflammatory activity against NO production in RAW 264.7 macrophages with IC50 value of 69.6±4.5 μM.

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“…Estudos fitoquímicos revelaram a presença de flavonoides e fisalinas, sendo que as fisalinas agem sobre diferentes espécies de Leishmania (GUIMARÃES et al, 2010) onde é possível utilizar o extrato etanólico de caules que, além de apresentar efeito contra Leishmania não é mutagênico (NOGUEIRA et al, 2013). Extratos etanólicos dos frutos de P. angulata apresentam atividade antibacteriana, segundo Lopes et al (2006).…”

Section: Introductionunclassified

Tipos de estacas e uso de AIB na propagação vegetativa de fisális

et al. 2015

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Resumo:Com o presente trabalho objetivou-se avaliar a propagação vegetativa de Physalis angulata L. utilizando-se diferentes tipos de estacas e concentrações de ácido 3-indolbutírico (AIB). Foram avaliadas mudas produzidas a partir de estacas coletadas em plantas matrizes na fase reprodutiva. O delineamento experimental foi em blocos ao acaso, em esquema fatorial 2 x 5, sendo os fatores constituídos por dois tipos de estacas (basal e mediana) e cinco concentrações de AIB (0, 400, 800, 1.200 e 1.600 mg L -1 ), com quatro repetições e cinco estacas por parcela. Aos 20 e 40 dias após o plantio (DAP) foram avaliados:o número de brotações, número de folhas e comprimento da brotação. Aos 60 DAP foram avaliadas: percentagem de sobrevivência, número e comprimento da maior raiz, massa seca de folhas e massa seca total. Aos 20 DAP não foram encontradas diferenças significativas para nenhuma das variáveis analisadas, sendo que aos 40 DAP verificou-se efeito significativo para concentração de AIB para número de folhas. Aos 60 DAP verificouse que é dispensável o uso de AIB, em virtude da pouca eficiência do regulador nas variáveis avaliadas. Recomenda-se a produção de mudas de fisális por estaquia sem utilização do AIB. Palavras-chave: Physalis angulata L. Produção de mudas. Propagação vegetativa.Abstract: The aim of this work was to evaluate vegetative propagation in Physalis angulata L. using different types of cuttings and concentrations of IBA. Seedlings, produced from cuttings collected from parent plants in the reproductive phase, were evaluated. The experimental design was of randomised blocks in a 2 x 5 factorial scheme, with the factors consisting of two types of cutting (basal and medial) and five concentrations of IBA (0, 400, 800, 1,200 and 1,600 mg L -1 ) with four replications and five cuttings per lot. Evaluations were made at 20 and 40 days, of the number of shoots, number of leaves and length of shoots. At 60 days the percentage of survival, the number of roots and length of the largest root, leaf dry weight and total dry weight were evaluated. At 20 days no significant differences were found for any of the variables under analysis, whereas at 40 days, there was a significant effect on the number of leaves for the concentration of IBA only. At 60 days it was found that the use of IBA can be dispensed with, due to the low efficiency in regulating the variables under evaluation. Production of physalis seedlings by cutting is recommended without the use of IBA.

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Genotoxicity and antileishmanial activity evaluation of Physalis angulata concentrated ethanolic extract

Cited by 28 publications

References 23 publications

In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

Withanolides from the whole plant of Physalis angulata and their anti‐inflammatory activities

Tipos de estacas e uso de AIB na propagação vegetativa de fisális

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