Based on the statistical assessment of long-term field studies, in competitive variety tests, barley cultivars were identified adaptive to the conditions of the region, promising for transfer to the State variety test. As a result of the studies, a promising breeding material was created. The efficiency of using various methods of creating a new initial material, in particular, the cell culture method, is shown. A statistically significant correlation was established between the value of the hydrothermal coefficient (GTK) in the period “leaf tube formation - heading” and the yield capacity of a cultivar (r = 0.520). High variability (V = 24.4%) in the duration of the inter-stage period “leaf tube formation - heading” was revealed in the cultivars of competitive variety testing. Cultivars 94-13 and 38-15 are distinguished by a high yield potential (up to 6.4… 6.9 t/ha) and the duration of inter-stage periods, at which the main stages of organogenesis occur at the most favorable combination of heat and moisture for the potential development of plant features and properties. Cultivars 207-15, 38-15, and regenerant 550-08, characterized by high annual average yield capacity (5.9…5.7 tons/ha) and adaptability, are promising for transfer to the Russian Federation State variety test.