Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice

Fahmy, Maha A.; Abdalla, E. F.

doi:10.1002/(sici)1099-1263(1998090)18:5<301::aid-jat503>3.0.co;2-g

Cited by 19 publications

(7 citation statements)

References 14 publications

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“…[14] Profenofos has been reported to induce different types of chromosomal aberrations in the germ cells of mice. [15] It also induced apoptosis, necrosis, chromatid breaks and single-strand breaks in cultured human peripheral blood lymphocytes. [16]…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Bhinder

Chaudhry

2014

Toxicol Int

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Objectives:In this study we have evaluated the genotoxic potential of pesticides acephate and profenofos by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as experimental model.Material and Methods:Second instar larvae were treated with LC20 of each pesticide for 24 h and induced mutations in the sequence of mitochondrial 16S rRNA gene were studied from restriction patterns generated with PacI and PsiI restriction endonucleases.Results:Variations in the number and size of digested fragments were recorded from treated individuals compared with controls showing that the restriction enzymes created a cut at different locations. In addition, sequences of the 16S gene from control and treated individuals were also used to confirm the RFLP patterns. From the sequence alignment data, it was found that mutations caused the destruction and generation of restriction sites in the gene sequence of treated individuals.Conclusion:This study indicates that both the pesticides had significant potential to induce mutations in the 16S gene of Culex quinquefasciatus.

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Section: Resultsmentioning

confidence: 99%

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Bhinder

Chaudhry

2014

Toxicol Int

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“…[15] Profenofos has been reported to induce different types of chromosomal aberrations in the germ cells of mice. [16] It also induced apoptosis, necrosis, chromatid breaks, and single-strand breaks in cultured human peripheral blood lymphocytes. [17]…”

Section: Resultsmentioning

confidence: 99%

Mutagenicity assessment of organophosphates using polymerase chain reaction-restriction fragment length polymorphism assay

Bhinder¹,

Chaudhry²

2013

Toxicol Int

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Objectives:In this study we have evaluated the mutagenicity of organophosphate pesticides acephate, chlorpyrifos, and profenofos using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as an experimental model.Materials and Methods:Second instar larvae were treated with LC20 of each pesticide for 24 h and mutations induced in the sequence of mitochondrial COII gene (690bp) were studied from restriction patterns generated with AluI, PacI, and PsiI restriction endonucleases.Results:Variations in the number and size of digested fragments were recorded from treated individuals compared with controls showing that the restriction enzymes created a cut at different locations. In addition, sequences of COII gene from control and treated individuals were also used to confirm the RFLP patterns. From the sequence alignment data, it was found that mutations caused the destruction and generation of restriction sites in the gene sequence of treated individuals.Conclusion:This study indicates that all the three pesticides had potential to induce mutations in the normal sequence of COII gene and also advocates the use of PCR-RFLP assay as an efficient, rapid, and sensitive technique to detect mutagenicity of pesticides.

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“…Bu konuya yönelik daha önce yapılmış çeşitli çalışmalar bulunmaktadır. Bu çalışmalardan bir kaçında; profenofosun farelerin spermatogoniumlarında, sperm ve kemik iliği hücrelerinde kromozomal bozukluklara yol açtığı [24,27], sıçanların polikromatik eritrositlerde mikronükleus frekansını artırdığı [5], insan periferal lenfosit hücrelerinde ise kromozomal kırıklara, gaplara ve DNA hasarlarına neden olduğu [6] görülmüştür. Bu araştırmacılara göre, profenofosun toksik ve genotoksik etkileri onun oksidatif stresi artırıcı özelliklerinden kaynaklanmaktadır.…”

Section: Discussionunclassified

Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

Kasimoğlu¹,

Uysal²

2016

CFD

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Özet. Bu çalışmada, profenofos insektisitinin genotoksik etkileri, Drosophila melanogaster'de somatik mutasyon ve rekombinasyon testi (SMART) ve insan periferal lenfosit hücrelerinde mikronükleus (MN) testi ile araştırılmıştır. Bu maddenin olası genotoksik etkilerinin giderilebilmesi için de kuşburnu (Rosa canina) bitkisine ait su ve etanol ekstreleri kullanılmıştır.SMART için D.melanogaster'in kanat preparatları incelendiği zaman, profenofosun artan konsantrasyonuna (0,025, 0,05, 0,075 ve 0,1ppm) bağlı olarak normal kanat fenotipinde mutasyon frekansının arttığı özellikle en yüksek uygulama grubunda (0,1 ppm) bu artışın pozitif etkili (+) olduğu gözlenmiştir (P<0,05). Serrat kanat fenotipinde ise mutasyon frekansındaki artış tüm uygulama gruplarında önemsiz etkili (i) bulunmuştur (P>0,05). Kuşburnu bitkisinin su (RCsu) ve etanol (RCeta) ekstreleri, profenofosun en yüksek konsantrasyonu (0,1 ppm) ile birlikte uygulandığı zaman (profenofos+ RCsu/RCeta) mutasyon frekansındaki artışın azaldığı ve bunun da istatistiksel olarak önemli olduğu gözlenmiştir (P<0,05). The Usage of Rosa canina L. Extracts As a Natural Anti-Genotoxic Agents Against the Genotoxicity of Profenofos on Somatic Cells by Different Test SystemsAbstract. In this study, the genotoxic effects of profenofos insecticide were investigated with the somatic mutation and recombination test (SMART) in Drosophila melanogaster and the micronucleus test (MN) in human peripheral lymphocytes. The water and ethanol extracts of rosehip plants (Rosa canina) were used to remove possible genotoxic effects of this substance.When the wing preparates of D.melanogaster were examined for the SMART, it was observed that mutation frequency in normal wing phenotype increased depending on doses of profenofos (0,025, 0,05, 0,075 ve 0,1ppm) and this increase was positive effect (+) in the highest application group (0,1 ppm), especially. The increase in mutation frequency of the serrate wings phenotype was found insignificant (i) in the all application groups (P>0,05). When the water (RCwtr) and ethanol (RCeth) extracts of the rosehip plants were applied together with the highest application concentration of profenofos (profenofos+ RCwtr/RCeth), it was observed that the increase in the mutation frequency decreased and this decrease was statistically significant (P<0,05).In this study, the mutation frequency increased and nuclear division index (NBI) decreased in the human lymphocyte cells with in vitro profenofos application in the different concentration were showed (P<0,05). But as the MN frequencies decreased, NBI values approached to control group with profenofos+RCwtr and RCeth applications (P<0,05).

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Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice

Cited by 19 publications

References 14 publications

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Mutagenicity assessment of organophosphates using polymerase chain reaction-restriction fragment length polymorphism assay

Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

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