Investigation of the fenugreek (Trigonella foenum-graecum L.) genotypes for fresh weight, seed productivity, symbiotic activity, callus formation and aceumulation of steroids

Abstract

As a result of the analysis of 31 fenugreek (Trigonella foenum-graecum L.) genotypes we demonstrated that subsp . indica surpass significantly subsp . foenum-graecum in fresh weight, seed yield, nodulation ability, resistance to root rot and the duration of the interstage and growing periods . There was no difference between the subspecies for leafiness and biochemical composition of seeds (concentrations of diosgenin and steroid glucosides, nitrogen, fat, inhibitors of tripsin and hemotripsin) . The most promising genotypes (as compared with the standard cv. Nakhichevanskaya Shambala) yield 1650-1938 g/m 2 of fresh weight, 98 .2-116 .1 g/m2 of seeds and accumulate 1 .45-1 .64% of diosgenin in seeds . High positive correlations (r = + 0 .53 . . . + 0.84 ; P,, < 0.01) were found between fresh weight, seed yield, plant leafiness and height. Inoculation of the cv . Nakhichevanskaya Shambala with Rhizobium meliloti strains 851 and 852 increased its fresh weight by 94 .5% and 93 .6% and seed yield by 83 .1 % and 60.5%, respectively. Significant correlations of the nodule number with the fresh weight and seed yield (r = + 0 .70 and + 0 .77 ; P,, < 0 .01) allow us to assume that selection for increased symbiotic activity may be useful for improving the fenugreek productivity . The intersubspecies hybrids (four genotypes) surpass the subsp . indica (six genotypes) for the sizes of calli formed in presence of 2 .4-D and kinetin. The sizes of calli formed by ten analysed genotypes in presence of BAP and kinetin (or 2 .4-D) were negatively correlated with the nodule number (or fresh weight) .

Phenotypic variation within a fenugreek (Trigonella foenum-graecum L.) germplasm collection. II. Cultivar selection based on traits associated with seed yield

“…For Experiment 1, estimates of predicted means and standard errors for variates with significant spatial effects were obtained using the linear variance neighbour analysis procedure VAR-MODEL in GENSTAT (Payne et al 1993). For variates showing little spatial variation, estimates of predicted means and standard errors were obtained using REML with accession as a fixed effect and no random effects.…”

“…All analyses were conducted using GENSTAT 5.3 and 5.4 (Payne et al 1993). For Experiment 1, estimates of predicted means and standard errors for variates with significant spatial effects were obtained using the linear variance neighbour analysis procedure VAR-MODEL in GENSTAT (Payne et al 1993).…”

“…Broad-sense heritability and its standard error were estimated using the VFUNCTION procedure in GENSTAT (Payne et al 1993) for the traits assessed. Broad-sense heritability (h 2 ) was calculated at the experiment level as the genotypic variance divided by the phenotypic variance.…”

Respuesta de diferentes variedades de cereales a la inoculación con Bradyrhizobium sp.

“…Pers) could respond to a previous selection of the first on the basis of heavy doses of fertilizer to obtain seeds, influencing the form of autotrophic nutrition of the plants (Table 3). This phenomenon has been previously reported by Próvorov et al (1996) in the selection of genotypes of Trigonella foenum-graecum L., inoculated with Rhizobium meliloti. We must never ignore the fact that statistically inoculated triticale, triticale-fertilized than in that variable and shared common superscripts IRM37-fertilized treatments, IRM37-inoculated and IRM32-fertilized, but also did it with triticaleabsolute control, which could indicate that the seed filling in this range, due to the influence of indigenous rhizosphere bacteria was carried out as efficiently as by the action of the strain introduced.…”

“…Este fenómeno ha sido anteriormente informado por Próvorov et al (1996) en la selección de genotipos de Trigonella foenum-graecum L., inoculada con Rhizobium meliloti. No se debe obviar, el hecho de que triticaleinoculado resultó estadísticamente superior a triticalefertilizado en dicha variable, y que compartió superíndices comunes con los tratamientos IRM37-fertilizado, IRM37-inoculado e IRM32-fertilizado, aunque también lo hizo con triticale-control absoluto, lo cual pudiera indicar que el llenado de las semillas en esa variedad, debido a la influencia de las bacterias rizosféricas autóctonas, se realizó de forma tan eficiente como por la acción de la cepa introducida.…”