Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.)

Myers, James R.; Grosser, Jude W.; Taylor, N. L.; Collins, G. B.

doi:10.1007/bf00035811

Cited by 30 publications

(15 citation statements)

References 32 publications

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“…Preliminary studies indicate that alfalfa in vitro response is strongly genotype dependent (Bingham et al, 1975;Atanassov & Brown, 1984). This feature was also reported by Myers et al (1989) working on Trifolium pratense L. Also, as a rule, growth regulators have a critical importance in inducing somatic embryogenesis in legumes. Atanassov & Brown (1984) reported the induction of callus from cotyledon, hypocotyl and leaf-derived alfalfa explants on B 5 h medium, which consists of B 5 medium (Gamborg et al, 1968) Bacto agar.…”

Section: Introductionmentioning

confidence: 70%

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Monteiro

Appezzato‐da‐Glória

Valarini

et al. 2003

Sci. agric. (Piracicaba, Braz.)

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Alfalfa is one of the most frequently studied species from the production of tissue culturederived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leafderived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

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Section: Introductionmentioning

confidence: 70%

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Monteiro

Appezzato‐da‐Glória

Valarini

et al. 2003

Sci. agric. (Piracicaba, Braz.)

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“…(Jacobsen & Kysely, 1985), Glycinae max (L.) Merr. (Komatsuda & Ohyama, 1988), Trifolium pratense L. (Myers et al ., 1989) but lower than in Medicago sativa L . (Suginobu et al ., 1991) .…”

Section: Formation Of Calli and Analysis Of Their Propertiesmentioning

confidence: 99%

Investigation of the fenugreek (Trigonella foenum-graecum L.) genotypes for fresh weight, seed productivity, symbiotic activity, callus formation and aceumulation of steroids

Проворов

Soskov²,

Лутова

et al. 1996

Euphytica

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As a result of the analysis of 31 fenugreek (Trigonella foenum-graecum L.) genotypes we demonstrated that subsp . indica surpass significantly subsp . foenum-graecum in fresh weight, seed yield, nodulation ability, resistance to root rot and the duration of the interstage and growing periods . There was no difference between the subspecies for leafiness and biochemical composition of seeds (concentrations of diosgenin and steroid glucosides, nitrogen, fat, inhibitors of tripsin and hemotripsin) . The most promising genotypes (as compared with the standard cv. Nakhichevanskaya Shambala) yield 1650-1938 g/m 2 of fresh weight, 98 .2-116 .1 g/m2 of seeds and accumulate 1 .45-1 .64% of diosgenin in seeds . High positive correlations (r = + 0 .53 . . . + 0.84 ; P,, < 0.01) were found between fresh weight, seed yield, plant leafiness and height. Inoculation of the cv . Nakhichevanskaya Shambala with Rhizobium meliloti strains 851 and 852 increased its fresh weight by 94 .5% and 93 .6% and seed yield by 83 .1 % and 60.5%, respectively. Significant correlations of the nodule number with the fresh weight and seed yield (r = + 0 .70 and + 0 .77 ; P,, < 0 .01) allow us to assume that selection for increased symbiotic activity may be useful for improving the fenugreek productivity . The intersubspecies hybrids (four genotypes) surpass the subsp . indica (six genotypes) for the sizes of calli formed in presence of 2 .4-D and kinetin. The sizes of calli formed by ten analysed genotypes in presence of BAP and kinetin (or 2 .4-D) were negatively correlated with the nodule number (or fresh weight) .

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“…However, before foreign genes can be introduced it is essential that an efficient regeneration protocol be established. There are several reports that describe regeneration protocols for a number of Trifolium species including red clover (T. pratense L.) (Phillips & Collins 1980;Myers et al 1989), crimson clover (T. incarnatum L.) (Beach & Smith 1979), zigzag clover (T. medium L.) (Choo 1988), foxtail clover (T. rubens L.) (Parrott & Collins 1982) and white clover (T. repens L.) (Bond & Webb 1989;White 1984;Gresshoff 1980). For T. subterraneum, regeneration via somatic embryogenesis (Maheswaran & Williams 1986) and shoot organogenesis (Webb et al 1987) has been described.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration of commercial cultivars of subterranean clover

Heath

Chin

Spencer

et al. 1993

Plant Cell Tiss Organ Cult

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Regeneration of subterranean clover (Trifolium subterraneum L.) was achieved by both shoot organogenesis and somatic embryogenesis. Shoots derived via organogenesis were initiated from the hypocotyls of mature imbibed seed. The hypocotyl, including the emerging radicle, was sliced longitudinally into two halves and cultured on shoot induction medium. After 30 days, adventitious shoots were formed from the hypocotyl region while the radicle showed no development. Shoots were then subcultured onto shoot multiplication medium and finally onto a root initiation medium. Histological studies revealed that shoots arose de novo and did not originate from pre-existing meristems. In the second regeneration protocol, shoot apical meristems from young seedlings were induced to form callus. Following four to six weeks culture in the dark, somatic embryos appeared spontaneously on the calli. A majority of embryos had a well-defined root pole, two cotyledonary lobes, and were capable of germination, albeit at a low frequency. Regenerated plants obtained from both protocols appeared phenotypically normal.

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Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.)

Cited by 30 publications

References 32 publications

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

Investigation of the fenugreek (Trigonella foenum-graecum L.) genotypes for fresh weight, seed productivity, symbiotic activity, callus formation and aceumulation of steroids

In vitro regeneration of commercial cultivars of subterranean clover

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