Genotype comparison of <i>Candida albicans</i> isolates from different clinical samples

Bostănaru, Andra-Cristina; Roșca, Irina; Minea, Bogdan; Nastasa, Valentin; Foia, Liliana; Marincu, Iosif; Mareș, Mihai; Mederle, Ovidiu Alexandru

doi:10.2478/rrlm-2019-0027

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Normal vaginal flora may vary depending on the pH of the environment, age, hormonal status, sexual activity, method of contraception, drugs used, antibiotics, and surgical interventions (1). Candida species are distributed widely both in living organisms and in a wide variety of environments such as hospitals (2,3). These species are members of the human microbiome.…”

Section: Introductionmentioning

confidence: 99%

Vaginal candidiasis in Konya area: Etiology, risk factors, virulence patterns, and antifungal susceptibility

Tokak

Kılıç

Horasanlı

et al. 2021

Revista Romana De Medicina De Laborator

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Vulvovaginal candidiasis (VVC), a common genital tract infection, is known to affect millions of women worldwide. In this study, it was aimed to determine the prevalence, virulence, possible risk factors and antifungal susceptibility model of Candida species. Vaginal swab samples were taken from patients aged 18 years and older who presented to the gynecology outpatient clinic with signs and symptoms suggestive of vulvovaginitis. Demographic data were recorded using a questionnaire. Standard microbiological methods were used for the identification of the isolates. Broth microdilution method was used to determine the antifungal susceptibility of Candida isolates. Virulence factors of Candida strains were determined by performing proteinase, phospholipase, hemolytic and biofilm activity tests. Sequencing of the isolates identified as Candida were performed using ITS 1-4 primers. Vaginal discharge (OR: 3.365; 95% CI: 1.595-7.101), burning complaint (OR: 9.098; 95% CI: 2.284-36.232) and history of allergy (OR: 3.396; 95% CI: 0.968) were risk factors. The results showed that the most common isolated strain was Candida albicans (57%). It was found that the prevalence of C. glabrata remained at 26%, 44 of the C. albicans isolates presented proteinase, 35 had phospholipase, 47 had biofilm, and 47 had hemolytic activity. In this study, susceptible dose-dependent and resistant rates of all Candida strains were found for fluconazole as 9% and 16%, respectively. Host and organism-related factors should be considered in the clinical treatment of VVC, and continuous monitoring of changes in the prevalence of Candida species and susceptibility rates is required for effective antifungal therapy.

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Section: Introductionmentioning

confidence: 99%

Vaginal candidiasis in Konya area: Etiology, risk factors, virulence patterns, and antifungal susceptibility

Tokak

Kılıç

Horasanlı

et al. 2021

Revista Romana De Medicina De Laborator

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“…The goal of this work is to evaluate the status quo, as evidenced by expert opinions in the field, to narrow the topic, and to isolate and underline the impact of these tools on the resulting architectures and following bio-related potential. Although our research spans diverse topics, from bioagents’ carriers, biosynthesis [ 9 , 10 , 11 ], genotypic mapping [ 12 , 13 , 14 , 15 , 16 ], and cell growth [ 17 , 18 ], to artificial water channels [ 19 , 20 , 21 , 22 ], and the exploration of investigative methods [ 23 , 24 ], the review considers the hot topics of drug and gene delivery, antioxidants development, and bioimaging. We addressed these topics by following five major themes: (i) temperature- and pH-responsive systems; (ii) glycodynameric frameworks; (iii) polymeric non-viral vectors; (iv) metallic nanoconjugates; and, (v) smart organic tools.…”

Section: Introductionmentioning

confidence: 99%

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

2020

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Stimuli-responsive, “smart” polymeric materials used in the biomedical field function in a bio-mimicking manner by providing a non-linear response to triggers coming from a physiological microenvironment or other external source. They are built based on various chemical, physical, and biological tools that enable pH and/or temperature-stimulated changes in structural or physicochemical attributes, like shape, volume, solubility, supramolecular arrangement, and others. This review touches on some particular developments on the topic of stimuli-sensitive molecular tools for biomedical applications. Design and mechanistic details are provided concerning the smart synthetic instruments that are employed to prepare supra- and macro-molecular architectures with specific responses to external stimuli. Five major themes are approached: (i) temperature- and pH-responsive systems for controlled drug delivery; (ii) glycodynameric hydrogels for drug delivery; (iii) polymeric non-viral vectors for gene delivery; (iv) metallic nanoconjugates for biomedical applications; and, (v) smart organic tools for biomedical imaging.

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