Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo

Dobrzyńska, Małgorzata M.; Gajowik, Aneta; Radzikowska, Joanna; Lankoff, Anna; Dušinská, Maria; Kruszewski, Marcin

doi:10.1016/j.tox.2013.11.012

Cited by 127 publications

(85 citation statements)

References 59 publications

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“…Another follow-up study found no significant increase in multinucleated polychromatic erythrocytes in bone marrow (Kim et al, 2011). However, it should be noted that the presence of silver in bone marrow was not determined in the study and data for intravenous injection show clear genotoxic effects of silver NPs (Dobrzynska et al, 2014). A study investigating the reversibility of lung function showed impairment after 12 weeks of exposure employed silver NPs with concentrations of 6.6 Â 10 5 to 3.2 Â 10 6 particles/cm 3 (Song et al, 2013).…”

Section: Discussionmentioning

confidence: 76%

Comparative modeling of exposure to airborne nanoparticles released by consumer spray products

2015

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Consumer exposure to sprays containing nano-objects is a continuing concern as a potential health hazard. One potential hazard has been formulated in the overload hypothesis. It describes a volume fraction of the macrophages that is occupied by deposited nanoparticles that leads to reduced macrophage mobility. Subsequent chronic inflammation may then lead to severe health consequences including cancer. To calculate lung deposition of spherical particles, the Multiple-Path Particle Dosimetry (MPPD) model (ARA, Albuquerque, NM) provides different kinds of lung models and age settings. Using the MPPD v 2.11 software, we modeled several consumer-related exposure scenarios. Different body orientations and age groups were investigated. Moreover, a number of materials representing different densities were used, and the exposure calculated using MPPD is compared to the hazard derived from the overload hypothesis. Conditions leading to macrophage overload were found for exposures to high particle doses for prolonged times and repeated exposure. Such conditions are unlikely in the context of regular consumer exposure. The overload hypothesis assumes the particles to be inert and biopersistent, a condition that currently lacks a clear regulatory definition and is valid only for a few selected materials. Furthermore, because of material-specific effects and the possibility of surface adsorption of hazardous chemicals, nano-objects in propellant sprays remain of concern for consumer health.

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Section: Discussionmentioning

confidence: 76%

Comparative modeling of exposure to airborne nanoparticles released by consumer spray products

2015

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“…Por otro lado, las NP de TiO 2 causaron la mayor disminución de la expresión de los genes estudiados. Este efecto estaría relacionado a una disminución de la carga linfocitaria en los roedores (19) . Park et al (20) determinaron que las NP de TiO 2 producen daño pulmonar, incluyendo citotoxicidad, estrés oxidativo, respuestas inflamatorias, proliferativas e histopatológicas en modelos murinos.…”

Section: Discussionunclassified

“…En el presente estudio se usó un rango mayor de concentración de esta NP debido a que previamente se determinó que en estas concentraciones se puede evidenciar efecto citotóxico. Este mismo hecho también se da en modelos en ratas (19) . La exposición a las NP de SiO 2 conlleva a la mayor disminución del GM-CSF en comparación de las otras NP, este efecto podría comprometer la barrera antibacteriana a nivel celular por la alteración de los neutrófilos y monocitos (13) .…”

Section: Referencias Bibliográficasunclassified

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Zarria-Romero

Osorio

Pino

et al. 2017

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Evaluar el efecto de las nanopartículas de ZnO, TiO 2 y SiO 2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito -macrófago (GM-CSF) en Mus musculus. Materiales y métodos. Se extrajo médula ósea roja de cinco roedores (Balb/c) para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO 2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas deTiO 2 , 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados. Las nanopartículas de ZnO y SiO 2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO 2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente), con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO 2 (91%). Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones. Las nanopartículas de ZnO, SiO 2 y TiO 2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón. Palabras clave: Nanopartículas; Interleucinas; Expresión génica; Mus musculus (fuente: DeCS BIREME). EFFECT OF THE INDUSTRIAL NANOPARTICLES TiO 2 , SiO 2 and ZnO ON CELL VIABILITY AND GENE EXPRESSION IN RED BONE MARROW OF Mus musculus ABSTRACT Objectives.To evaluate the effect of ZnO, TiO 2 and SiO 2 nanoparticles on cell viability and expression of the interleukin 7, interleukin 3, and granulocyte-macrophage colony stimulating factor (GM-CSF) genes in Mus musculus. Materials and methods. Red bone marrow was extracted from five Balb/c mice for the analysis of cell viability using the MTT test. The mice were divided into two groups of five each: one group was inoculated intraperitoneally with 0.5, 1.0, 2.5, 5.0, and 10 mg/kg of ZnO and SiO 2 nanoparticles, respectively, and the other group was inoculated with 5.0, 10.0, 15.0, 20.0, and 25 mg/kg of TiO 2 nanoparticles, respectively. Thirty hours later, RNA was extracted from the red bone marrow of the mice in both groups for gene expression analysis using quantitative PCR and RT-PCR. Results. ZnO and SiO 2 nanoparticles reduced cell viability in a dose-dependent manner by 37% and 26%, respectively, starting at a dose of 1 mg/kg. TiO 2 nanoparticles at 5 mg/kg and 10 mg/kg reduced the gene expression of interleukins 7 and 3 by 55.3% and 70.2%, respectively, and SiO 2 nanoparticles caused the greatest decrease (91%) in the expression of GM-CSF. ZnO nanoparticles reduced the expression of GM-CSF starting at doses of 20 mg/kg and 25 mg/kg. Conclusions: ZnO, SiO 2 and TiO 2 nanoparticles affect cell viability and gene expression in the mo...

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“…Therefore a logical application of Ag NP modified/ based materials is in medical practice. Ag NPs are used for surface modification of medical devices such as catheters, dental materials, bandage materials for wound and burn treatment (Asharani et al, 2009;Chopra, 2007;Dobrzyń ska et al, 2014;Greulich et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

Tománková

Hořáková

Harvanová

et al. 2015

Food and Chemical Toxicology

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Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage.

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Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo

Cited by 127 publications

References 59 publications

Comparative modeling of exposure to airborne nanoparticles released by consumer spray products

Comparative modeling of exposure to airborne nanoparticles released by consumer spray products

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

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