Genotoxicity assessment of Pyungwi-san (PWS), a traditional herbal prescription

Shin, In Sik; Seo, Chang Seob; Ha, Hye Kyung; Lee, Mee Young; Huang, Dae Sun; Huh, Jung Im; Shin, Hyeun‐Kyoo

doi:10.1016/j.jep.2010.10.050

Cited by 21 publications

(19 citation statements)

References 25 publications

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“…The chrosomal preparations were done according to the method described earlier [20] . The frequency of the cells with structural and numeric chromosomal aberrations was scored in 100 well spread metaphase for each dose.…”

Section: In Vitro Chromosomal Aberration Test In Chinese Hamster Lungmentioning

confidence: 99%

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Evaluation of genotoxicity of Trois through Ames and In vitro chromosomal aberration tests

Chaudhary

Payasi

2013

Asian Pacific Journal of Tropical Biomedicine

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Section: In Vitro Chromosomal Aberration Test In Chinese Hamster Lungmentioning

confidence: 99%

“…Ha et al (2011) have studied the genotoxicity assessment of a herbal formula, Ojeok-san and have found that Ojeok-san is toxicologically safe on genotoxicity studies.…”

Section: Related Reportsmentioning

confidence: 99%

Evaluation of genotoxicity of Trois through Ames and In vitro chromosomal aberration tests

Chaudhary

Payasi

2013

Asian Pacific Journal of Tropical Biomedicine

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“…Therefore, it is very significant for scientists to screen active compounds from plants and synthesized new derivatives with similar molecular structures as potential anti-tumor drugs or pro-drugs [1][2][3]. As a common active ingredient of many Chinese herbal medicines [4,5], flavonoids reveal little side effects in clinical research [6,7].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Anti-Tumor Activity of Novel Aminomethylated Derivatives of Isoliquiritigenin

Zhang

Wang

et al. 2014

Molecules

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A series of new aminomethylated derivatives of isoliquiritigenin was synthesized. The structures of the compounds were confirmed by IR, MS, NMR, 13 C-NMR and elemental analyses. Cytotoxic activities of these derivatives towards the human prostatic cell line PC-3, human mammary cancer cell line MCF-7 and human oophoroma cell line HO-8910 in vitro were tested. The IC50 values showed cytotoxic activities of some of these new derivatives were relatively strong. Furthermore, tumor growth inhibition in vivo of aminomethylated derivatives of isoliquiritigenin 15 was superior to that of isoliquritigenin and reached inhibition rates of 71.68%. The detailed synthesis, spectroscopic data, biological and pharmacologicalactivities of the synthesized compounds were provided.

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“…In addition, PWS has been shown to provide biological benefits, such as protecting from gastrointestinal mucosal damage and possessing an anti-oxidative activity. [91011] In this study, we develop and validate an HPLC with diode array detection (HPLC-DAD) method and MS (LC-MS/MS) for quantitative determination of five compounds in PWS: (1) Naringin, (2) hesperidin, (3) glycyrrhizin, (4) atractylenolide III, (5) magnolol. These compounds were selected as major compound of consisted herbs: Naringin and hesperidin from C. Pericarpium , glycyrrhizin from G. Radix , atractylenolide from A. Rhizoma , and magnolol from M. Cortex .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of five major compounds in the traditional medicine Pyeongwee-San by high performance liquid chromatography-diode array detection and liquid chromatography-mass spectrometry/mass spectrometry

Lee

Weon²,

Yun³

et al. 2014

Phcog Mag

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Background:Pyeongwee-San (PWS) has been widely used for treating acute gastritis, chronic, and gastritis.Objective:In this paper, simultaneous determination of five compounds (naringin, hesperidin, glycyrrhizin, atractylenolide III, and magnolol) from traditional medicine PWS using the high performance liquid chromatography (HPLC) was established for quality control.Materials and Methods:Optimum separations were obtained with a SHISEIDO C18 reverse-phase column by gradient elution with 0.1% Trifluoroacetic acid (TFA) water-acetonitrile as the mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 205 nm and 250 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test.Results:The calibration curves were linear over the established range with R2 > 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.09 to 0.43 and 0.27 to 1.29 μg/mL. The method exhibited intra-day and inter-day precision range between 0.01-1.86% and 0.04-0.35% respectively. The recoveries of five compounds in PWS were in the range between 93.18-106.40%, and 0.20-1.51%. The application of this method was identified through the successful analysis of five compounds in 12 batches of PWS. In addition, identification of five compounds was confirmed by a liquid chromatography method and mass spectrometry.Conclusion:The HPLC method was could be accomplished to the quality control and stable experiment for the preparations consisted of five major compounds.

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Genotoxicity assessment of Pyungwi-san (PWS), a traditional herbal prescription

Cited by 21 publications

References 25 publications

Evaluation of genotoxicity of Trois through Ames and In vitro chromosomal aberration tests

Evaluation of genotoxicity of Trois through Ames and In vitro chromosomal aberration tests

Synthesis and Anti-Tumor Activity of Novel Aminomethylated Derivatives of Isoliquiritigenin

Simultaneous determination of five major compounds in the traditional medicine Pyeongwee-San by high performance liquid chromatography-diode array detection and liquid chromatography-mass spectrometry/mass spectrometry

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