Genotypes and Antibiotic Resistances of Campylobacter jejuni Isolates from Cattle and Pigeons in Dairy Farms

Bianchini, Valentina; Luini, M.; Borella, Laura; Parisi, Antonio; Jonas, Romie; Kittl, Sonja; Kuhnert, Peter

doi:10.3390/ijerph110707154

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…This CC is frequently associated with cases of human disease and is one of the most frequently reported CCs in poultry (Dingle et al., 2001 ; Kärenlampi et al., 2007 ; Kittl et al, 2013b ; Manfreda et al., 2016 ; EFSA and ECDC, 2021 ); however, CC‐21 has also been found in cattle (Pezzotti et al., 2003 ; EFSA and ECDC, 2021 ), sheep (Lucarelli et al., 2016 ), turkey (Lo Schiavo et al., 1991 ) and the environment (Dingle et al., 2001 ). Several studies were performed in Italy and show how common this CC is not only in chickens but also in strains isolated from turkeys, cattle and bulk tank milk (Lo Schiavo et al., 1991 ; Bianchini et al., 2014a , b ). The most common CC among chicken C. jejuni isolates was the CC‐21 according to that documented worldwide (Pezzotti et al., 2003b ; EFSA and ECDA, 2014 ; Lucarelli et al., 2016 ; Cito et al., 2018 ; Sarhangi et al., 2021 ).…”

Section: Results and Conclusionmentioning

confidence: 99%

Monitoring AMR in Campylobacter jejuni from Italy in the last 10 years (2011–2021): Microbiological and WGS data risk assessment

Conesa

Garofolo

Pasquale

et al. 2022

EFS2

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Campylobacter jejuni is considered as the main pathogen in human food‐borne outbreaks worldwide. Over the past years, several studies have reported antimicrobial resistance (AMR) in C. jejuni strains. In Europe, the official monitoring of AMR comprises the testing of Campylobacter spp. from food‐producing animals because this microorganism is responsible for human infections and usually predominant in poultry. Food‐producing animals are considered to be a major source of campylobacteriosis through contamination of food products. Concerns are growing due to the current classification of C. jejuni by the WHO as a ‘high priority pathogen’ due to the emergence of resistance to multiple drugs such as those belonging to the fluoroquinolones, macrolides and other classes, which limits the treatment alternatives. Knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritise food safety interventions and to establish proper control strategies. Assessing the genetic diversity among Campylobacter species is essential to the understanding of their epidemiology and population structure. Using a population genetic approach and grouping the isolates into sequence types within different clonal complexes, it is possible to investigate the source of the human cases. The work programme was aimed for the fellow to assess the AMR of C. jejuni isolated from humans, poultry and birds from wild and urban Italian habitats. Given the public health concern represented by resistant pathogens in food‐producing animals and the paucity of data about this topic in Italy, the aim was to identify correlations between phenotypic and genotypic AMR and comparing the origin of the isolates. The work programme allowed the fellow to acquire knowledge, skills and competencies on the web‐based tools used by IZSAM to process the NGS data and perform bioinformatics analyses for the identification of epidemiological clusters, the study of AMR patterns in C . jejuni isolates, and the assessment of the human exposure to such AMR pathogens. Furthermore, the fellow became able to transfer the acquired knowledge through innovative web‐based didactical tools applied to WGS and clustering of specific food‐borne pathogens, with particular reference to C . jejuni . To achieve this objective, 2,734 C. jejuni strains isolated from domestic and wild animals and humans, during the period 2011–2021 were analysed. The resistance phenotypes of the isolates were determined using the microdilution method with EUCAST breakpoints, for the following antibiotics: nalidixic acid, ciprofloxacin, chloramphenicol, erythromycin, gentamicin, streptomycin, tetracycline. The data were complemented by WGS data for each strain, uploaded in the Italian information system ...

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Section: Results and Conclusionmentioning

confidence: 99%

Monitoring AMR in Campylobacter jejuni from Italy in the last 10 years (2011–2021): Microbiological and WGS data risk assessment

