Genotoxicity testing of Cecropia obtusifolia extracts in two in vivo assays: The wing somatic mutation and recombination test of Drosophila and the human cytokinesis-block micronucleus test

Toledo, Varenka Martínez; Téllez, María Guadalupe Ordaz; Sortibrán, América Nitxin Castañeda; Andrade‐Cetto, Adolfo; Rodríguez-Arnáiz, Rosario

doi:10.1016/j.jep.2007.10.041

Cited by 17 publications

(9 citation statements)

References 18 publications

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“…21,22 Other works report a content of 0.2 mg g -1 aqueous extract. 23,24 In our analysis, higher chlorogenic acid contents were found for C. glaziovii (11.1 ± 0.42 mg g -1 extract) and C. pachystachya (27.2 ± 0.94 mg g -1 extract) than those reported for C. obtusifolia, according to Revilla-Monsalve and coworkers.…”

Section: Quantification and Validation Proceduressupporting

confidence: 47%

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Costa¹,

Ortmann²,

Schenkel³

et al. 2011

J. Braz. Chem. Soc.

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Um método eficiente e reprodutível por CLAE-DAD foi desenvolvido e validado para quantificação simultânea dos compostos fenólicos majoritários (ácido clorogênico, isoorientina, orientina e isovitexina) presentes nas folhas de duas espécies de Cecropia, C. glaziovii e C. pachystachya. Das folhas de C. glaziovii e C. pachystachya foram isolados os flavonoides C-glicosídeos isoorientina e isovitexina e identificados em ambas as espécies o ácido clorogênico (ácido 3-O-cafeoilquínico) e o flavonoide O-glicosídeo isoquercitrina. O flavonoide C-glicosídeo orientina foi isolado apenas da espécie C. pachystachya. O ácido clorogênico mostrou-se como composto majoritário em ambas as espécies analisadas (11,1 mg g -1 de extrato de C. glaziovii e 27,2 mg g -1 de extrato de C. pachystachya), e em relação aos flavonoides quantificados, isovitexina se apresentou como o flavonoide C-glicosídeo majoritário para C. glaziovii (4,6 mg g -1 extrato) e isoorientina majoritário para C. pachystachya (17,3 mg g -1 de extrato).An efficient and reproducible HPLC-DAD method was developed and validated for the simultaneous quantification of major compounds (chlorogenic acid, isoorientin, orientin and isovitexin) present in the leaves of two Cecropia species, C. glaziovii and C. pachystachya. From the leaves of C. glaziovii and C. pachystachya were isolated the C-glycosylflavones isoorientin and isovitexin and identified on both species chlorogenic acid (3-O-caffeoylquinic acid) and the O-glycosylflavonol isoquercitrin. The C-glycosylflavone orientin was isolated only from C. pachystachya. Chlorogenic acid was the major compound in both species (11.1 mg g -1 of extract of C. glaziovii and 27.2 mg g -1 of extract of C. pachystachya) and for the flavonoids quantified, isovitexin was the main C-glycosylflavonoid for C. glaziovii (4.6 mg g -1 of extract) and isoorientin the main one for C. pachystachya (17.3 mg g -1 of extract).

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Section: Quantification and Validation Proceduressupporting

confidence: 47%

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Costa¹,

Ortmann²,

Schenkel³

et al. 2011

J. Braz. Chem. Soc.

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“…The difference between the inhibitory action of AF on the arginase and the parasite may be due to the access of drugs to the glycosome, the organelle where arginase is located in the parasite (da Silva et al, 2012b). No genotoxic effects were associated with Cecropia obtusifolia (Toledo et al, 2008), which suggests the possibility of developing innovative non-toxic drugs.…”

Section: Discussionmentioning

confidence: 99%

Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement

et al. 2013

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The plant Cecropia pachystachya Trécul is widely used in Brazilian ethnomedicine to treat hypertension, asthma, and diabetes. Arginase is an enzyme with levels that are elevated in these disorders, and it is central to Leishmania polyamine biosynthesis. The aims of this study were to evaluate antileishmanial activity and inhibition of the arginase enzyme by C. pachystachya extracts, and to study changes in cellular organization using electron microscopy. The ethanol extract of C. pachystachya was tested on Leishmania (Leishmania) amazonensis promastigote survival/proliferation and arginase activity in vitro. Qualitative ultrastructural analysis was also used to observe changes in cell organization. The major bioactive molecules of the ethanol extract were characterized using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The ethyl acetate fraction of the ethanol extract diminished promastigote axenic growth/survival, inhibited arginase activity, and altered a mitochondrial kinetoplast DNA (K-DNA) array. The bioactive compounds of C. pachystachya were characterized as glucoside flavonoids. Orientin (9) (luteolin-8-C-glucoside) was the main component of the methanol-soluble ethyl acetate fraction obtained from the ethanol extract and is an arginase inhibitor (IC50 15.9 μM). The ethyl acetate fraction was not cytotoxic to splenocytes at a concentration of 200 μg/mL. In conclusion, C. pachystachya contains bioactive compounds that reduce the growth of L. (L.) amazonensis promastigotes, altering mitochondrial K-DNA arrangement and inhibiting arginase.

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“…The lack of genotoxic activity observed for the extracts were consistent with the results obtained in other studies. Indeed, acute treatment of standard Drosophila larvae with basal biotransformation activity (ST cross) or a variant with increased CYP450-dependent bioactivation capacity (HB cross) did not result in genotoxicity (Téllez et al, 2007;Toledo et al, 2008). It is well-established that the electrophilic compounds generated by hydrogen peroxide produce genetic damage (Corona & Robinson, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, our group showed that the extracts of the plants did not produce any significant antigenotoxic effects in somatic cells of Drosophila melanogaster after acute treatments (Téllez et al, 2007;Toledo et al, 2008). The main constituents of the C. obtusifolia extracts were flavonoids and chlorogenic acid (Andrade-Cetto & Wiedenfeld, 2001).…”

Section: Research Articlementioning

confidence: 98%

Antimutagenic activity of two medicinal phytoextracts in somatic cells ofDrosophila melanogaster

Sortibrán

Téllez

Andrade‐Cetto

et al. 2011

Pharmaceutical Biology

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Genotoxicity testing of Cecropia obtusifolia extracts in two in vivo assays: The wing somatic mutation and recombination test of Drosophila and the human cytokinesis-block micronucleus test

Cited by 17 publications

References 18 publications

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement

Antimutagenic activity of two medicinal phytoextracts in somatic cells ofDrosophila melanogaster

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