Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test

Sarıkaya, Rabia; Çakir, Şükran

doi:10.1016/j.etap.2005.05.002

Cited by 38 publications

(30 citation statements)

References 24 publications

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“…The most common aberrations are chromatid breaks which indicates benzoic acid caused DNA double strand breaks and sister chromatid union which is the breakage followed by reunion of both sister chromatids at an identical site (Murli 2003). There are many studies that showed the genotoxicity of different food additives in different cell lines (Macioszek and Kononowicz 2004;Sarıkaya and Ç akır 2005;Yılmaz et al 2008;Mpountoukas et al 2008).…”

Section: Discussionmentioning

confidence: 99%

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

2009

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The chromosomal aberration (CA), sister chromatid exchange (SCE) and micronucleus test (MN) were employed to investigate the in vitro effect of antimicrobial food additive benzoic acid on human chromosomes. Lymphocytes were incubated with various concentrations (50, 100, 200 and 500 lg/ mL) of benzoic acid. The results of used assays showed that benzoic acid significantly increased the chromosomal aberration, sister chromatid exchange and micronucleus frequency (200 and 500 lg/mL) without changing the pH of the medium in a dosedependent manner. Also this additive significantly decreased the mitotic index (MI) at the highest concentration for 24 h and 100, 200 and 500 lg/mL for 48 h. This decrease was dose-dependent as well. However, it did not effect the replication (RI) and nuclear division (NDI) indices.

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Section: Discussionmentioning

confidence: 99%

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

2009

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“…In another study examining the genotoxic effects of the same substances with SMART, it was determined that all application groups displayed genotoxic effects at 50, 75, and 100 mM concentrations, whereas the groups obtained from a mixture of these substances displayed genotoxic effects at a 25 mM concentration (Sarıkaya and Çakır, 2005).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and safety assessment of 2 parabens in somatic cells of in vivo Drosophila melanogaster

Ayar¹,

Uysal

2013

Turk J Biol

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IntroductionWith the advent of technology, issues such as the development of different production methods in the food sector, the increase in the variety of food products, the desire to consume seasonal foods in every season, and the increasing of the shelf life of food products have made it obligatory to use food additives (FAs) (Ertuğrul, 1998).Protective FAs are defined as chemical substances that protect food products from deterioration caused by various microorganisms, thus increasing their shelf lives (Parlak, 2007). To this end, many FAs are used frequently in the food industry. One of these food additive groups is the parabens. The term "paraben" is an abbreviation for para-hydroxybenzoic acid. Parabens are a family of alkyl esters of para-hydroxybenzoic acid that differ at the para position of the benzene ring by various chemical substitutions (Sasseville, 2004). The chemical substitutions provide each paraben ester with a different solubility and spectrum of antimicrobial activity. As the alkyl chain length increases, water solubility decreases and oil solubility increases (Cashman and Warshaw, 2005) Parabens are a group of chemicals that are widely used as preserving additive substances in the food, cosmetic, and drug industries (Calafat et al., 2010). The most widely used parabens are methylparaben, ethylparaben, propylparaben, and butylparaben. Parabens are frequently used in bakery products (cakes, bread crust, fillers, etc.), drinks, fish products, aroma extracts, fruit products, gelatin, jam, gel, malt extracts, olives, pickles, salad sauces, syrups, and wine (Soni et al., 2002; CIR, 2008). Discussion regarding the safety of parabens has been going on for years within the scientific community. Many studies have put forth results stating that parabens are not toxic and that they can be used safely (Soni et al., 2005). However, with the detection of paraben traces in some breast tumors (Darbre et al., 2004) and various news items in the media regarding evidence that parabens are hazardous to human health, issues regarding the reliability of these substances have resurfaced.Today, it is now known whether many FAs including parabens have toxic effects or not and yet these substances continue to be used recklessly. One of the most widely used genotoxicity tests carried out on insects is the wing somatic mutation and recombination test (SMART) carried out with Drosophila (Demir et al., 2013). SMART enables the determination of the genetic results of various chromosome aberrations such as point mutation, deletion, translocation, somatic recombination, and chromosome loss or nondisjunction (Graf et al., 1984).

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“…Tennant (2009) observed the mutagenicity of tartrazine and has high ability to induce cancer in cells or tissues of multicellular animals in genetic change, and carcinogenesis. For genotoxicity in somatic mutation and recombination in D. melanogaster, five combinants at 25 mM concentration were evaluated (Sarikaya and Cakn, 2005).…”

Section: Experiments On the Genetic Toxicity Of Tartrazine Yellow And mentioning

confidence: 99%

EXPERIMENT ON THE GENETIC TOXICITY OF TARTRAZINE YELLOW AND BEHAVIORAL EFFECTS ON Drosophila melanogaster

El-Keredy

2018

Egyptian Journal of Genetics and Cytology

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Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test

Cited by 38 publications

References 24 publications

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

Genotoxic and safety assessment of 2 parabens in somatic cells of in vivo Drosophila melanogaster

EXPERIMENT ON THE GENETIC TOXICITY OF TARTRAZINE YELLOW AND BEHAVIORAL EFFECTS ON Drosophila melanogaster

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