Conesa

Garofolo

Pasquale

et al. 2022

EFS2

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“…In a recent survey conducted in Northern Italy, C. jejuni was detected in 12% of the bulk tank milk samples that were examined ( Bianchini et al, 2014 ), and some disease outbreaks have been reported in the same Italian regions (two in Emilia Romagna, one in Veneto, and one in Marche) following the consumption of raw milk from self-service automatic vending machines ( Amato et al, 2007 ; Arrigoni et al, 2009 ; Petruzzelli et al, 2011 ). Despite no official data existing for the incidence of campylobacteriosis associated with raw milk consumption, quantitative risk assessment modeling has estimated that the worst possible scenario for human infections with C. jejuni linked to the consumption of raw milk in Italy would exceed 300,000 cases per year ( Giacometti et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source

et al. 2016

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Food-borne campylobacteriosis is caused mainly by the handling or consumption of undercooked chicken meat or by the ingestion of contaminated raw milk. Knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritize food safety interventions and to establish proper control strategies. Assessing the genetic diversity among Campylobacter species is essential to our understanding of their epidemiology and population structure. We molecularly characterized 56 Campylobacter jejuni isolates (31 from patients hospitalized with gastroenteritis, 17 from raw milk samples, and 8 from chicken samples) using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) in order to trace the source of the disease. We also used a population genetic approach to investigate the source of the human cases from six different reservoirs of infection. MLST identified 25 different sequence types and 11 clonal complexes (CCs) (21, 658, 206, 353, 443, 48, 61, 257, 1332, 354, 574) and these included several alleles not cited previously in the PubMLST international database. The most prevalent CCs were 21, 206, and 354. PFGE showed 34 pulsotypes divided between 28 different clusters. At the fine scale, by means of PFGE and MLST, only two human cases were linked to raw milk, while one case was linked to chicken meat. The investigation revealed the presence of several genotypes among the human isolates, which probably suggests multiple foci for the infections. Finally, the source attribution model we used revealed that most cases were attributed to chicken (69.75%) as the main reservoir in Italy, followed to a lesser extent by the following sources: cattle (8.25%); environment (6.28%); wild bird (7.37%); small ruminant (5.35%), and pork (2.98%). This study confirms the importance of correlating epidemiological investigations with molecular epidemiological data to better understand the dynamics of infection.

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“…It was previously reported that prevalent STs of C. jejuni and C. coli isolated from cattle in Japan were ST806 and ST21, and ST1068 (Asakura et al 2012;Asakura et al 2019;Sasaki et al 2020). ST21 of C. jejuni and ST1068 of C. coli are also prevalent in cattle in Italy, USA., and the UK, demonstrated that the host associations of Campylobacter genotypes are more robust than their geographic variations (Kwan et al 2008;Sanad et al 2011;Roux et al 2013;Bianchini et al 2014;Jonas et al 2015;Cha et al 2017;Sheppard et al 2010). The top three STs of C. jejuni and the most frequent ST (ST1068) of C. coli would be adaptable to beef cattle and widely spread to beef farms in Japan.…”

Section: Open Accessmentioning

confidence: 99%

Prevalence and fluoroquinolone resistance of Campylobacter spp. isolated from beef cattle in Japan

2022

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Beef is a source of human Campylobacter infections. Antimicrobial treatment is needed when patients are immunocompromised or have other comorbidities. Therefore, we investigated the prevalence and antimicrobial resistance of Campylobacter spp. in beef cattle in Japan. Rectal swab samples were collected from 164 beef cattle at an abattoir between March 2021 and August 2021, and Campylobacter spp. were isolated from 94 (57.3%) cattle. C. jejuni and C. coli were isolated from 68 and 26 cattle, respectively. For Campylobacter jejuni, the resistant rates against ampicillin, tetracycline and ciprofloxacin were 20.6, 75.0 and 64.7%, respectively. For C. coli, the resistant rates against ampicillin, tetracycline and ciprofloxacin were 53.8, 76.9 and 88.5%, respectively. No Campylobacter isolates were resistant to erythromycin. By multilocus sequence typing, C. jejuni and C. coli isolates were classified into 22 and 2 sequence types (STs). The top three STs of C. jejuni were ST806 (12 isolates), ST21 (nine isolates), and ST459 (eight isolates). The most frequent ST of C. coli was ST1068 (23 isolates). The results suggest that Campylobacter spp. are prevalent in the gastrointestinal tract of beef cattle slaughtered at abattoirs. Furthermore, the administration of erythromycin is effective against human campylobacteriosis caused by beef consumption. Monitoring the prevalence and antimicrobial resistance of Campylobacter spp. in beef cattle could be useful for managing the risk of human campylobacteriosis.

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Genotypes and Antibiotic Resistances of Campylobacter jejuni Isolates from Cattle and Pigeons in Dairy Farms

Cited by 9 publications

References 19 publications

Monitoring AMR in Campylobacter jejuni from Italy in the last 10 years (2011–2021): Microbiological and WGS data risk assessment

Monitoring AMR in Campylobacter jejuni from Italy in the last 10 years (2011–2021): Microbiological and WGS data risk assessment

Tracing Back Clinical Campylobacter jejuni in the Northwest of Italy and Assessing Their Potential Source

Prevalence and fluoroquinolone resistance of Campylobacter spp. isolated from beef cattle in Japan

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