Genotoxicity of vanadium pentoxide in Chinese hamster V79 cells

Zhong, Beilong; Gu, Z.W.; Wallace, W.E.; Whong, Wen‐Zong; Ong, T.

doi:10.1016/0165-1218(94)90118-x

Cited by 35 publications

(14 citation statements)

References 25 publications

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“…Furthermore, DNA-damaging properties of vanadium compounds have also been found in human and animal cells in vitro (Ivancsits et al 2002; Kleinsasser et al 2003; Migliore et al 1993; Ramirez et al 1997; Rojas et al 1996; Roldan and Altamirano 1990; Zhong et al 1994). …”

Section: Discussionmentioning

confidence: 99%

“…Although oxidative damage of DNA bases caused by V 2 O 5 is probably due to release of reactive oxygen species, the formation of MNs may be due to its aneugenic properties, which have been found in in vitro studies (Galli et al 1991; Roldan and Altamirano 1990; Zhong et al 1994) and are apparently caused by disturbances of microtubule assembly (Ramirez et al 1997). …”

Section: Discussionmentioning

confidence: 99%

“…Results of in vitro and animal studies indicate that the oxide causes formation of reactive oxygen species (Ingram et al 2003, 2007; Wang et al 2003; Zhang et al 2001) and aneugenic effects (Migliore et al 1993; Ramirez et al 1997; Zhong et al 1994) and interferes with DNA synthesis and repair (IARC 2006). Because DNA damage and aneugenic processes are known to play a role in the onset of human cancer (Duesberg et al 2005; Pitot 1986), evidence of genetic damage in exposed humans would support the assumption of increased cancer risks.…”

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confidence: 99%

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Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study

Ehrlich

Nersesyan

Hoelzl

et al. 2008

Environ Health Perspect

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BackgroundInhalative exposure to vanadium pentoxide (V2O5) causes lung cancer in rodents.ObjectiveThe aim of the study was to investigate the impact of V2O5 on DNA stability in workers from a V2O5 factory.MethodsWe determined DNA strand breaks in leukocytes of 52 workers and controls using the alkaline comet assay. We also investigated different parameters of chromosomal instability in lymphocytes of 23 workers and 24 controls using the cytokinesis-block micronucleus (MN) cytome method.ResultsSeven of eight biomarkers were increased in blood cells of the workers, and vanadium plasma concentrations in plasma were 7-fold higher than in the controls (0.31 μg/L). We observed no difference in DNA migration under standard conditions, but we found increased tail lengths due to formation of oxidized purines (7%) and pyrimidines (30%) with lesion-specific enzymes (formamidopyrimidine glycosylase and endonuclease III) in the workers. Bleomycin-induced DNA migration was higher in the exposed group (25%), whereas the repair of bleomycin-induced lesions was reduced. Workers had a 2.5-fold higher MN frequency, and nucleoplasmic bridges (NPBs) and nuclear buds (Nbuds) were increased 7-fold and 3-fold, respectively. Also, apoptosis and necrosis rates were higher, but only the latter parameter reached statistical significance.ConclusionsV2O5 causes oxidation of DNA bases, affects DNA repair, and induces formation of MNs, NPBs, and Nbuds in blood cells, suggesting that the workers are at increased risk for cancer and other diseases that are related to DNA instability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study

Ehrlich

Nersesyan

Hoelzl

et al. 2008

Environ Health Perspect

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“…However, a dose-related increase of endoreduplication and micronucleated cells containing CREST-positive micronuclei was observed, indicating an aneugenic effect (Zhong et al, 1994).…”

Section: In Vitro Studies In Eukaryotic Cellsmentioning

confidence: 99%

“…In Chinese hamster V79 cells, no induction of forward mutations at the hprt locus was observed after treatment with vanadyl sulfate (+4) (0.081-0.81 µg/mL, both in the presence or in the absence of metabolic activation (Galli et al, 1991), or after treatment with vanadium pentoxide (1-3 µg/mL) in the absence of metabolic activation (Zhong et al, 1994). In experiments without exogenous metabolic activation, ammonium metavanadate, at low noncytotoxic concentrations (up to 0.58 ng/mL), increased the frequency of mutations at the hprt locus in V79 cells, and, at higher concentrations (0.585 µg/mL), at the bacterial gpt gene in transgenic G12 cells (Cohen et al, 1992).…”

Section: In Vitro Studies In Eukaryotic Cellsmentioning

confidence: 99%

Opinion of the Scientific Panel on Dietetic products, nutrition and allergies [NDA] related to the Tolerable Upper Intake Level of Vanadium

2004

EFSA Journal

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Reprotoxic and genotoxic studies of vanadium pentoxide in male mice

Altamirano-Lozano

Álvarez‐Barrera

Basurto-Alcántara

et al. 1996

Teratog. Carcinog. Mutagen.

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Effects of vanadium pentoxide (V2O5) treatment on reproductive function and testicular DNA in male mice were investigated. These functions were evaluated with fertility rate, implants, resorptions, sperm counts, motility, and morphology. The DNA damage in individual testis cells was analyzed by single‐cell gel electrophoresis technique (COMET assay). V2O5 treatment resulted in a decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment. Vanadium treatment induced DNA damage depending on the dose in the testis cells that was expressed and detected as DNA migration in the COMET assay. The distribution of DNA migration among cells, a function of dose, revealed that the majority of cells of treated animals expressed more DNA damage than cells from control animals. It is concluded that vanadium pentoxide was a reprotoxic and genotoxic agent in mice. © 1996 Wiley‐Liss, Inc.

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Genotoxicity of vanadium pentoxide in Chinese hamster V79 cells

Cited by 35 publications

References 25 publications

Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study

Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study

Opinion of the Scientific Panel on Dietetic products, nutrition and allergies [NDA] related to the Tolerable Upper Intake Level of Vanadium

Reprotoxic and genotoxic studies of vanadium pentoxide in male mice

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