Genotoxicity of source, treated and distributed water from four drinking water treatment plants supplied by surface water in Sardinia, Italy

Feretti, Donatella; Acito, Mattia; Dettori, Marco; Ceretti, Elisabetta; Fatigoni, Cristina; S, Posadino; Zerbini, Ilaria; Villarini, Milena; Moretti, Massimo; Castiglia, Paolo; Azara, Antonio

doi:10.1016/j.envres.2020.109385

Cited by 25 publications

(16 citation statements)

References 29 publications

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“…58 Some other studies investigated mutagenicity, aryl hydrocarbon receptor activation in detecting dioxin-like activities, glucocorticogenicity and genotoxicity. 42,47,51,[59][60][61][62][63][64][65][66][67] While these are specic endpoints that have been somewhat legislated for in biomonitoring of water quality, the list of endpoints that are measurable is open-ended. Chemicals modifying biological processes do so at the molecular level through activation of specic proteins that may be modied by perturbed gene expression.…”

Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

“…Assessment of changes to the genetic make-up of aquatic organisms is one of the debilitating effect of chemical contaminants, which is why genotoxicity assessment has been widely investigated globally. 66,73,[141][142][143][144][145][146][147][148][149] Genotoxicity was mainly evaluated by Ames, comet and p53-based reporter assay. However, whole organism assessments for DNA damage were also common.…”

Section: Other Measured Endpoints In All Matricesmentioning

confidence: 99%

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Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

et al. 2021

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Section: Major Mode Of Actions and Endpointsmentioning

confidence: 99%

Section: Other Measured Endpoints In All Matricesmentioning

confidence: 99%

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

et al. 2021

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“…27 Estudios recientes muestran que el uso de agua desinfectada con dióxido de cloro muestra efectos mutagénicos en ensayos in vitro. 28 Dado que los productos clorinados presentan toxicidad en el organismo su ingesta no es recomendada. Por ello llama la atención la propuesta de su ingesta como una forma de prevenir o de tratar al COVID-19, que ha surgido como alternativa en esta pandemia del COVID-19.…”

Section: Exposición De Humanos Al Dióxido De Cloro Durante La Desinfección Del Agua De Consumounclassified

Ingesta de dióxido de cloro para la COVID-19

Gonzales

Vásquez‐Velásquez

2021

spmi

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Durante muchos años, el dióxido de cloro ha sido utilizado por su principal característica como desinfectante, abarcando diversas áreas de aplicación como la sanitaria, alimenticia, industrial, etc. La evidencia reportada hasta la fecha cita diversos estudios en modelos animales, desde revisiones sobre la toxicocinética hasta la aplicación o usos del compuesto. Su poder como bactericida, virucida y eliminador microbiológico lo convirtió en una fuente de control y potabilización de aguas. La controversia generada actualmente es sobre el uso de este compuesto para el tratamiento y control de la COVID-19. Se ha evidenciado incongruencia con algunos resultados reportados y que su aplicación en pacientes positivos a SARS-CoV-2 o para prevenir COVID-19 puede tener efectos adversos, como un acrecentado número de intoxicaciones y, en otros casos, la muerte. En la presente revisión se detalla, a partir de la evidencia científica reportada, los efectos de este compuesto sobre la salud y la interacción con el virus SARS-CoV-2.

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“…First, the Ames test exposes the bacteria to high concentrations of a single test compound over a short period of time, usually 48 hours [20], as low concentrations of mutagen may result in the control and experimental groups having similar numbers of mutant colonies, leading to an ambiguous result [21]. However, likely human exposure to suspected mutagens is expected to be at low concentrations over a very long period of time, such as when drinking water is contaminated by a mixture of pollutants, each present in very small concentrations [22]. In assessing the safety of such water, it is generally assumed that effects of potential mutagens are both linearly related to concentration and additive, but whether these assumptions are correct is difficult to determine with the present technologies [23].…”

Section: Introductionmentioning

confidence: 99%

Yeast grown in continuous culture systems can detect mutagens with improved sensitivity relative to the Ames test

et al. 2021

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Continuous culture systems allow for the controlled growth of microorganisms over a long period of time. Here, we develop a novel test for mutagenicity that involves growing yeast in continuous culture systems exposed to low levels of mutagen for a period of approximately 20 days. In contrast, most microorganism-based tests for mutagenicity expose the potential mutagen to the biological reporter at a high concentration of mutagen for a short period of time. Our test improves upon the sensitivity of the well-established Ames test by at least 20-fold for each of two mutagens that act by different mechanisms (the intercalator ethidium bromide and alkylating agent methyl methanesulfonate). To conduct the tests, cultures were grown in small, inexpensive continuous culture systems in media containing (potential) mutagen, and the resulting mutagenicity of the added compound was assessed via two methods: a canavanine-based plate assay and whole genome sequencing. In the canavanine-based plate assay, we were able to detect a clear relationship between the amount of mutagen and the number of canavanine-resistant mutant colonies over a period of one to three weeks of exposure. Whole genome sequencing of yeast grown in continuous culture systems exposed to methyl methanesulfonate demonstrated that quantification of mutations is possible by identifying the number of unique variants across each strain. However, this method had lower sensitivity than the plate-based assay and failed to distinguish the different concentrations of mutagen. In conclusion, we propose that yeast grown in continuous culture systems can provide an improved and more sensitive test for mutagenicity.

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Genotoxicity of source, treated and distributed water from four drinking water treatment plants supplied by surface water in Sardinia, Italy

Cited by 25 publications

References 29 publications

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

Monitoring of emerging contaminants of concern in the aquatic environment: a review of studies showing the application of effect-based measures

Ingesta de dióxido de cloro para la COVID-19

Yeast grown in continuous culture systems can detect mutagens with improved sensitivity relative to the Ames test

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