Genotoxicity and mutagenicity of Rosmarinus officinalis (Labiatae) essential oil in mammalian cells in vivo

Maistro, Edson Luis; Mota, S. F.; Lima, Elisiane; Bernardes, B. M.; Goulart, F. C.

doi:10.4238/vol9-4gmr857

Cited by 35 publications

(31 citation statements)

References 25 publications

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“…The appearance of micronuclei may be due to the formation of lagging acentric chromosomes or chromatid fragments caused by unrepaired or misrepaired DNA breaks during anaphase, or may be due to the malsegregation of the whole chromosomes at anaphase (Fenech et al 2011). The formation of micronuclei was reported by other studies which showed the genotoxic effect of the aqueous extracts from Arum maculatum and Rubia cordifolia (Abderrahman 2004), and the genotoxic effect of the essential oil from Rosmarinus officinalis (Maistro et al 2010) on mice bone marrow cells. The aqueous extract of Catha edulis was also shown to induce micronuclei formation in the cells of Allium cepa root tips (Kabarity and Malallah 1980).…”

Section: Discussionmentioning

confidence: 75%

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Abderrahman

Shbailat

2014

Caryologia

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Artemisia herba-alba is a medicinal plant that is commonly used in folk remedies in the Middle East. Despite its wide use, little is known about whether or not it is genotoxic and/or cytotoxic to mammalian cells. Here we investigated the genotoxic and cytotoxic effects of the ethanolic plant extract on mice cells at different dose concentrations (125, 250, 375, and 500 μg ml ) and for different exposure times (12, 24, 48, and 72 h). We found that at all exposure times, treatment with high dose concentrations (375 and 500 μg ml ) of A. herba-alba extract significantly reduced cell division in mice bone marrow cells compared to that in the control cells. Also, it induced sister chromatid exchanges and micronuclei formation in bone marrow and peripheral blood cells, respectively. Moreover, high dose concentrations significantly reduced cell viability in bone marrow cell cultures compared to that in the controls. The IC 50 values were 484.38, 513.33, and 490.39 μg ml −1 after 24, 48, and 72 h of exposure, respectively. Our findings reveal that A. herba-alba extract has both genotoxic and cytotoxic effects on mammalian cells, particularly at high concentrations. Therefore, we suggest that the plant extract should not be used at high concentrations in folk medicine.

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Section: Discussionmentioning

confidence: 75%

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Abderrahman

Shbailat

2014

Caryologia

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“…The latter correspond probably to spontaneous oxidative DNA lesions. We suppose that DNA protective effect of higher RO concentrations could be camouflaged to a certain extent by their simultaneous mild pro-oxidative activity (Maistro, Mota, Lima, Bernardes, & Goulart, 2010).…”

Section: Discussionmentioning

confidence: 99%

Essential rosemary oil protects testicular cells against DNA-damaging effects of H2O2 and DMNQ

et al. 2011

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“…These results mean that not only cytotoxic, but also mutagenic activity of LA oil is related to the presence of linalyl acetate. Maistro et al [6] investigated genotoxicity of RO essential oil using three tests (SCGE, micronucleus and chromosome aberration tests) in liver cells and peripheral blood cells collected from Swiss mice and bone marrow cells isolated from Wistar rats. The authors concluded that this essential oil provokes genotoxic effects in experimental animals when administered orally (once 300, 1000 or 2000 mg/kg).…”

Section: Discussionmentioning

confidence: 99%

“…Today, it is grown in nearly all parts of the Mediterranean region and Asia. LA and RO are used for many purposes including medicine, food industry, dyeing, repellents, fragrances and cosmetics [6][7][8]. The continuing and growing human exposure to natural products has led to an enhancement of the scientific interest about their biological effects.…”

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confidence: 99%

Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems

Horváthová¹,

Kozics²,

Melušová³

et al. 2017

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Knowledge of biological properties of natural compounds allows to understand their therapeutic value, efficacy and security. We investigated: composition of Lavandula angustifolia (LA) and Rosmarinus officinalis (RO) extracts, their antioxidant capacity, cytotoxicity and genotoxicity, their DNA-protective potential against DNA damage induced in hamster V79 cells by several genotoxins or in plasmid DNA by Fe2+ ions and activity of antioxidant enzymes in cells treated with these extracts. Higher cytotoxicity, observed at higher concentrations of extracts, was accompanied by the increased level of single-strand (ss) DNA breaks as well as formamidopyrimidine DNA glycosylase (Fpg) sensitive sites. LA and RO extracts were able to protect DNA of hamster cells as well as plasmid DNA against ss DNA breaks induced by genotoxins and Fe2+. LA extract mildly increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), while RO extract decreased the activity of SOD, but increased the activity of CAT and GPx. Cell-free tests confirmed antioxidant activity of both extracts. The biological properties of LA and RO extracts showed that they could have a positive impact on human health.

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Genotoxicity and mutagenicity of Rosmarinus officinalis (Labiatae) essential oil in mammalian cells in vivo

Cited by 35 publications

References 25 publications

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Essential rosemary oil protects testicular cells against DNA-damaging effects of H2O2 and DMNQ

Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems

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