Genotypes and clinical aspects associated with bone mineral density in Argentine postmenopausal women

Abstract

The aim of this study was to determine genotypes and clinical aspects associated with bone mineral density (BMD) in postmenopausal women from Córdoba, Argentina. Polymorphisms were assessed by RFLP-PCR technique using BsmI and FokI for vitamin D receptor gene (VDR) and XbaI and PvuII for estrogen receptor-alpha gene (ERalpha) as restrictases. Sixty-eight healthy, 54 osteopenic, and 64 osteoporotic postmenopausal women were recruited. Femoral neck and lumbar spine BMD were inversely correlated with age in the entire analyzed population. Height was lower in osteopenic and osteoporotic women as compared to healthy women (P < 0.05). Weight and body mass index (BMI) were the lowest in osteoporotic women (P < 0.01 versus healthy group). Serum procollagen type I Nterminal propeptide (PINP) was higher in osteoporotic women as compared to the other groups. Distribution of VDR and ERalpha genotypes was similar in the three groups. Genotype bb (VDR) was associated with low values of lumbar BMD in the healthy group (P < 0.05 versus genotype Bb), and with low values of femoral BMD (P < 0.05 versus genotype BB) in osteoporotic women. BB*Pp interaction was associated with the highest femoral neck BMD (P < 0.05), whereas the bb*xx interaction was associated with the lowest femoral neck BMD in the total population analyzed (P < 0.05). In conclusion, parameters such as age, height, weight, BMI, serum PINP, VDR genotypes, and interactions between VDR and ERalpha genotypes could be useful to predict a decrease in BMD in Argentine postmenopausal women.

Vitamin D Receptor and Calcium-Sensing Receptor Gene Polymorphisms in Hypercalciuric Stone-Forming Patients

“…On the other hand, although several studies in the past have suggested that multiple VDR polymorphisms (including BsmI ) influence the regulation of BMD [12,13,14,15], a more recent multicenter prospective trial [17] involving around 26,000 participants, as well as other studies [18,19], have not confirmed such an association. Two additional studies reported an association between bb genotype with low rather than higher BMD [20,62]. …”

The role of vitamin D receptor gene polymorphisms in the bone mineral density of Greek postmenopausal women with low calcium intake

“…Although this initial finding has been partially withdrawn, in the following years, a large number of studies investigated the potential associations of VDR polymorphisms with BMD in different ethnic populations [4][5][6][7][8][9][10], as well as with other parameters such as bone loss [11,12], fractures [11,13,14] and bone turnover [14][15][16]. However, these studies have provided controversial and inconclusive results due to differences in sample size, ethnic background and age group [17,18].…”

Molecular mechanisms triggered by low-calcium diets

“…21 reabsorption (14) . This is an adaptation process performed as a compensation mechanism in order to cover the cation needs of the organism (15) . Serum Ca 2þ levels may be normal or low, according to the extent and degree of Ca 2þ deficiency.…”